GC-MS ANALYSIS & MOLECULAR DOCKING STUDIES OF laserpitium LATIFOLIUM L EXTRACT FOR ANTI-DIABETIC ACTIVITY
DOI:
https://doi.org/10.22159/ijap.2025v17i1.52343Keywords:
Lasterpitium latifolium L, Alpha-amylase and alpha-glucosidase, 2,4-Thiazolidinedione, 5- [[4-(4-pyridinyl)-6- quinolinyl] methylene] -(5Z)-, Cycloartenol acetateAbstract
Objective: To assess the anti-diabetic activity of Lasterpitium latifolium L extract through computational docking studies.
Methods: Crude extract of Lasterpitium latifolium L whole plant was obtained using a rotary evaporator. The extract was analyzed for bioactive compounds using Gas chromatography/Mass spectrometry (GC-MS). The extract was examined for their anti-diabetic effects through computational docking studies and in‑vitro. For docking studies, proteins of diabetes mellitus, α-amylase and α-glucosidase, were selected from the literature.
Results: Binding affinity assessed by score function identified 2,4-Thiazolidinedione, 5- [[4-(4-pyridinyl)-6- quinolinyl] methylene] -(5Z)- (highest docking score of -7.8 kcal/mol) and cycloartenol acetate (docking score of -7.5kcal/mol) are the most promising compounds that showed strong affinity to target proteins of diabetes mellitus. These compounds have the highest docking scores, suggesting that they are potential candidates for anti-diabetic drug development. Extract of L. latifolium exhibited 68.1% potent activity at 250 μg/mL.
Conclusion: In the anti-diabetic potential of crude extracts, multiple pancreatic and extra pancreatic mechanisms may occur synergistically to achieve a strong anti-diabetic effect.
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