Innovare Journal of Sciences

POTENTIAL OF MYCORRHIZAL INOCULATION AND CATTLE RUMEN DIGESTA IN THE BIOREMEDIATION OF SPENT ENGINE OIL CONTAMINATED SOIL

2023-12-16T18:54:02+0530

Soil pollution by crude oil contamination has become a major constraint on agricultural productivity. Physicochemical techniques are often expensive. However, bioremediation of petroleum hydrocarbon polluted soil is cost-effective. Therefore, the study was carried out to analyze the influence of mycorrhiza and cattle rumen digesta on bioremediation of Spent Engine Oil (SEO) contaminated soil in Dutse, Jigawa state. Soil samples were randomly collected from the University Research and Teaching Farm. About 2.5 kg of sterilized topsoil (0–15 cm) was filled into pots and arranged in a 2×2×3 factorial experiment in completely randomized design with three replications. Mycorrhiza and cattle rumen digesta were at two levels, while SEO was at three levels. Data were collected on the total petroleum hydrocarbon (TPH) content, bacterial and fungal colony count. Data were analyzed using ANOVA at α 0.05. Results obtained from the study show that mycorrhiza and cattle rumen digesta increased the colonies of fungi and bacteria resulting in significantly enhanced TPH degradation in the contaminated soil. However, cattle rumen digesta significantly (p<0.05) enhanced TPH degradation, bacterial and fungal population the most compared to mycorrhiza alone. Combined cattle rumen digesta and mycorrhiza application resulted in significantly (p<0.05) lower residual TPH content in the contaminated soil compared to using cattle rumen digesta or mycorrhiza alone. Thus, cattle rumen digesta and mycorrhiza should be used in bioremediation of petroleum hydrocarbon impacted soils.

DETECTION OF BACTERIAL CONTAMINANTS IN READY-TO-DRINK BEVERAGE (ZOBO) SOLD ON FEDERAL UNIVERSITY DUTSE CAMPUS, NORTHWEST NIGERIA

2023-12-16T22:10:14+0530

Introduction: Consumption of ready-to-drink beverages is a norm in the world over. However, the presence of total coliform in any beverage renders it unfit for human consumption.

Objectives: Due to non-existence of scientific data on the safety status of the ready-to-drink Zobo which is a popular local beverage, we did conduct this study to assess the bacteriological quality of the lots sold to consumers on the campus of Federal University Dutse (FUD), Nigeria.

Methods: Purposive sampling was employed to sample nine (9) locally packaged Zobo in three (3) different outlets; FUD Bakery (BAK) where three (3) were assayed (BAKA, BAKB, and BAKC), FUD Backside (BS) where three (3) samples were assayed (BSA, BSB, and BSC), and Morocco Girls’ hostel (MGH) three (3) samples were assayed (MGHA, MGHB, and MGHC). Standard methods were used to assay the sampled Zobo.

Results: Results obtained indicate that samples from MGH (8.0×10⁴ CFU/mL) had the lowest total viable count (TVC), while samples from BAK (2.8×10⁷ CFU/mL) recorded the highest TVC. However, samples assayed from MGH recorded no total coliform count. The number of bacterial colonies recorded in BAKA and BAKB was not significantly different from each other (p>0.05). However, the number of bacterial colonies recorded from MGHA was significantly different from those obtained in MGHB and MGHC (p<0.05).

Conclusion: Due to the results obtained, we can conclude that Zobo samples assayed in the sampling points were not fit for human consumption during the conduct of our study.