EVALUATION OF THE FOLK CLAIM AND IDENTIFICATION OF THE PHARMACOLOGICAL ACTIVE PRINCIPLES IN BAUHINIA PHOENICEA LEAVES

Authors

  • Alby Alphons Baby R&D Centre Bharathiar University Coimbatore
  • Regi Raphael K st. Mary's college Thrissur

Abstract

ABSTRACT
Objective: Bauhinia phoenicea, an endemic plant of western Ghats, using in traditional medicine against diabetes, skin allergies, fungal infections, and
worm disturbances. To the best of our knowledge, any scientific studies on the medicinal properties of the leaves of this plant are not yet reported.
Therefore, as the first step toward unraveling its medicinal property, bioactivity profiling, and gas chromatography-mass spectrometry (GC-MS)
analysis were performed.
Methods: The pharmacological activity profiling includes antibacterial, antifungal, anthelmintic, and antioxidant property screening using crude
ethanolic extract, which preliminary analyses its folk claim. GC-MS analysis performed to identify the pharmacologically active principles. All analyses
were performed according to standard protocols.
Results: Ethanol extract of the leaves of B. phoenicea was assayed for the antimicrobial activity against 10 human pathogenic strains using well
diffusion assay. The extract showed a significant activity against all pathogens. A maximum zone of inhibition observed in Salmonella typhi in their
higher concentration (500 µg/ml). The anthelmintic activity of crude drug evaluated on Indian adult earthworms Pheretima posthuma, exhibited dosedependent
spontaneous mortality,
and evoked
responses
to
pin prick
and effects
compared
with that of albendazole. The drug showed
potent
antioxidant

property
in 2,2-diphenyl-1-picrylhydrazyl
free
radical
scavenging
and superoxide
anion scavenging
assays
with half maximal inhibitory
concentration

values
92±0.92 and 62±1.34, respectively.
The GC-MS
analysis
which
showed
the presence
of 19 compounds including hexadecanoic
acid and oleic acid.
Conclusion: According to our results, it is concluded that leaf of B. phoenicea has significant antimicrobial, anthelmintic, and antioxidant properties
supporting the folk medicinal use of this species. The further procedures of isolation and characterization of active principles are in progress.
Keywords: Bauhinia phoenicea, Antimicrobial, Anthelmintic, Antioxidant property and gas chromatography-mass spectrometry.

Downloads

Download data is not yet available.

Author Biography

Alby Alphons Baby, R&D Centre Bharathiar University Coimbatore

Research Scholar

St. Mary's College

Thrissur

References

REFERENCES

Nostro A, Germanò MP, D’angelo V, Marino A, Cannatelli MA.

Extraction methods and bioautography for evaluation of medicinal

plant antimicrobial activity. Lett Appl Microbiol 2000;30(5):379-84.

Sibanda T, Okoh AL. The challenges of overcoming antibiotic resistance;

Plant extracts as potential source of antimicrobial and resistance

modifying agents. Afr J Biotechnol 2007;6 Suppl 25:2886-962.

Smith EC, Williamson EM, Wareham N, Kaatz GW, Gibbons S.

Antibacterials and modulators of bacterial resistance from the immature

cones of Chamaecyparis lawsoniana. Phytochemistry 2007;68

Suppl 2:210-7.

Bandow JE, Brötz H, Leichert LI, Labischinski H, Hecker M.

H

O 296 2.36

11.52 Ã -D-glucopyranose, 4-O-Ã -D-galactopynnosyl- C

H

O

52.10

12.18 n-hexadecanoic acid C

H

O

8.52

13.31 9,12-octadecadicnoic acid, methyl ester, (E, E)- C

H

O

0.86

13.36 Oleic acid C

H

O

1.03

13.51 Phytol C

H

O 296 5.06

14.18 9,12-octadecadicnoic acid (Z, Z)- C

H

O

12.41

19.02 1,2-benzenedicarboxyllc acid, mono (2-ethylhexyl) ester C

H

O

0.73

21.50 13,16-octadecadiynotc acid, methyl ester C

H

O

0.34

22.48 Squalene C

H

410 3.32

24.51 2H-1-benzopyran-6-ol,

,4-dihydro-2,8-dimethyl-2-(4,8,12-trimethyltridecyl)-, [2R-[2R*(4R*,

R*)]], (&-Tocopherol)

26.03 8,11,14-eicosatricnok acid, (Z, Z, Z)- C

C

H

H

O

O

0.58

26.70 Vitamin E C

H

O

1.53

26.84 8-dodecen-l-ol, acetate, (Z)- C

H

O

0.56

29.19 5,8,11,14-eicosatetraenoic acid, methyl ester, (all-Z)- C

H

O

2.92

28.32 8,11,14-eicosatricnok acid, (Z, Z, Z)- C

H

O

0.64

29.19 2H-Pyran, 2-(7-heptadecynyloxy) tetrahydro- C

J

O

2.92

29.98 Cholestan-3-ol, 2-methylene-, (3Ã ,5Ã ) C

H

O 400 1.81

30.52 Cyclopropaneoctanok acid, 2-([2-[(2- ethylcyclopropyI) methyl]

cyclopropyl] methyl]-, methyl ester

C

B. phoenicea: Bauhinia phoenicea

H

O

1.26

1.07

Proteomic approach to understanding antibiotic action. Antimicrob

Agents Chemother 2003;47(3):948-55.

Colombo ML, Bosisio E. Pharmacological activities of Chelidonium

majus L. (Papaveraceae). Pharmacol Res 1996;33:127-34.

Baby AA, Raphael KR. Pharmacognostic features of an endemic

traditional medicine Bauhinia phoenicea Wight & Arn leaves. World J

Pharm Pharm Sci 2014;3 Suppl 11:479-85.

Baby AA, Raphael KR. First step towards unraveling the medicinal

properties of an endemic traditional medicine, Bauhinia phoenicea

Wight & Arn bark. Int J Pharm Pharm Sci 2015;7(9):403-05.

Harbone JB. Phytochemical Methods, A Guide to Modern Techniques

of Plant Analysis. India: Springer Pvt., Ltd.; 1998. p. 5-12.

Rojas JJ, Ochoa VJ, Ocampo SA, Muñoz JF. Screening for antimicrobial

activity of ten medicinal plants used in Colombian folkloric medicine:

A possible alternative in the treatment of non-nosocomial infections.

BMC Complement Altern Med 2006;6:2.

du Toit EA, Rautenbach M. A sensitive standardised micro-gel

well diffusion assay for the determination of antimicrobial activity.

J Microbiol Methods 2000;42(2):159-65.

Vital PG, Velasco RN, Demigillo JM, Rivera WL. Antimicrobial

activity, cytotoxicity, and phytochemical screening of Ficus septica

Burm and Sterculia foetida L. leaf extracts. J Med Plant Res 2010;4

Suppl 1:58-63.

Baby AA, Raphael KR. Potential antimicrobial, anthelmintic and

antioxidant properties of Areca catechu L root. Int J Pharm Pharm Sci

;6 Suppl 6:486-9.

Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant

activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol

;79(3):379-81.

Deb L, Dubey SK, Jain A, Jain AK, Pandian GS. Preventive effect of

Thuja occidentalis (linn) on gastric ulcer - A novel role of free radical

scavenger. J Nat Remedies 2009;9(2):152-8.

Published

01-07-2016

How to Cite

Baby, A. A., and R. R. K. “EVALUATION OF THE FOLK CLAIM AND IDENTIFICATION OF THE PHARMACOLOGICAL ACTIVE PRINCIPLES IN BAUHINIA PHOENICEA LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 104-7, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/%60.

Issue

Section

Original Article(s)