ANTIBACTERIAL INTERACTION OF COMBINATION OF ETHANOLIC EXTRACT OF ZINGIBER OFFICINALE VAR RUBRUM RHIZOME, BOESENBERGIA PANDURATA RHIZOME, AND STEVIA REBAUDIANA LEAVES WITH CERTAIN ANTIBIOTICS AGAINST INFECTIOUS MOUTH MICROBIAL

Authors

  • Elin Yulinah Sukandar School of pharmacy, Bandung Institute of technology
  • Neng Fisheri Kurniati School of pharmacy, Bandung Institute of technology
  • Pratiwi Wikaningtyas School of pharmacy, Bandung Institute of technology
  • Deani Agprikani School of pharmacy, Bandung Institute of technology

Abstract

Objectives: The present study was performed to show the in vitro activities of ethanolic extract of Zingiber officinale var rubrum rhizome, Boesenbergia
pandurata rhizome, and Stevia rebaudiana leaves in combination with amoxicillin, vancomycin, or ketoconazole against infectious mouth microbial.
Methods: The antibacterial activities were calculated based on minimum inhibitory concentration (MIC) using microdilution method and minimum
bactericidal concentration, and the antifungal activity were calculated using minimum fungicidal concentration using agar diffusion method. The
extract-antibiotic interaction was performed using checkerboard method.
Results: Among the three extracts studied, B. pandurata rhizome extract had the best antimicrobial activity against S. aureus and S. mutans with
MIC value at 128 µg/mL and 64 µg/mL, respectively while Z. officinale rhizome extracts had the best antifungal activity against C. albicans with MIC
value at 2048 µg/mL. The antimicrobial interaction between vancomycin with S. rebaudiana against S. aureus and S. mutans showed a synergistic
effect. Synergism also observed in the combination of amoxicillin with B. pandurata or S. rebaudiana against S. aureus and S. mutans, respectively.
The combination of ketoconazole with each of all plant extracts studied against C. albicans showed the synergistic effect. The additive interaction
was observed between amoxicillin with B. pandurata or S. rebaudiana against S. aureus and S. mutans, respectively. There was no antagonism being
observed in this study.
Conclusion: There is a synergistic interaction between plant extract and the antibiotic against S. aureus, S. mutans, and C. albicans, therefore, the
combinations may be useful for infectious mouth microbial treatment.
Keywords: Antimicrobial, Interaction, Zingiber officinale var rubrum, Stevia rebaudiana, Boesenbergia pandurata, Ketoconazole, Amoxicllin,
Vancomycin, Minimum inhibition concentration, Microdilution, Synergistic, Additive.

Downloads

Download data is not yet available.

References

Jebashree HS, Kingsley SJ, Sathish ES, Devapriya D. Antimicrobial Activity of Few Medicinal Plants against Clinically Isolated Human Cariogenic Pathogens—An In Vitro Study. ISRN Dentistry2011;1-15.

Grubb SEW, Murdoch C, Sudbery PE, Saville SP, Lopez-Ribot JL, Thornhill MH. Candida albicans-Endothelial Cell Interactions: a Key Step in the Pathogenesis of Systemic Candidiasis . Infection and Immunity 2008;76(10):4370-4377

Marrelli M, Tatullo M, Dipalma G, Inchingolo F. Oral Infection by Staphylococcus Aureus in Patients Affected by White Sponge Nevus: A Description of Two Cases Occurred in the Same Family. International Journal of Medical Sciences 2012;9(1):47-50.

Greenberg, M.S and Glick M, 2008,Burket’s Oral Medicine. 11th ed. Ontario, BC Decker Inc, 79-82.

Sukandar EY, Sunderam N, and Fidrianny I. Activity of Kaempferia pandurata (Roxb.) Rhizome Ethanol Extract Against MRSA, MRCNS, MSSA, Bacillus subtilis and Salmonella typhi. Pakistan Journal of Biological Science 2014; 17(1): 49-55.

Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pacific Journal of Tropical Biomedicine. 2012;2(8):597-601.

Sichani M and Karbasizadeh M. Effect of different extracts of Stevia rebaudiana leaves on Streptococcus mutans growth. Journal of Medicinal Plants Research. 2012;6(32) : 4731-4734.

Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochem Anal Biochem 2013; 2: 144.

Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. Journal of Advanced Pharmaceutical Technology & Research 2011;2(2):104-109.

Sen A and Batra A. Evaluation Of Antimicrobial Activity Of Different Solvent Extracts Of Medicinal Plant: Melia Azedarach L. International Journal of Current Pharmaceutical Research 2012 ; 4(2): 67-73.

Clinical and Laboratory Standards Intitute, 2009, M07-A08 – Methods Dilution Antimicrobial Succeptibility Tests for Bacteria That Grow Aerobically; Approve Standard, 8th Edition, CLSI, Pennsylvania.

Meletiadis J, Pournaras S, Roilides E, and Walsh TJ. Defining Fractional Inhibitory Concentration Index Cutoffs for Additive Interactions Based on Self-Drug Additive Combinations, Monte Carlo Simulation Analysis, and In Vitro-In Vivo Correlation Data for Antifungal Drug Combinations against Aspergillus fumigatus. Antimicrob. Agents Chemother. 2010 ; 54 (2) : 602-609.

Doughari J, Okafor B. Antimicrobial Activity of Senna alata Linn, East and Central African Journal of Pharmaceutical Sciences 2007, 10: 17-21.

Najafi S. Phytochemical screening and antibacterial activity of leaf Extract of Ziziphus Mauritiana Lam. International Research Journal of Applied and Basic Sciences 2013, 4 (11): 3274-3276.

Kumar S, Pandey AK. Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal 2013

Norajit, K, Laohakunjit, N, Kerdchoechuen, O. Antibacterial effect of five Zingiberaceae essential oils. In:Chahyadi A, Hartati R, Wirasutisna KR, Elfahmi. Boesenbergia pandurata Roxb., An Indonesian Medicinal Plant: Phytochemistry, Biological Activity, Plant Biotechnology. Procedia Chemistry 2014; 13, p.13-37..

Phongpaichit S, Subhadhirasakul S, and Wattanapiromsakul C.Antifungal activities of extracts from Thai medicinal plants against opportunistic fungal pathogens associated with AIDS patients. In: Chahyadi A, Hartati R, Wirasutisna KR, Elfahmi. Boesenbergia pandurata Roxb., An Indonesian Medicinal Plant: Phytochemistry, Biological Activity, Plant Biotechnology. Procedia Chemistry 2014; 13, p.13-37.

Giriraju A and Yunus GY. Assessment of antimicrobial potential of 10% ginger extract against Streptococcus mutans, Candida albicans, and Enterococcus faecalis: An in vitro study, Indian J Dent Res. 2013;24(4):397-400

Adwan G and Mhanna M. Synergistic effect of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Asian Pacific Journal Tropical Medicine 2009; 2(3): 46-51.

Rakholiya K and Chanda S. In vitro interaction of certain antimicrobial agents in combination withplant extracts against some pathogenic bacterial strains. Asian Pacific Journal of Tropical Biomedicine 2012:S876-S880.

Olajuyigbe OO and Afolayan AJ. Evaluation of Combination Effects of Ethanolic Extract of Ziziphus mucronata Willd. subsp. mucronataWilld. and Antibiotics against Clinically Important Bacteria. The ScientificWorld Journal 2013, 1-9.

Pinho MG, Lencastre H, and Tomasz A. An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. In Chovanova R, Mikulasova, Veverkova S. In Vitro Antibacterial and Antibiotic Resistance Modifying Effect of Bioactive Plant Extracts on Methicillin-Resistant Staphylococcus epidermidis. International Journal of Microbiology 2013:1-7.

Published

01-01-2016

How to Cite

Sukandar, E. Y., N. F. Kurniati, P. Wikaningtyas, and D. Agprikani. “ANTIBACTERIAL INTERACTION OF COMBINATION OF ETHANOLIC EXTRACT OF ZINGIBER OFFICINALE VAR RUBRUM RHIZOME, BOESENBERGIA PANDURATA RHIZOME, AND STEVIA REBAUDIANA LEAVES WITH CERTAIN ANTIBIOTICS AGAINST INFECTIOUS MOUTH MICROBIAL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 1, Jan. 2016, pp. 332-5, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/10059.

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 > >>