A PREVENTIVE APPLICATION IMPROVIZED USING ARID PLANT DERIVATES: NOSOCOMIAL PREVENTIVE REMEDY
Abstract
ABSTRACT
Objective: Epidermis acts as a niche for plethora of infection causing bacteria. It is a necessity to maintain the microbial populations in acceptable
levels to attain hygienic conditions. In this study, an attempt has been made to prepare and test the functioning of antimicrobial cloth using desert
plant extracts.
Methods: Comparative antibacterial and radical scavenging activity of the aqueous and methanolic extracts from plants namely Saccharum
spontaneum, Prosopsis cineria and Balanites aegyptica was tested. Minimum inhibitory concentration (MIC) against Staphylococcus aureus MTCC 7443,
Streptococcus pneumonia MTCC 655, Escherichia coli NCIM 2642, Pseudomonas aeroginosa MTCC 8295, Bacillus megaterium NCIM 2326, Bacillus
subtilis NCIM 2329 ranged from 20 µg to 50 µg for the various extracts utilized. DNAse activity was also checked for the extracts to estimate the
potency of extract for skin damage.
Results: Based on the MIC ranges, an excess amount (100 µg) of the extract was loaded on the cotton cloth and check for antimicrobial potential. DNAse
activity was also checked for the extracts to estimate the potency of extract for skin damage. No extract tested was found to have DNA degradation
activity.
Conclusion: Based on the manifested results, cotton cloth loaded with 100 µg/cm
of crude extracts from Balanites aegyptica may be successfully
used as an antimicrobial cloth. Fabrics capable of sterilization would have potential benefits to reduce disease transfers among hospital populations
and bio‑warfare protection.
Keywords: Balanites aegyptica, Antimicrobial, Cotton cloth, Desert plant extract, Skin microflora.
2
Downloads
References
MacLachlan A. Body Bacteria: Exploring the Skin's Microbial Metropolis, FINDINGS National institute of general medical sciences, 2012; 1-9.
Nester E, Anderson, Jr. D, Roberts CE. Evans Microbiology: A Human Perspective [McGraw-Hill Higher Education, 2011]
Dellion S, Morel P, Vignon-Pennamen D, Felten A,. Erythrasma owing to an unusual pathogen. Arch Dermatol 1996;132: 716-717.
E P Baorto D. Baorto Staphylococcus Aureus Infection . Medscape Medical News.[http://www.medscape.com/viewarticle/971358]
Lynch JP. 3rd, G. G. Zhanel, Streptococcus pneumoniae: epidemiology and risk factors, evolution of antimicrobial resistance, and impact of vaccines. Curr Opin Pulm Med.,16[3],2010, 217-25.
Siegel JD, Rhinehart E, Jackson M, Chiarello L. HICPAC, Management of Multidrug-Resistant Organisms in Healthcare Settings 2006; [www.cdc.gov/ncidod/dhqp/pdf/ar/mdroGuideline2006.pdf]
Aly R Microbial Infections of Skin and Nails. Medical Microbiology, 4th edition. [University of Texas Medical Branch at Galveston; 1996]
Jia Y , Zhao G, Jia J. Preliminary evaluation: the effects of Aloe ferox Miller and Aloe arborescens Miller on wound healing. J Ethnopharmacol.2008; 120:181-189.
Carson CF, Hammer KA, Riley TV Melaleuca alternifolia [Tea Tree] Oil: a Review of Antimicrobial and Other Medicinal Properties, Clin Microbiol Rev 2006;19:50–621.
Ahmad M, Ghafoor N, Aamir MN. Antibacterial activity of mother tinctures of cholistan desert plants in Pakistan. Indian J Pharm Sci 2012;74:465-468.
McKay DL, Blumberg JB. A review of the bioactivity of South African herbal teas: rooibos [Aspalathus linearis] and honeybush [Cyclopia intermedia]. Phytother Res, 2007; 21: 1-16.
Said O, Khalil K, Fulder S , Azaizeh H. Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region, Journal of Ethnopharmacology 2002;83: 251-265.
Suleiman MN The in vitro phytochemical investigation on five medicinal plants in Anyigba and its environs, Kogi State, Nigeria. Der Pharmacia Sinica 2011;2:108-111.
Joshi N, Bhatt S, Dhyani S, Nain J. Phytochemical screening of secondary metabolites of Argemone mexicana linn. flowers. Int J Curr Pharm Res 2013; 5:144-147.
Treare GE, Evans WC. Pharmacognosy, 17th edn, [Bahiv Tinal, London, 1985].
Kokate CK, Practical Pharmacognosy. 4th Edn. [Vallabh Prakashan, 1994]
Khandelwal KR [2004]. Practical Pharmacognosy, Techniques and experiments [12th Edn]. Nirali Prakashan, Pune India. 157
Ghani A Medicinal plants of Bangladesh. Chemical constituents and uses. Dhaka: The Asiatic Society of Bangladesh 2003;500–508.
Geethadevi R , Maheshwari V. Imparting Antimicrobial Finish on Selected Regenerated Cellulosic Fabrics with Herbal Oil Combinations. J Textile Sci Eng 2013; 4:144. doi: 10.4172/2165-8064.1000144
Song C,Yu H, Zhang M, Yang Y, Zhang G Int J Biol Macromol 2013;60:347-54. doi: 10.1016/j.ijbiomac.2013.05.039.
Subhashini N, Nagarajan G, Kavimani S. In Vitro Antioxidant And Anticholinesterase Activities Of Garcinia Combogia Int J Pharm Pharm Sci 2011;3:129Â132.
Vedak S, Raut SV. Study on Antibacterial Compoundsfrom Methanolic Extract of Bark of Prosopis juliflora [Vilayati babhul] International Journal of Pharmaceutical Sciences and Business Management 2014; 2:1-14.
Sathiya M , Muthuchelian K. Investigation of Phytochemical andantibacterial potential of ethanolic leaf extract of Prosopis julilfora DC.Ethanolbaotonical leaflets. 2008;12:1240-1245.
Ahmad A, Khan KA, Ahmad VU, Qazi S Antibacterial activity of juliflorine isolated from Prosopis juliflora. Planta Med, 1, 1986; 285-288.
Park J, Kaufmann GF, Bowen JP, Arbiser JL , Janda KD. Solenopsin A. A venom alkaloid from the fire ant Solenopsis invicta, inhibits quorum-sensing signaling in Pseudomonas aeruginosa. J Infect Dis, 2008; 198:1198-201.
Kahsay T, Mulugeta A Unnithan CR. Antioxidant and Antibacterial Activities of Balanites aegyptiaca delil from Northern Ethiopia, Am. J. PharmTech Res., 4[3], 2014, 415-422.
Shahid M, Jahan N, Shahzad A, Sahai A, Sharma S, Parveen S, Antimicrobial Potential of Balanites Aegyptiaca [L.] Del, Stevia Rebaudiana [Bert.] Bertoni, Tylophora Indica [Burm.f.] Merrill, and Cassia Sophera [Linn.] ,The Open Conference Proccedings Journal 2012;3:63-69.
Smith AH, Imlay JA, Mackie RI Increasing the Oxidative Stress Response Allows Escherichia coli To Overcome Inhibitory Effects of Condensed Tannins, Appl Environ Microbiol 2003;69:3406–3411.
Sen S , Chakraborty R. The Role of Antioxidants in Human Health, Oxidative Stress: Diagnostics, Prevention, and Therapy, ACS Symposium Series, 201;1-37.
Olila D, Opuda-Asibo J, Odyek O. Bioassay-guided studies on the cytotoxic and in vitro trypanocidal activities of a sesquiterpene [Muzigadial] derived from a Ugandan medicinal plant [Warburgia ugandensis], Afr Health Sci. 2001;1:12–15.
Robbins PJ , Cherniack MB Review of the biodistribution and toxicity of the insect repellent N,N-diethyl-m-toluamide [DEET]. J Toxicol Environ Health 1986;18:502-525.
Feldmann RJ, Maibach HI Absorption of some organic compounds through the skin in man. J Invest Derm 1970;54:399-404.
Al-Ghannam SM, Ahmed HH, Zein N, Zahran F. Antitumor Activity of Balanitoside Extracted from Balanites aegyptiaca Fruit, Journal of Applied Pharmaceutical Science2013;3:179-191.
Imlay J, Fridovich I. Exogenous quinones directly inhibit the respiratory NADH dehydrogenase in Escherichia coli Arch Biochem Biophys 1992; 296:337-346.
Ripa FA, Haque M, Imran-Ul-Haque M. In Vitro Antimicrobial, Cytotoxic and Antioxidant Activity of Flower Extract of Saccharum Spontaneum Linn. Eur. J. Scien. Res. 2009; 30:478.
Hartwell JL. Plants used against cancer:A survey, Lloydia, 1971: 30-34.
Swapnil SS, Verma SK. Antibacterial properties of Alkaloid rich fractions obtained from various parts of Prosopis juliflora IJPSR 2011; 2:114-120.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.