MODIFIED BIPHASIC MEDIA FOR BLOOD CULTURE

Authors

  • Bhuvaneshwar Devaraj Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.
  • Arunava Kali Assistant Professor, Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry.
  • Pravin Charles M.v. Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.
  • Kunigal S Seetha Professor and Head, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Abstract

ABSTRACT
Objective: To evaluate the performance characteristics of Nutrient agar and MacConkey's agar biphasic medium (BPM) with conventional blood
culture.
Methods: We prepared two modified biphasic blood culture medium with Nutrient agar (with 1% lactose and 0.004% neutral red) and MacConkey's
agar. A total of 20 standard clinical strains of bacterial pathogens and Candida albicans were inoculated in duplicate in Nutrient agar BPM, MacConkey's
agar, and brain heart infusion broth to evaluate the turn-around time and performance characteristics.
Results: All isolates except Streptococcus pyogenes and Streptococcus pneumoniae showed characteristic lactose fermenter and non-lactose fermenter
colonies on Nutrient agar and MacConkey's agar BPM within 24 hrs with sufficient growth to carry out biochemical identification and antibiotic
susceptibility testing. This resulted in a shorter turn-around time (48 hrs) when compared to that of conventional blood culture (72 hrs).
Conclusion: Nutrient agar and MacConkey's agar based modified BPM could be beneficial in reducing the turn-around time of blood culture.
Keywords: Biphasic media, Bloodstream infection, Conventional blood culture.

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Author Biographies

Bhuvaneshwar Devaraj, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Arunava Kali, Assistant Professor, Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry.

Assistant Professor, Microbiology,
Mahatma Gandhi Medical College and Research Institute, Pondicherry.

Pravin Charles M.v., Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Assistant Professor, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Kunigal S Seetha, Professor and Head, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

Professor and Head, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry-607402, India.

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Published

01-05-2016

How to Cite

Devaraj, B., A. Kali, P. Charles M.v., and K. S. Seetha. “MODIFIED BIPHASIC MEDIA FOR BLOOD CULTURE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 3, May 2016, pp. 42-43, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/10220.

Issue

Vol 9 Issue 3 (May-June) 2016

Section

Short Communication(s)

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