EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF OCIMUM BASILICUM AND MENTHA SPICATA EXTRACTS
Abstract
Objective: The objective of the current research was to evaluate the cytotoxicity of Ocimum basilicum (OB) and Mentha spicata (MS).
Methods: In vitro cytotoxic potential of OB and MS extracts was evaluated against human cancer HL60 cell lines using 3-[4,5-dimethylthiazol-2-yl]-2,5
diphenyl tetrazolium bromide assay. The treated cell morphology was observed by light microscope.
Results: The OB and MS extracts inhibited proliferation of HL60 cells in concentration dependent manner with IC₅₀ of 45.67 µg/ml and 98.1 µg/ml
for 42 hrs, respectively. The morphology of the HL60 cells treated with OB and MS extracts at high concentrations (200 and 100 µg/ml) indicated cell
shrinkage, clumping, and signs of apoptosis.
Conclusion: OB and MS extracts induced cell death in HL60 cell lines. Further, in vivo studies and identification of active components from OB extract,
and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.
Keywords: Cytotoxicity; HL60 cell; apoptosis; medicinal plant.
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