IN-VITRO PHYTOCHEMICAL ANALYSIS, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI PESTALOTIOPSIS SP. ISOLATED FROM ACALYPHA INDICA (LINN)
Abstract
ABSTRACT
Objective: This study deals with phytochemical analysis, high-performance liquid chromatography (HPLC), antimicrobial activity analysis of
endophytic fungi Pestalotiopsis sp. isolated from midrib and lamina region of Acalypha indica leaves.
Methods: An endophytic fungus isolated from potato dextrose agar medium. Ethyl acetate extracts of Pestalotiopsis sp. were investigated for their
HPLC, and a qualitative analysis of the phytochemical analysis was performed. The antibacterial activity of isolated endophytic fungi was tested
against two Gram-positive bacteria (Staphylococcus aureus and Streptococcus pneumoniae) and two Gram-negative bacteria (Salmonella typhi and
Escherichia coli) by disk diffusion method.
Results: The crude extract of Pestalotiopsis sp. HPLC retention time of 5.76 shows the maximum area of 78.15%. The qualitative analysis of endophytic
fungi showed positive results for terpenoid and negative results for alkaloid, flavanoid, tannin, and carbohydrate. Crude extracts could inhibit Grampositive
bacteria
(S.
aureus
and
S. pneumoniae)
and Gram-negative
bacteria
(S.
typhi
and
E. coli).
Conclusion: This study recorded that Pestalotiopsis sp. produce bioactive compound. Further studies on characterization, in-vitro and in-vivo
studies, would reveal the antibacterial activity can be performed to see the activity of this endophytic fungus in clinical trials against different
human ailments.
Keywords: Pestalotiopsis sp., Phytochemical analysis, Antibacterial activity, Acalypha indica.
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References
REFERENCES
Petrini O, Sieber TN, Toti L, Viret O. Ecology, metabolite
production, and substrate utilization in endophytic fungi. Nat Toxins
;1(3):185-96.
Caroll CG. Fungal endophytes in stem and leaves from latent pathogent
mutualistic symbiont. Ecology 1988;69(1):2-9.
Li JY, Strobel G, Sidhu R, Hess WM, Ford EJ. Endophytic taxolproducing
fungi from bald cypress, Taxodium
distichum.
Microbiology
;142:2223-6.
Li JY, Strobel GA. Jesterone and hydroxy-jesterone antioomycete
cyclohexenone epoxides from the endophytic fungus Pestalotiopsis
jesteri. Phytochemistry 2001;57(2):261-5.
Ong HC, Noralina J. Malay herbal medicine in Gemencheh, Negeri
Sembilan, Malaysia. Fitoterapia 1998;70(1):10-4.
Dobranic JK, Johnson JA, Alikhan QR. Isolation of endophytic fungi
from Eastern larch (Larix laricina) leaves from New Brunswick,
Canada. Can J Microbiol 1995;41:194-8.
Bills GF, Polishook JD. Recovery of endophytic fungi from
Chamaecyparis thyoides. Sydowia 1992;44:1-12.
Ellis MB. Dematiaceous Hyphomycetes. Kew, Surrey, England:
Commonwealth Mycological Institute; 1971.
Sutton BC. In: Answorth BC, Sparrow FK, Sussman AS. The Fungi.
Vol. 4A. London: Academic Press; 1973. p. 513-82.
Suthep W, Nongluksna A, Nuntawan T, Kannawat D, Nijsiri R,
Rithaya M. Endophytic fungi with anti-microbial, anti-cancer and
anti-malarial activities isolated from Thai medicinal plants. World J
Microbiol Biotechol 2004;20:265-72.
Cardellina JH. HPLC separartion of taxol and cephalomannine.
J Chromatogr 1991;14(1):659-65.
Becerro MA, Lopez NI, Turon X, Uniz MJ. Antimicrobial activity
and surface bacterial film in marine sponges. J Exp Mar Biol Ecol
;179:195-205.
Suryanarayanan TS, Senthilarasu G, Muruganandam V. Endophytic
fungi from Cuscuta reflexa and its host plants. Fungal Divers
;4:117-23.
Suryanarayanan TS, Thennarasan S. Temporal variation in endophytes
assemblages of Plumeria rubra leaves. Fungal Diver 2004;15:197-204.
Cabral D, Stone JK, Caroll GC. The internal mycoflora of Juncus sp.:
Microscorpic and cultured observation of infection patterns. Mycol Res
:97:367.
Kathiravan G, Muthumary J. Extraction of taxol, an anticancer drug
from coelomycetous fungi Pestalotiopsis versicolor and Phyllosticta
murraicola. Myco Balcanica 2009;6:55-60.
Xu L, Zhou L, Zhao J, Jiang W. Recent studies on the antimicrobial
compounds produced by plant endophytic fungi. Nat Prod Res Dev
;20(4):731-40.
Maria GL, Sridhar KR, Raviraja NS. Antimicrobial and enzyme activity
of mangrove endophytic fungi of Southwest coast of India. J Agric
Technol 2005;1:67-80.
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