IN-VITRO PHYTOCHEMICAL ANALYSIS, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI PESTALOTIOPSIS SP. ISOLATED FROM ACALYPHA INDICA (LINN)

Authors

  • SOWPARTHANI K Valliammal college for womenMadras university,chennai

Abstract

ABSTRACT
Objective: This study deals with phytochemical analysis, high-performance liquid chromatography (HPLC), antimicrobial activity analysis of
endophytic fungi Pestalotiopsis sp. isolated from midrib and lamina region of Acalypha indica leaves.
Methods: An endophytic fungus isolated from potato dextrose agar medium. Ethyl acetate extracts of Pestalotiopsis sp. were investigated for their
HPLC, and a qualitative analysis of the phytochemical analysis was performed. The antibacterial activity of isolated endophytic fungi was tested
against two Gram-positive bacteria (Staphylococcus aureus and Streptococcus pneumoniae) and two Gram-negative bacteria (Salmonella typhi and
Escherichia coli) by disk diffusion method.
Results: The crude extract of Pestalotiopsis sp. HPLC retention time of 5.76 shows the maximum area of 78.15%. The qualitative analysis of endophytic
fungi showed positive results for terpenoid and negative results for alkaloid, flavanoid, tannin, and carbohydrate. Crude extracts could inhibit Grampositive
bacteria
(S.
aureus
and
S. pneumoniae)
and Gram-negative
bacteria
(S.
typhi
and
E. coli).
Conclusion: This study recorded that Pestalotiopsis sp. produce bioactive compound. Further studies on characterization, in-vitro and in-vivo
studies, would reveal the antibacterial activity can be performed to see the activity of this endophytic fungus in clinical trials against different
human ailments.
Keywords: Pestalotiopsis sp., Phytochemical analysis, Antibacterial activity, Acalypha indica.

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References

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Published

01-07-2016

How to Cite

SOWPARTHANI K. “IN-VITRO PHYTOCHEMICAL ANALYSIS, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI PESTALOTIOPSIS SP. ISOLATED FROM ACALYPHA INDICA (LINN)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 101-3, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/11577.

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