ANTIMICROBIAL AND PHYTOCHEMICAL ANALYSIS OF LEAF EXTRACT OF MEDICINAL FRUIT PLANTS
Abstract
Objective: The present study objective was to describe the in-vitro antimicrobial and antifungal activity of ethyl acetate extracts from leaves of papaya, pomegranate, banana and guava. , Present investigation showed that leaves extract of fruits plants are good source of bioactive compounds which have some ethnomedicinal applications were screened for their antibacterial activity against bacterial pathogen of human.
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Methods: A total of four plant extracts were used in this study to examine their antimicrobial properties and phytochemical analysis. Antimicrobial activity was evaluated for crude ethyl acetate extracts against human pathogen Escherichia coli, Bacillus subtilis, Staphylococcus  aureus and Pseudomonas aeruginasa using an agar diffusion assay. Phytochemical analysis tests for the screening and identification of bioactive chemical constituents of extracts of the fruits leaf was performed. This study was also conducted to determine the total phenols present in leaf extract of fruit plants.Results : The guava leaves crude extract showed MIC of 3.75 mg/ml for B. subtilis and P. aeruginasa, which showed its efficacy as a potent antimicrobial. Phytochemical analysis of the extracts revealed the presence of bioactive compound such as saponins, alakaloids, flavonoids, terpenoids, carbohydrates and tannins. The ethyl acetate extract of banana produced highest zone of inhibition 23 mm for B subtilis. This study showed that Punica granatum leaf is a good source of phenolic compounds.
Conclusion: This study concludes that these fruit leaves are potential source for bioactive metabolites and may be used in Pharmaceutical industry. On the basis of the present finding leaf extract of fruits possess the capabilities of being a good candidate in the search for a natural antimicrobial agent against infections and/or diseases caused by human pathogens.
Key words:Â Leaves extract, Fruits, Antimicrobial activities, Antifungal activities, Minimum inhibitory concentration, Phytochemicals analysis
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Levy SB, Marshall B. Antibacterial resistance worldwide: causes, challenges and responses. Nature Med 2004; 10: 122-129.
Ibrahim SA, Salameh MM, Phetsomphou S, Winstanley H, Limb TG, et al. Ten year survey of the antimicrobial susceptibility of urinary tract isolates in the U K: The Micro based Project. J Antimicr Chemother 1997; 40: 591-594.
Ibrahim SA, Salameh MM, Phetsomphou S, Yang H, Seo CW. Application of caffeine, 1,3,7-trimethylxanthine, to control Escherichia coli O157:H7. Food Chem 2006; 99: 645-650.
Kim S, Fung DYC. Antibacterial effect of crude water-soluble arrowroot (Puerariae radix) tea extracts on food-borne pathogens in liquid medium. Lett Appl Microbiol 2004; 39:319-325.
Alam MT, Karim MM, Khan SN. Antibacterial activity of different organic extracts of Achyranthes aspera and Cassia alata. J Sci Res 2009; 1: 393-398.
Iyengar, M.A. Study of crude drugs. College of Pharmaceutical Sciences, Manipal 1985; 2: 13-78.
Nicholson RL, Hammerschmidt R. Phenolic compounds and their role in disease resistance. Annu Rev Phytopathol 1992; 30: 369-389.
Grosvenor PW, Supriono A, Gray DO. Medicinal plants from Riau Province, Sumatra, Indonesia. Part 2, Antibacterial and antifungal activity. J. Ethnopharmacol 1995; 45: 97-111.
Murugesan S, Pannerselvam A, Tangavelou AC. Phytochemical screening and antimicrobial activity of the leaves of Memecylon umbellatum burm. F. J Pharm Sci 2011; 1: 42-45.
Chu HL, Yeh DB, Shaw JF. Production of L-DOPA by banana leaf polyphenol oxidase. Bot Bull Acad Sin 1993; 34: 57- 60.
Swarnamoni D, Barman S. Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats. Indian J Pharmacol 2012; 44(2): 219.
Burkill HM. The useful plants of west tropical Africa. Royal Botanical Garden Kew 1985; (7)1: 200- 219.
Morton J, Miami FL. In: Fruits of warm climates. 1987; 356- 363.
Mukhtar MD, Tukur A. In vitro screening for activity of Pistia stratiotes extracts. Niger Soc for Exp Biol J 1999; 1 (1): 51-60.
Agarwal PK, Agarwal S. In vitro antibacterial activity of methanolic extract from some herbal plants. J Biol Sci Opin 2015; 3(3): 128-132.
Singh J, Tripathi NN. Inhibition of storage fungi of blackgram (Vigna mungo) by some essential oils. Flav and Frag J 1999; 14: 1-4.
Devi NN, Prabakaran JJ, Wahab F. Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica. Asian Pacific J Trop Biomed 2012; 2: 1280-1284.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substances by means of Folin-Ciocalteu reagent. Methods Enzymol 1999; 299: 152-178.
Silici S, Sagdic O, Ekici L. Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys. Food chem 2010; 121(1): 238-243.
S. O. Gnan, M. T. Demello. Inhibition of Staphylococcus aureus by aqueous Goiaba extracts. J. Ethnopharmacology 1999; 68:103-108.
Qadan F A, Thewaini A, AliDA, Afifi R, Elkhawad A, Matalka KZ. The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to acne-developing organisms. Am J Chin Med 2005; 33:197-204.
Mathabe MC, Nikolova RV, Lall N, Nyazema NZ. Antibacterial activities of medicinal plants used for the treatment of diarrhea in Limpopo Province, South Africa. J. Ethnopharmacol 2005; 105: 286-293.
Nair R, Kalariya T, Chhanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol. 2005;29: 41-47.
Nirosha, N. and Mangalanayaki, R. Antimicrobial activity of leaves and stem extract of Carica papaya L. IJAPBC 2013; 2(3):2277-4688.
Alisi CS, Nwanyanwu CE, Akujobi CO, Ibegbulem CO. Inhibition of dehydrogenase activity in pathogenic bacteria isolates by aqueous extracts of Musa paradisiaca (Var Sapientum). Afr J Biotechnol 2008; 7 (12): 1821-1825.
Kumar M, Dandapat S, Sinha MP. Phytochemical screening and antibacterial activity of aqueous leaf extract of Punica granatum. 2015, 6(3), XX-XX.
Bansode DS, Chavan MD. Screening of Guava (Psidium gaujava) for effective phytomedicines and study on its antimicrobial effect against selected enteric pathogens. IJAPBC 2014; 3(3): 802-805.
Bautista-Banos S, Hernandez-Lopez M, Bosquez-Molina E, Wilson CL. Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop Prot 2003; 22: 1087-1092.
Ahmed, I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethnopharmocol 2001; 74: 113.
Karsha, PV, Naseem, S, Bhagyalakshmi, O. Antimicrobial activity of leaf extracts of guava (Psidium guajava) and papaya (Carica papaya). Advancs in Plant Sci 2009; 22: 429-431.
Porwal, Vibha, Pallavi Singh, Devendra Gurjar. A comprehensive study on different methods of extraction from guaava leaves for curing various health problems. Int J Eng Res 2012; 2(6): 490-496.
Oliva A, Meepagala K, Wedge D, Harries D, Hale A, Aliotta G, Duke S. Natural fungicides from Ruta graveolens L. leaves, including a new quinolone alkaloid. J Agric Food Chem 2003; 51: 890-896.
Osawa, T. Novel natural antioxidants for utilization in food and biological systems. Postharvest Biochemistry of Plant Food-materials in the Tropics, Japan Scientific Press, Tokyo, Japan 1994; 241-251.
Reddy NS, Navanesan S, Sinniah SK, Wahab NA, Sim KS. Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complement Altern. Med. 2012; 12: 128-134.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centauea L. species. Food Chem. Toxicol 2013; 55: 290-296.
Sahaa KR , Acharyaa S , Shovon SSH, Royb P. Medicinal activities of the leaves of Musa sapientum var. sylvesteris in vitro. Asi Pac J Trop Biomed 2013; 3(6): 476-482.
Pushpangadan P, Kaur J, Sharma J. Plantain or edible banana (Musa x paradisica var. sapiemtum) some lesser known folk uses in India. Anc Sci Life 1989; 9: 20-24
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