PROTECTIVE EFFECT OF NON-IRRADIATED AND Γ-IRRADIATED MANGO (MANGIFERA INDICA L.) JUICE AGAINST ETHANOL-INDUCED OXIDATIVE STRESS IN ALBINO RATS
Abstract
ABSTRACT
Objective: The aim of this study is to explore the protective effect of non-irradiated and γ-irradiated mango juice (NMJ and GMJ) against ethanolinduced
hepatic and renal
dysfunction
in rats.
Methods: A total of 36 male Wistar rats were selected and randomly divided into six groups of six animals each, and the treatment was given every
day via orogastric tube for 30 days. Group I rats served as normal control group. Group II, III, IV, V, and VI rats received absolute ethanol, NMJ, GMJ,
ethanol+NMJ, and ethanol+GMJ, respectively. At the end of experimental period, the animals were sacrificed, and serum was separated for liver
and kidney markers analysis. The tissues (liver and kidney) were excised for histopathological changes and lipid peroxidation, enzymatic, and nonenzymatic
antioxidants
activities
were
estimated.
Results: Ethanol treatment resulted in the depletion of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-stransferase
activities,
and
reduced
vitamin
C
and
reduced
glutathione
content,
higher
level
of
malondialdehyde,
increased
activities
of
serum
alanine
aminotransferase,
aspartate
aminotransferase,
alkaline
phosphatase,
gamma-glutamyl
transpeptidase and elevated levels of serum urea, creatinine,
uric acid causing malfunction of hepatic, and renal tissues, when compared to control rats. Mango juice (both NMJ and GMJ) co-treatment with ethanol
ingested rats, were envisaged to possess significant antioxidant defense properties and help in the recovery of tissues from ethanol-induced oxidative
damage. The results showed that degenerative changes in hepatic and renal cells of ethanolic groups were minimized by the administration of mango
juice, as revealed by histopathological examination.
Conclusion: The current findings suggest that treatment with mango juice reduces ethanol-induced oxidative stress, thereby protecting the hepatic
and renal tissue from ethanol-induced damage.
Keywords: γ-irradiation, Mango juice, Ethanol, Oxidative stress, Antioxidant enzymes, Liver, Kidney.
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References
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