PROTECTIVE EFFECT OF NON-IRRADIATED AND Γ-IRRADIATED MANGO (MANGIFERA INDICA L.) JUICE AGAINST ETHANOL-INDUCED OXIDATIVE STRESS IN ALBINO RATS

Authors

  • Kondapalli Naresh Sri Venkateswara University Tirupati - 517 502, INDIA
  • Sadineni Varakumar Sri Venkateswara University Tirupati - 517 502, INDIA
  • Natava Rajesh Sri Venkateswara University Tirupati - 517 502, INDIA
  • Prasad Shekhar Variyar Bhabha Atomic Research Centre, Mumbai – 400 085, India
  • Obulam Vijaya Sarathi Reddy Sri Venkateswara University Tirupati - 517 502, INDIA

Abstract

ABSTRACT
Objective: The aim of this study is to explore the protective effect of non-irradiated and γ-irradiated mango juice (NMJ and GMJ) against ethanolinduced
hepatic and renal
dysfunction
in rats.
Methods: A total of 36 male Wistar rats were selected and randomly divided into six groups of six animals each, and the treatment was given every
day via orogastric tube for 30 days. Group I rats served as normal control group. Group II, III, IV, V, and VI rats received absolute ethanol, NMJ, GMJ,
ethanol+NMJ, and ethanol+GMJ, respectively. At the end of experimental period, the animals were sacrificed, and serum was separated for liver
and kidney markers analysis. The tissues (liver and kidney) were excised for histopathological changes and lipid peroxidation, enzymatic, and nonenzymatic
antioxidants
activities
were
estimated.
Results: Ethanol treatment resulted in the depletion of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-stransferase
activities,
and
reduced
vitamin
C
and
reduced
glutathione
content,
higher
level
of
malondialdehyde,
increased
activities
of
serum
alanine

aminotransferase,
aspartate
aminotransferase,
alkaline
phosphatase,
gamma-glutamyl

transpeptidase and elevated levels of serum urea, creatinine,
uric acid causing malfunction of hepatic, and renal tissues, when compared to control rats. Mango juice (both NMJ and GMJ) co-treatment with ethanol
ingested rats, were envisaged to possess significant antioxidant defense properties and help in the recovery of tissues from ethanol-induced oxidative
damage. The results showed that degenerative changes in hepatic and renal cells of ethanolic groups were minimized by the administration of mango
juice, as revealed by histopathological examination.
Conclusion: The current findings suggest that treatment with mango juice reduces ethanol-induced oxidative stress, thereby protecting the hepatic
and renal tissue from ethanol-induced damage.
Keywords: γ-irradiation, Mango juice, Ethanol, Oxidative stress, Antioxidant enzymes, Liver, Kidney.

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Author Biographies

Kondapalli Naresh, Sri Venkateswara University Tirupati - 517 502, INDIA

Department of Biochemistry Sri Venkateswara University Tirupati - 517 502, INDIA

Sadineni Varakumar, Sri Venkateswara University Tirupati - 517 502, INDIA

Department of Biochemistry Sri Venkateswara University Tirupati - 517 502, INDIA

Natava Rajesh, Sri Venkateswara University Tirupati - 517 502, INDIA

Department of Biochemistry Sri Venkateswara University Tirupati - 517 502, INDIA

Prasad Shekhar Variyar, Bhabha Atomic Research Centre, Mumbai – 400 085, India

Food Technology Division, Bhabha Atomic Research Centre, Mumbai – 400 085, India

Obulam Vijaya Sarathi Reddy, Sri Venkateswara University Tirupati - 517 502, INDIA

Prof.O.V.S.Reddy, Department of Biochemistry Sri Venkateswara University Tirupati - 517 502, INDIA Mobile:0091 94400 46293

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Published

01-07-2016

How to Cite

Kondapalli Naresh, Sadineni Varakumar, Natava Rajesh, P. S. Variyar, and Obulam Vijaya Sarathi Reddy. “PROTECTIVE EFFECT OF NON-IRRADIATED AND Γ-IRRADIATED MANGO (MANGIFERA INDICA L.) JUICE AGAINST ETHANOL-INDUCED OXIDATIVE STRESS IN ALBINO RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 147-55, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/11869.

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