IN VITRO MEROPENEM SUSCEPTIBILITY INDUCED BY PH ALTERATION IN METALLO-BETA-LACTAMASE POSITIVE PSEUDOMONAS AERUGINOSA
Abstract
ABSTRACT
Objective: The increasing emergence of metallo-beta-lactamase (MβL) producing Gram-negative bacteria such as Pseudomonas aeruginosa pose
a serious public health concern including in the treatment of urinary tract infection (UTI). This study was aimed to explore the combined effect of
in vitro pH alteration and antibiotic on the bacterial growth as a potential therapeutic approach against the drug resistant P. aeruginosa.
Methods: Ten MβL producing P. aeruginosa isolates from the patients suffering from UTI were included. Bacteria were inoculated with or without
meropenem in media with varied pH range from 5 to 10. The variation of bacterial growth was determined by measuring the changes of optical
density at 620 nm and colony forming unit counts/ml.
Result: The growth of bacteria was reduced both at very high and lower pH ranges. However, the growth was further reduced significantly with the
addition of meropenem at these extreme pH conditions.
Conclusion: Alteration of pH especially at lower range of the medium might has changed the efficacy of MβL and thus helped the antibiotic meropenem
to act on the bacteria, which was resistant toward the same at neutral pH. Combined effect of antibiotic and pH modulation of biological fluid like urine
should be explored for an effective alternative therapeutic approach against the drug-resistant bacteria.
Keywords: Metallo-beta-lactamase, urinary tract infection, Pseudomonas aeruginosa, pH.
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References
REFERENCES
Organization WH. Antimicrobial Resistance: Global Report on
Surveillance. Geneva: WHO Press; 2014.
National Nosocomial Infections Surveillance System. National
Nosocomial Infections Surveillance (NNIS) System Report, Data
Summary from January 1992 through June 2004, issued October 2004.
Am J Infect Control 2004;32(8):470-85.
Morrison AJ Jr, Wenzel RP. Epidemiology of infections due to
Pseudomonas aeruginosa. Rev Infect Dis 1984;6 Suppl 3:S627-4.
Mittal R, Aggarwal S, Sharma S, Chhibber S, Harjai K. Urinary tract
infections caused by Pseudomonas aeruginosa: A minireview. J Infect
Public Health 2009;2(3):101-11.
Sharma M, Yadav S, Chaudhary U. Metallo-beta-lactamase producing
Pseudomonas aeruginosa in neonatal septicemia. J Lab Physicians
;2(1):14-6.
Pallett A, Hand K. Complicated urinary tract infections: Practical
solutions for the treatment of multiresistant Gram-negative bacteria.
J Antimicrob Chemother 2010;65 Suppl 3:iii25-33.
Aiyegoro OA, Okoh AI. Use of bioactive plant products in combination
with standard antibiotics: Implications in antimicrobial chemotherapy.
J Med Plants Res 2009;3(13):1147-52.
O’Hara K, Haruta S, Sawai T, Tsunoda M, Iyobe S. Novel metallo betalactamase
mediated
by
a Shigella
flexneri
plasmid. FEMS
Microbiol
Lett
;162(2):201-6.
Clinical Laboratory Standards Institute. CLSI document M100-S20.
Performance Standards for Antimicrobial Susceptibility Testing.
Twentieth Informational Supplement. Wayne, PA: CLSI; 2010.
Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y. ImipenemEDTA
disk method
for differentiation
of metallo-beta-lactamaseproducing
clinical isolates
of Pseudomonas spp. and Acinetobacter
spp.
J
Clin
Microbiol 2002;40(10):3798-801.
Falagas ME, McDermott L, Snydman DR. Effect of pH on in vitro
antimicrobial susceptibility of the Bacteroides fragilis group.
Antimicrob Agents Chemother 1997;41(9):2047-9.
Danby CS, Boikov D, Rautemaa-Richardson R, Sobel JD. Effect of pH
on in vitro susceptibility of Candida glabrata and Candida albicans
to 11 antifungal agents and implications for clinical use. Antimicrob
Agents Chemother 2012;56(3):1403-6.
Rose C, Parker A, Jefferson B, Cartmell E. The characterization of
feces and Urine: A review of the literature to inform advanced treatment
technology. Crit Rev Environ Sci Technol 2015;45(17):1827-79.
Axelrod DR. Ascorbic acid and urinary pH. JAMA 1985;254(10):1310-1.
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