GAS CHROMATOGRAPHY-MASS SPECTROMETRIC ANALYSIS OF METHANOLIC LEAF EXTRACTS OF LANNEA KERSTINGII AND NAUCLEA DIDERRICHII, TWO MEDICINAL PLANTS USED FOR THE TREATMENT OF GASTROINTESTINAL TRACT INFECTIONS

Authors

  • Edewor Theresa Ibibia Ladoke Akintola University of Technology, Ogbomoso, Nigeria http://orcid.org/0000-0002-1362-3763
  • Kazeem Nimotalai Olabisi
  • OWA STEPHEN OLUWAGBEMIGA

Abstract

ABSTRACT Objective: The leaves of Lannea kerstingii and Nauclea diderrichii are traditionally used for the treatment of gastrointestinal infections. The aim of this work is to determine the phytochemicals that are present in the leaves. Methods: Phytochemical screening was carried out to determine the type of phyto-components present while a gas chromatography-mass spectrometry (GC-MS) analysis was to separate and identify the different phytochemicals that are present in the leaves. Results: The phytochemical screening showed the presence of tannins, flavonoids, saponins, and steroids in the methanolic extracts of both plants while only steroids were present in the n-hexane extract of N. diderrichii. Alkaloids, anthraquinone glycosides, and terpenoids were absent in all extracts. The GC-MS revealed 16 compounds in the methanolic extract of L. kerstingii with dodecanoic acid, methyl ester as the predominant compound (24.276%) and 26 compounds in N. diderrichii with phenol, 2,4-bis(1,1-dimethylethyl) - (17.253%) as the most abundant. Seven compounds were identified to be common in the two plants methanol extracts. Some of the identified compounds possess biological activities. Conclusion: Some of the identified compounds could be responsible for the biological activity of the plants leaves, especially its effect on gastrointestinal pathogens. Keywords: Lannea kerstingii, Nauclea diderrichii, Gastrointestinal infection, Gas chromatography-mass spectrometri.

Downloads

Download data is not yet available.

Author Biography

Edewor Theresa Ibibia, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

Department: Pure and Applied Chemistry

Rank: Associate Professor

References

REFERENCES

WHO. The World Health Report, Primary Health Care (Now More

Than Ever). Geneva: World Health Organization; 2008.

Malamud A, Wilson KT. Treatment of gastrointestinal infections. Curr

Opin Gastroenterol 2000;16(1):51-5.

Kalimuthu K, Prabakaran R. Preliminary phytochemical screening and

GC-MS analysis of methanolic extract of Ceropegia pusilla. Int J Res

Appl Nat Soc Sci 2013;1 Suppl 3:49-58.

Praveena A, Suriyavathana M. Phytochemical characterization of

Toddalia asiatica L Var. Floribunda stem. Asian J Pharm Clin Res

;6 Suppl 4:148-51.

Krishna S, Renu S. Isolation and identification of flavonoids from

Cyperis rotundus Linn. In vivo and in vitro. J Drug Deliv Ther

;3 Suppl 2:109-13.

Peter MP, Ray JY, Sicis VP, Joy V, Saravanan J, Sakthivel S. GC-MS

analysis of bioactive components on the leaves extract of Stylosanthes

fructicosa – Potential folklore medicinal plant. Asian J Plant Sci Res

;2 Suppl 3:243-53.

Gbile ZO, Soladoye MO. Vernacular Names of Nigerian Plants

(Yoruba). 2

ed. Ibadan: Forestry Research Institute of Nigeria; 2002.

Harborne JB. Phytochemical Methods, A Guide to Modern Techniques

nd

in Plant Analysis. New York: Chapman and Hall; 1993.

Dukes J. Phytochemical and Ethnobotanical Databases. Phytochemical

and Ethnobotanical Databases; 2013. Available from: http://www.arsgov/cgi-bin/duke/.

Thomas GA, Aneesh TP, Thomas DG, Anandan R. GC-MS analysis

of phytochemical compounds present in the rhizomes of Nervilia

aragoana. Asian J Pharm Clin Res 2013;6 Suppl 3:68-74.

Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italica

leaf methanol extract. Asian J Pharm Clin Res 2012;5(2):90-4.

Jayamathi T, Komalavalli N, Pandiyarajan V. GC-MS analysis of leaf

ethanolic extracts of Wrightia tinctoria - A high medicinal value plant.

Asian J Plant Sci Res 2012;2 Suppl 6:688-91.

Published

01-07-2016

How to Cite

Edewor Theresa Ibibia, K. Nimotalai Olabisi, and OWA STEPHEN OLUWAGBEMIGA. “GAS CHROMATOGRAPHY-MASS SPECTROMETRIC ANALYSIS OF METHANOLIC LEAF EXTRACTS OF LANNEA KERSTINGII AND NAUCLEA DIDERRICHII, TWO MEDICINAL PLANTS USED FOR THE TREATMENT OF GASTROINTESTINAL TRACT INFECTIONS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 179-82, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/11915.

Issue

Section

Original Article(s)