IN-VITRO HEPATOPROTECTIVE ACTIVITY OF ALBIZIA LEBBECK, CASSIA OCCIDENTALIS AND SWERTIA CHIRATA ON HEPG2 CELLS

Authors

  • Anil Kumar Dept. Of Biotechnology, Graphic Era University, Dehradun
  • Manju O Pai Department of Medical Microbiology & Medical Lab Technolgy, SBS PG Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India
  • Nishant Rai Dept. Of Biotechnology, Graphic Era University, Dehradun http://orcid.org/0000-0002-6108-2068

Abstract

ABSTRACT
Objective: The aim of this study was to investigate the in-vitro hepatoprotective activity of solvent extracts of Albizia lebbeck, Cassia occidentalis, and
Swertia chirata on HepG2 cell line.
Methods: The methanolic, ethanolic, and acetone seed extracts of A. lebbeck, C. occidentalis, and leaves extract of S. chirata were used in this study. The
different extracts of A. lebbeck, C. occidentalis, and S. chirata were assessed for their hepatoprotective activity on human liver hepatocellular carcinoma
(HepG2) cell line against paracetamol (PCM) as a liver damage inducing agent. The cell line viability was assessed by 3-(4,5-dimethyl thiazol-2-yl)-5diphenyltetrazolium
bromide
assay.
Results: The percentage cell viability was determined with respect to the normal control cells. Control cells showed 100% cell viability in all tested
plant extracts. The PCM treated HepG2 cells showed a maximum cell viability (46.6±2.49%) in presence of all seed extracts of A. lebbeck. The silymarin
and PCM treated HepG2 cells showed maximum cell viability (156.6±2.49%) presence of leaves extract of S. chirata. The maximum cells viability of
131.6±9.39% was observed in methanolic seed extract of A. lebbeck (50 µg/mL), and the minimum cell viability of 107.3±3.68% was observed in
acetone seed extract of C. occidentalis (50 µg/mL) comparatively.
Conclusions: The methanolic, ethanolic, and acetone extracts of seeds/leaves from A. lebbeck, C. occidentalis, and S. chirata were showed the
hepatoprotective activity. Further in vivo and clinical studies are required to confirm their therapeutic efficacy.
Keywords: Albizia lebbeck, Cassia occidentalis, Swertia chirata, Paracetamol, HepG2, [3-(4,5-dimethyl thiazol-2-yl)-5-diphenyltetrazolium bromide]
assay.

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Author Biographies

Anil Kumar, Dept. Of Biotechnology, Graphic Era University, Dehradun

Research Scholar,Dept. of Biotechnology

Manju O Pai, Department of Medical Microbiology & Medical Lab Technolgy, SBS PG Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India

Asst. Prof. Department of Medical Microbiology & Medical Lab Technolgy

Nishant Rai, Dept. Of Biotechnology, Graphic Era University, Dehradun

Assoc. Prof. Dept. Of Biotechnology

References

REFERENCES

Agarwal SS, Paridhavi M. Herbal Drug Technology. Hyderabad:

Universities Press Pvt., Limited; 2007. p. 123-32.

Saha A, Ahmed M. The analgesic and anti-inflammatory activities

of the extract of Albizia lebbeck in animal model. Pak J Pharm Sci

;22(1):74-7.

Chulet R, Joseph L, George M, Pradhan P. Pharmacognostic

standardization and phytochemical screening of Albizzia lebbeck.

J Chem Pharm Res 2010;2(1):432-43.

Chaddha V, Nayak S, Solanki S. Preliminary phytochemical screening

on bark and pods of Albizia lebbeck Linn. Int J Compr Pharm

;11(06):117-29.

Asian J Pharm Clin Res, Vol 9, Issue 4, 2016, 276-280

Kumar et al.

Khalid A, Amir W, Malik S, Ur-Rehman U, Sonia KA, Hassham HB.

Alternative medicine news. Health News Posted 2011;12(6):267-78.

Mahmood S, Hussain S, Tabassum S, Malik F, Riaz H. Comparative

phytochemical, hepatoprotective and antioxidant activities of various

samples of Swertia Chirayita collected from various cities of Pakistan.

Pak J Pharm Sci 2014;27(6):1975-83.

Verma H, Patil PR, Kolhapure RM, Gopalkrishna V. Antiviral activity

of the Indian medicinal plant extract, Swertia Chirata against herpes

simplex viruses. Indian J Med Microbiol 2008;26(4):322-6.

Prabakaran M, Chandrakala N, Panneerselvam A. Antimicrobial activity

of Indigofera glandulosa (wild). Asian J Plant Sci Res 2011;1(2):18-25.

Sheela JD, Jeeva IM, Shamila R, Lekshmi NC, Brindha JR.

Antimicrobial activity and phytochemical analysis of Sanseiveria

roxburghiana leaf. Asian J Plant Sci Res 2012;2(1):41-4.

Prema R, Sekar DS, Sekhar KB, Jeevanandham S. In vitro cytotoxicity

study on combined plants extracts (Cissus quadrangularis and Aegle

marmelos). Eur J Exp Biol 2012;2(4):882-8.

Saha P, Das S. Highlighting the anti-carcinogenic potential of an

Ayurvedic medicinal plant, Swertia Chirata. Asian Pac J Cancer Prev

;11(6):1445-9.

Verma VK, Khomendra K, Sarwa AK. Comparison of hepatoprotective

activity of Swertia Chirayita and Andrographis paniculata plant of

North-East India against CCl

induced hepatotoxic rats. J Pharm Res

;6-7(7):647-53.

Anand J, Gautam P, Rai N. Comparative study of antibacterial and

antiproliferative potential of green tea from different geographical

locations in India. Asian J Pharm Clin Res 2015;8(1):253-8.

Nath R, Roy S, De B, Choudhury MD. Anticancer and antioxidant

activity of Croton: A review. Int J Pharm Pharm Sci 2013;5(2):63-70.

Sujatha V, Kokila K, Priyadharshini SD. Phytopharmacological

properties of Albizia species: A review. Int J Pharm Pharm Sci

;5(3):70-3.

Otang WM, Grierson DS, Ndip RN. Cytotoxicity of three South

African medicinal plants using the Chang liver cell line. Afr J Tradit

Complement Altern Med 2014 28;11(2):324-9.

Akhir NA, Chua LS, Majid FA, Sarmidi MR. Cytotoxicity of aqueous

and ethanolic extracts of Ficus deltoidea on human ovarian carcinoma

cell line. Br J Med Med Res 2011;1(4):397-409.

Drever BD, Anderson WG, Riedel G, Kim DH, Ryu JH, Choi DY, et al.

The seed extract of Cassia obtusifolia offers neuroprotection to mouse

hippocampal cultures. J Pharmacol Sci 2008;107(4):380-92.

Mukherjee S, Sur A, Maiti BR. Hepatoprotective effect of Swertia

chirata on rat. Indian J Exp Biol 1997;35(4):384-8.

Sivakrishnan S, Kottaimuthu A. Hepatoprotective activity of ethanolic

extract of aerial parts of Albizia procera roxb (Benth.) Against

paracetamol induced liver toxicity on wistar rats. Int J Pharm Pharm

Sci 2014;6(1):233-8.

Shirode D, Roy SP, Patel T, Rajendra SV, Jyothi TM, Baganal P,

et al. Evaluation of hepatoprotective effect of leaves of seventy

percent ethanolic extract of Albizia lebbeck in paracetamol induced

experimental hepatic damage. Plant Arch 2008;8(2):797-801.

Kumar A, Rai N, Kumar N, Gautam P, Kumar JS. Mechanism involved

in hepatoprotection of different herbal products: Review. Int J Res

Pharm Sci 2013;4(1):1-6.

Kang J, Huo CH, Li Z, Li ZP. New ceramides from the flower of Albizia

julibrissin. Chin Chem Lettt 2007;18:181-4.

Published

01-07-2016

How to Cite

Kumar, A., M. O. Pai, and N. Rai. “IN-VITRO HEPATOPROTECTIVE ACTIVITY OF ALBIZIA LEBBECK, CASSIA OCCIDENTALIS AND SWERTIA CHIRATA ON HEPG2 CELLS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 276-80, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/12150.

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