IN-VITRO HEPATOPROTECTIVE ACTIVITY OF ALBIZIA LEBBECK, CASSIA OCCIDENTALIS AND SWERTIA CHIRATA ON HEPG2 CELLS
Abstract
ABSTRACT
Objective: The aim of this study was to investigate the in-vitro hepatoprotective activity of solvent extracts of Albizia lebbeck, Cassia occidentalis, and
Swertia chirata on HepG2 cell line.
Methods: The methanolic, ethanolic, and acetone seed extracts of A. lebbeck, C. occidentalis, and leaves extract of S. chirata were used in this study. The
different extracts of A. lebbeck, C. occidentalis, and S. chirata were assessed for their hepatoprotective activity on human liver hepatocellular carcinoma
(HepG2) cell line against paracetamol (PCM) as a liver damage inducing agent. The cell line viability was assessed by 3-(4,5-dimethyl thiazol-2-yl)-5diphenyltetrazolium
bromide
assay.
Results: The percentage cell viability was determined with respect to the normal control cells. Control cells showed 100% cell viability in all tested
plant extracts. The PCM treated HepG2 cells showed a maximum cell viability (46.6±2.49%) in presence of all seed extracts of A. lebbeck. The silymarin
and PCM treated HepG2 cells showed maximum cell viability (156.6±2.49%) presence of leaves extract of S. chirata. The maximum cells viability of
131.6±9.39% was observed in methanolic seed extract of A. lebbeck (50 µg/mL), and the minimum cell viability of 107.3±3.68% was observed in
acetone seed extract of C. occidentalis (50 µg/mL) comparatively.
Conclusions: The methanolic, ethanolic, and acetone extracts of seeds/leaves from A. lebbeck, C. occidentalis, and S. chirata were showed the
hepatoprotective activity. Further in vivo and clinical studies are required to confirm their therapeutic efficacy.
Keywords: Albizia lebbeck, Cassia occidentalis, Swertia chirata, Paracetamol, HepG2, [3-(4,5-dimethyl thiazol-2-yl)-5-diphenyltetrazolium bromide]
assay.
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