ASSESSMENT OF ANTINEOPLASTIC POTENTIAL OF ANNONA RETICULATA LINN. ON HUMAN CANCER CELL LINES

Authors

  • RajaRajan p Centre for Research and PG StudiesIndian Academy Degree CollegeBangalore
  • Senthil Kumar R

Abstract

ABSTRACT
Objective: The present study was undertaken to establish the antineoplastic potential of Annona reticulata on human cancer cell lines, viz., squamous
cell carcinoma 9 (SCC9), MCF-7 (human breast adenocarcinoma), A549 (lung adenocarcinoma), and HCT116 (colorectal carcinoma) cells.
Methods: The study was performed through MTT, cell cycle (G2M), and DNA fragmentation assays. Cells inhibited in MTT assay were subjected to cell
cycle analysis and DNA fragmentation, respectively.
Results: The extracts showed dose-dependent growth inhibition of SCC9, MCF-7, A549, and HCT116 cells. In contrast to HCT116 and A549 cells,
which exhibited cytotoxicity at a higher concentration with IC50 value of 271.7 and 296 µg/ml, the extract treated SCC9 and MCF-7 cells exhibited
significant cytotoxicity at a lower concentration with IC50 value of 49.47 and 52.65 µg/ml, respectively. The treated A549 and MCF-7 cells showed cell
cycle arrest up to 7.07% and 14.42%, respectively, at G2/M phase of cell cycle. DNA fragmentation was not observed in both A549 and MCF-7 cells
treated with A. reticulata extract at a concentration of 320 µg/ml.
Conclusion: Encouraging preliminary results emphasize the necessity for further research on characterization of individual compounds from this
extract and advocate it as a good source of anticancer agent for certain types of cancer.
Keywords: Annona reticulata, Anticancer activity, MTT, G2M and DNA fragmentation.

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Author Biography

RajaRajan p, Centre for Research and PG StudiesIndian Academy Degree CollegeBangalore

Head, Department of Microbiology

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Published

01-07-2016

How to Cite

p, R., and S. K. R. “ASSESSMENT OF ANTINEOPLASTIC POTENTIAL OF ANNONA RETICULATA LINN. ON HUMAN CANCER CELL LINES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 408-11, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/13028.

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