ASSESSMENT OF ANTINEOPLASTIC POTENTIAL OF ANNONA RETICULATA LINN. ON HUMAN CANCER CELL LINES
Abstract
ABSTRACT
Objective: The present study was undertaken to establish the antineoplastic potential of Annona reticulata on human cancer cell lines, viz., squamous
cell carcinoma 9 (SCC9), MCF-7 (human breast adenocarcinoma), A549 (lung adenocarcinoma), and HCT116 (colorectal carcinoma) cells.
Methods: The study was performed through MTT, cell cycle (G2M), and DNA fragmentation assays. Cells inhibited in MTT assay were subjected to cell
cycle analysis and DNA fragmentation, respectively.
Results: The extracts showed dose-dependent growth inhibition of SCC9, MCF-7, A549, and HCT116 cells. In contrast to HCT116 and A549 cells,
which exhibited cytotoxicity at a higher concentration with IC50 value of 271.7 and 296 µg/ml, the extract treated SCC9 and MCF-7 cells exhibited
significant cytotoxicity at a lower concentration with IC50 value of 49.47 and 52.65 µg/ml, respectively. The treated A549 and MCF-7 cells showed cell
cycle arrest up to 7.07% and 14.42%, respectively, at G2/M phase of cell cycle. DNA fragmentation was not observed in both A549 and MCF-7 cells
treated with A. reticulata extract at a concentration of 320 µg/ml.
Conclusion: Encouraging preliminary results emphasize the necessity for further research on characterization of individual compounds from this
extract and advocate it as a good source of anticancer agent for certain types of cancer.
Keywords: Annona reticulata, Anticancer activity, MTT, G2M and DNA fragmentation.
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REFERENCES
Heena VS, Sunil HG, Rama P. Molecular - Docking studies of potent
anticancer agent. J Comput Sci Syst Biol 2012;5(1):12-5.
International Agency for Research on Cancer, (World Health
Organization), Latest World Cancer Statistics, Press Release No. 223,
December; 2013.
Suresh K, Manoharan S, Vijayaanand MA, Sugunadevi G. Modifying
effects of Annona squamosa Linn. on glycoconjugates levels in 7, 12
dimethylbenz (A) anthracene (DMBA) induced hamster buccal pouch
carcinogenesis. J Appl Sci Res 2010;6(8):973-9.
Madhuri S, Pandey G. Some anticancer medicinal plants of foreign
origin. Curr Sci 2009;96(6):779-83.
Suresh HM, Shivakumar B, Hemalatha K, Heroor SS, Hugar DS,
Rao KR. In vitro antiproliferativeactivity of Annona reticulata roots on
human cancer cell lines. Pharmacognosy Res 2011;3(1):9-12.
Pathak K, Zaman K. An overview on medicinally important
plant - Annona reticulata Linn. Int J Pharmacogn Phytochem Res
;5(4): 299-301.
Mishra S, Shamim A, Nilesh K, Brijesh KM. Annona muricata (The
cancer killer): A review. Glob J Pharm Res 2013;2(1):1613-8.
Crouch SP, Kozlowski R, Slater KJ, Fletcher J. The use of ATP
bioluminescence as a measure of cell proliferation and cytotoxicity.
J Immunol Methods 1993;160(1):81-8.
Gonzalez RJ, Tarloff JB. Evaluation of hepatic subcellular fractions
for Alamar blue and MTT reductase activity. Toxicol In Vitro
;15(3):257-9.
Hattori N, Sakakibara T, Kajiyama N, Igarashi T, Maeda M, Murakami S.
Enhanced microbial biomass assay using mutant luciferase resistant to
benzalkonium chloride. Anal Biochem 2003;319(2):287-95.
Kangas L, Grönroos M, Nieminen AL. Bioluminescence of cellular
ATP: A new method for evaluating cytotoxic agents in vitro. Med Biol
;62(6):338-43.
Mark F. Cell viability and proliferation. Bio Files 2002;6(5):17-21.
Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival.
Modifications to the tetrazolium dye procedure giving improved
sensitivity and reliability. J Immunol Methods 1986;89(2):271-7.
Mosmann T. Rapid colorimetric assay for cellular growth and survival:
Application to proliferation and cytotoxicity assays. J Immunol
Methods 1983;65(1-2):55-63.
Thylur RP, Senthivinayagam S, Campbell EM, Rangasamy V,
Thorenoor N, Sondarva G, et al. Mixed lineage kinase 3 modulates
ß-catenin signaling in cancer cells. J Biol Chem 2011;286(43):37470-82.
Jackman J, O’Connor PM. Methods for synchronizing cells at specific
stages of the cell cycle. Curr Protoc Cell Biol 2001; Ch. 8:Unit. 8.3.
Azimian-Zavareh V, Hossein G, Janzamin E. Effect of lithium
chloride and antineoplastic drugs on survival and cell cycle of
androgen-dependent prostate cancer LNCap cells. Indian J Pharmacol
;44(6):714-21.
Takada M, Noguchi A, Sayama Y, Kurohane Kaneko Y, Ishikawa T.
Inositol 1,4,5-trisphosphate receptor-mediated initial Ca(2+) mobilization
constitutes a triggering signal for hydrogen peroxide-induced apoptosis
in INS-1 β-cells. Biol Pharm Bull 2011;34(7):954-8.
Chen YL, Lin SZ, Chang JY, Cheng YL, Tsai NM, Chen SP, et al.
In vitro and in vivo studies of a novel potential anticancer agent of
isochaihulactone on human lung cancer A549 cells. Biochem Pharmacol
;72(3):308-19.
Zhang YH, Peng HY, Xia GH, Wang MY, Han Y. Anticancer effect of
two diterpenoid compounds isolated from Annona glabra Linn Acta
Pharmacol Sin 2004;25(7):937-42.
Pardhasaradhi BV, Reddy M, Ali AM, Kumari AL, Khar A. Antitumour
activity of Annona squamosa seed extracts is through the generation
of free radicals and induction of apoptosis. Indian J Biochem Biophys
;41(4):167-72.
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