IN VIVO HEPATOPROTECTIVE ACTIVITY OF CASSIA AURICULATA POLYMER NANOSPHERES CONTAINING SILYMARIN

A. Keerthi Sagar; G. Devala Rao

doi:10.22159/ajpcr.2016.v9i5.13177

Authors

A. Keerthi Sagar Acharya Nagarjuna University, Guntur
G. Devala Rao

DOI:

https://doi.org/10.22159/ajpcr.2016.v9i5.13177

Abstract

Objective: Hepatoprotective activity of herbal drugs has an importance in the treatment of hepatic disorders, but pharmaceutically engineered
products are not evolved properly to cure the liver disorders properly based on the demand and also route, target the appropriate site of action.

Methods: In our present work, we have been developed a new formulation that possesses a unique nature and site specificity for targeting the disease
state. Here, we examined hepatoprotective activity of Cassia auriculata polymer nanospheres containing silymarin against carbon tetrachlorideinduced
hepatotoxicity in rats using at 50 mg/kg and 100 mg/kg body weight dose levels, and we have been observed that enzyme activities of serum
glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphate, total protein, albumin, globulin, total cholesterol, high-density lipoprotein (HDL), glutathione (GSH), and total bilirubin were analyzed.

Results: C. auriculata polymer nanospheres and silymarin produced significant (p<0.001) hepatoprotective effect by decreasing the activity of serum enzymes, bilirubin, total cholesterol, and increased levels of HDL, total protein, albumin, globulin, and tissue GSH.

Conclusion: From these results, it was concluded that of C. auriculata polymer nanospheres would protect the liver cells from carbon tetrachloride
from liver diseases.

Keywords: Hepatoprotective activity, Silymarin, Cassia auriculata, Nanospheres.

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Author Biography

A. Keerthi Sagar, Acharya Nagarjuna University, Guntur

Professor, Department of Pharmaceutics.

References

Brahmankar DM, Jaiswal SB. Biopharmaceutics and Pharmacokinetics-A Treatise. 1st ed. New Delhi: Vallabh Prakashan; 1995. p. 296-7.

Kingston DG. Modern natural products drug discovery and its relevance to biodiversity conservation. J Nat Prod 2011;74(2):496-511.

Jia L, Zhao Y. Current evaluation of the millennium phytomedicine â€“ ginseng (I): Etymology, pharmacognosy, phytochemistry, market and regulations. Curr Med Chem 2009;16(19):2475-84.

Gunasekaran T, Nigusse T, Dhanaraju MD. Silver nanoparticles as real topical bullets for wound healing. J Am Coll Clin Wound Spec 2012;3(4):82-96.

Sahni JK, Baboota S, Ali J. Promising role of nanopharmaceuticals in drug delivery. Pharm Times 2011;43(10):16-8.

Qureshi NA, Al-Bedah AM. Mood disorders and complementary and alternative medicine: A literature review. Neuropsychiatr Dis Treat 2013;9:639-58.

AQ4

Egert S, Rimbach G. Which sources of flavonoids: Complex diets or dietary supplements? Adv Nutr 2011;2(1):8-14.

Jain D, Raturi R, Jain V, Bansal P, Singh R. Recent technologies in pulsatile drug delivery systems. Biomatter 2011;1(1):57-65.

Lambert WJ. Considerations in developing a target product profile for parenteral pharmaceutical products. AAPS PharmSciTech 2010;11(3):1476-81.

Liong M, Lu J, Kovochich M, Xia T, Ruehm SG, Nel AE, et al. Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery. ACS Nano 2008;2(5):889-96.

Yen FL, Wu TH, Lin LT, Cham TM, Lin CC. Nanoparticles formulation of Cuscuta chinensis prevents acetaminophen-induced hepatotoxicity in rats. Food Chem Toxicol 2008;46(5):1771-7.

Boyd EM, Bereczky GM. Liver necrosis from paracetamol. Br J Pharmacol Chemother 1966;26(3):606-14.

Dahlin DC, Miwa GT, Lu AY, Nelson SD. N-acetyl-p-benzoquinone imine: A cytochrome P-450-mediated oxidation product of acetaminophen. Proc Natl Acad Sci U S A 1984;81(5):1327-31.

Mitchell JR, Jollow DJ, Potter WZ, Gillette JR, Brodie BB. Acetaminophen-induced hepatic necrosis. IV. Protective role of glutathione. J Pharmacol Exp Ther 1973;187(1):211-7.

Savides MC, Oehme FW. Acetaminophen and its toxicity. J Appl Toxicol 1983;3(2):96-111.

Gupta AK, Misra N. Hepatoprotective activity of aqueous ethanolic extract of chamomile capitula in paracetamol intoxicated albino rats. Am J Pharmacol Toxicol 2006;1(1):17-20.