PHYTOCHEMICAL PROFILE STUDIES ON THE STEROIDS OF METHANOLIC LEAF EXTRACT OF MEDICINALLY IMPORTANT PLANT Holoptelea Integrifolia (Roxb.) PLANCH USING HPTLC

Authors

  • RAVINDRA C SUTAR Sanjivani College of Pharma. Education and Research, Kopargaon, Maharashtra, India.
  • SANJAY B KASTURE Sanjivani College of Pharma. Education and Research, Kopargaon, Maharashtra, India.
  • KALAICHELVAN VK Dept. of Pharmacy,Annamalai University,Tamil Nadu, India.

Abstract

Objective: The present study was conducted to identify the steroids from the methanol extract of medicinally and economically useful leaves of
Holoptelea integrifolia (Roxb.) planch using high-performance thin layer chromatography (HPTLC) technique.
Materials and Methods: Preliminary phytochemical screening was done, and HPTLC studies were carried out. Camag HPTLC system equipped
with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at
540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp.
Results: Preliminary phytochemical screening of methanolic extract of H. integrifolia showed the presence of steroids, alkaloids, flavonoids,
proteins, and carbohydrates. HPTLC fingerprinting of steroids of methanol extract of leaf showed seven polyvalent phytoconstituents (7 peaks) and
corresponding ascending order of radio frequency (Rf) values in the range of 0.17-0.64.
Conclusions: With the above Rf values and preliminary phytochemical analysis, we have concluded the presence of steroids in methanol extract.

Keywords: Holoptelea integrifolia (Roxb.) planch leaf, Phytochemical screening, Steroids, High-performance thin layer chromatography
fingerprinting.

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Author Biographies

RAVINDRA C SUTAR, Sanjivani College of Pharma. Education and Research, Kopargaon, Maharashtra, India.

Asst. Prof. of Pharmacology

SANJAY B KASTURE, Sanjivani College of Pharma. Education and Research, Kopargaon, Maharashtra, India.

Principal

KALAICHELVAN VK, Dept. of Pharmacy,Annamalai University,Tamil Nadu, India.

Asst. Prof of Pharmacy

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Published

01-09-2014

How to Cite

C SUTAR, R., S. B. KASTURE, and K. VK. “PHYTOCHEMICAL PROFILE STUDIES ON THE STEROIDS OF METHANOLIC LEAF EXTRACT OF MEDICINALLY IMPORTANT PLANT Holoptelea Integrifolia (Roxb.) PLANCH USING HPTLC”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 4, Sept. 2014, pp. 197-00, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/1555.

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