EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF STEM AND LEAF EXTRACTS OF COSCINIUM FENESTRATUM
Abstract
Objective:In the present study, antioxidant activity and antibacterial activity leaf and stem extracts of Coscinium fenestratum was investigated.
Methods:To asses the antioxidant activity, methanolic leaf and stem extracts were used. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS. For antimicrobial activity, aqueous, acetone, ethanol and methanloic extracts of stem and leaf extracts were tested for its potent antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis.Total content of phenol and flavonoid was quantitatively estimated in leaf and stem extracts of Coscinium fenestratum.
Results &Conclusion: Total phenoilc content in the stem and leaf was found to be 8.35±0.56 and 3.35±0.67 mg GAE/g extract, While  the total flavonoid content in the stem and leaf were found to be 12.8±0.88 and 3.2±0.78 mg QE/g extract respectively. Among the different extracts, methanolic stem extract showed moderate activity against E.Coli (17±0.33),P.aeruginosa (12±0.20), B.subtilis (13±0.45).Methanol leaf extract had maximum activity against S.aureus(6.4±0.67) and lowest against B.subtilis (3.9±0.58) respectively.
Key words: Coscinium fenestratum, Antioxidant , Antimicrobial, DPPH, Medicinal plant, Phenol, Free radical.
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Balandrin MF, Kjocke AJ, Wurtele E. Natural plant chemicals: sources of industrial and mechanical materials. Science 1985; 228:1154-1160.
Bandow JE, Brotz H, Leichert LIO . Proteomic approach to understanding antibiotic action. Antimicrob Agents Chemother.2003; 47: 948-955.
Benavente-Garcia O, Castillo J, Marin FR, Ortuno A, Del Rio JA . Uses and properties of Citrus flavonoids. J Agric Food Chem 1997;45: 4505–4515.
Blois MS . Antioxidant determinations by the use of stable free radicals. Nature 1958; 26:1199- 1200
Chang C, Yang M, Wen H, Chern J . Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J.Food Drug Anal 2002; 10: 178-182.
Cook NC, Samman, S . Flavonoids- chemistry, metabolism,cardioprotective effects, and dietary sources. Nutritional Biochemistry1996; 7: 66- 76.
Hyun DH, Hernandez JO, Mattson MP, de Cabo R . The plasma membrane redox system in aging. Aging Res. Rev.2006 ; 5: 209-220.
Ito N, Fukushima S, Hagiwara A, Shibata M, Ogiso T .Carcinogenicity of butylated hydroxyanisole in F344 rats. J. Natl.Cancer Inst. 1983;70: 343-347.
Iwu MM, Duncan AR, Okunji CO . New Antimicrobials of plant origin. In Janick, J. (ed) Perspectives in New crops and New uses.ASHS Press, Alexandria, V.A. 1999; p 457-462.
Jigna Parekh and Sumitra Chanda. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research 2007;Vol. 10: 175 – 181
Kumar D and Hyde KD.Biodiversity and tissue-recurrence of endophytic fungi in Tripterygium wilfordii. Fungal Diversity 2004; 17, 69-90
McDonald S, Prenzler PD, Antolovich M, Robards K . Phenolic content and antioxidant activity of olive extracts. Food Chem. 2001; 73:73-84.
McFarland, J. Standardization of bacterial culture for the disc diffusion assay. Journal of American Medical Association 1987;49, 1176–1178.
Miliauskas G, Venskutonis PR and van Beek TA .Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem.2004; 85: 231-237
Piyanuch R, Sukhthankar M, and Baek SJ. Berberine, a natural isoquinoline alkaloid, induces NAG-1 and ATF3 expression in human colorectal cancer cells. National Institute of Health 2007; 258(2), 230–240.
Prior RL, Cao G .Antioxidant phytochemicals in fruits and vegetables. Diet and health implications. Hortic. Sci.2000; 35: 588-592.
Punitha ISR, Rajendran K, Shirwaikar A and Shirwaikar A. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin–nicotinamide induced diabetic rats. Oxford Journal 2005; 2(3), 375–381.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med.1999; 26: 1231-1237.
Remashree AB, Jayanthi A, Thushar KV, Ravindran PN and Indira Balachandran. Anatomy of Coscinium fenestratum (Gaertn.) Colebr. A critically endangered medicinal plant from Western Ghats. Phytomorphology 2005; 55, 249-258.
Rice-Evans C, Miller N, Paganga G . Antioxidant properties of phenolic compounds. Trends Plant Sci.1997; 2: 152-159.
Sas K, Robotka H, Toldi J, Vecsei L . Mitochondrial, metabolic disturbances, oxidative stress and kynurenine system, with focus on neurodegenerative disorders. J. Neurol. Sci. 2007;257: 221-239
Shirwaikar A, Shirwaikar A and Punitha ISR . Antioxidant studies on the methanol stem extract of Coscinium fenestratum. Natural Product Sciences 2007;13(1):40-45.
Tepe B, Sokmen M, Akpulat HA, Sokmen A. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem.2006; 95:200-204
Tushar KV, George S, Remashree AB and Balachandran I.Coscinium fenestratum (Gaertn.) Colebr. A review on this rare, critically endangered and highly-traded medicinal species. Journal of Plant Sciences 2008;3(2), 33-145
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