TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF ETHANOL FRACTION OF PICRIA FEL-TERRAE (LOUR.) HERBS
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i7.17982Keywords:
Phenolic, Flavonoid, Antioxidant, P fel-terrae Lour, Ethanol, HerbsAbstract
Objective: To evaluate total phenolic, flavonoid content (TFC and TPC) andantioxidant activity of ethyl acetate fraction (ETF) of Picria fel-terrae Lour. herbs.
Methods: TPC and TFC in ETF were determined by Folin–Ciocalteu and colorimetric methods, and antioxidant activity was determined by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) method.
Results: ETF was found to contain low levels of phenolic (17.71±1.26 mg gallic acid equivalent/g), total flavonoid (14.43±0.03 mg quercetin equivalents/g). Antioxidant activity from DPPH assay was measured as inhibitory concentration 25.72±0.13 μg/mL.
Conclusions: The results reveal that ETF of P. fel-terrae Lour. herbs has strong antioxidant activity. Our further study is to the isolation of compounds which responsible for antioxidant components.
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