ISOLATION ANTI-DIABETIC AND ANTIOXIDANT EVALUATION OF AQUEOUS EXTRACT OF CANSJERA RHEEDII LEAVES
Keywords:
Cansjera rheedii, antidiabetic activity, antioxidant activity, 1HNMR, Mass spectroscopyAbstract
The plant Cansjera rheedii belonging to the family Opiliaceae was selected and various studies such as phytochemical and pharmacological activities were carried out.
Plant leaves were collected and was shade dried for 15 days, until it was dry enough for extraction. It was then powdered in a grinder and extracted with solvents such as hexane, ethanol, and water.
The extracts were subjected to various preliminary phytochemical tests and the results indicated the presence of flavanoids, in the aqueous extract.
The compounds were identified by TLC and isolated by column chromatography.
The spectral studies were carried out for the aqueous extract of Cansjera rheedii  aquatic leaves and were subjected for purity testing. The purified fractions were subjected to IR (Infra red spectroscopy), 1H NMR(Nuclear Magnetic resonance spectroscopy), MASS Spectroscopy studies for the confirmation of the functional groups, number of protons and molecular weight.
The results obtained from the chemical tests, IR, 1HNMR, MASS spectroscopy gave the conclusion that Quercetin-3-O-β-rutinoside  was present in the extract.
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The anti-diabetic activity of the aqueous extract of Cansjera rheedii leaves were compared with that of standard drugs. The aqueous extract of Cansjera rheedii leaves produced significant anti diabetic activity (at doses 200mg/kg and 400mg/kg)  in dose dependent manner. The in vitro antioxidant activity was evaluated using DPPH scavenging method At 25, 50, 75, and 100 µg/ml aqueous extracts of Cansjera rheedii was found to produced better (p<0.01) DPPH scavenging activity compared to ethanolic extracts. At 250µg/ml ethanolic extract of Cansjera rheedii was found to have better (p<0.01) DPPH scavenging activity when compared to aqueous extracts of Cansjera rheedii.
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Varadarasou Mouttaya Mounnisamy,Subramanian Kavimani,Gnanapragasam Sankari,Sabarimuthu Darlin Quine and Kuppuswamy subramani.Evaluation of acute and subacute toxicity of ethanol extracts of Cansjera rheedi.Journal of Brewing and Distilling 1(1):011-014,2010.
Xi-ling zheng,Fu-wu Xing.Ethnobotanical study on medicinal plants around Mt.Yinggeling,Hainan Island,China.Journal of Ethnopharmacology 124:197-210,2009.
Leland.J.Cseke. Natural products from plants. Second edition, CRC/Taylor and Francis Publishers: 60-70, 2006.
Liqun Rao, Shiyin Guo et al., Optimization of the technology of extracting water soluble polysaccharides from Morus alba L. leaves. African Journal of Biotechnology, vol 10(59): 12714-20,2011.
Ll He, Tan Yimin, Xu Jian Ping, et al., Research Progress on polysaccharides from Ginkgo biloba. Journal of Medicinal plants and Research, vol 6(2): 171-6,2012.
Severian Dumitriu. Polysaccharides in Medicinal Applications. M.Dekker Publishers: 3-765,1996.
Shaan JJ et al., Effect of anti diabetic polysaccharide from Inula japonica on constipation in normal and two models of experimental constipated mice. Phytother Res, 24(11): 1734-8, 2010.
OECD Guidelines for testing Chemicals, Guidelines 425, acute oral toxicity: Acute toxic class method.1996.Paris.
Gholap S and Kar A. Effects of Inula racemosa root and Gymnema sylvestre leaf extracts in the regulation of corticosteroid induced diabetes mellitus: involvement of thyroid hormones. Pharmazie, vol 58(6): 413-5, 2003.
Gholap S et al., Inula racemosa, Boerhaavia diffusa and Ocimum sanctum (Holy Basil) exhibited anti-peroxidative, hypoglycemic and cortisol lowering activities- Green Medinfo Summary. Pharmazie, 59(11):876-8, 2004.
Gaafar A M, Serag Ei Din M F, Boudy E A et al. Extraction conditions of Inulin from Jerusalem Artichoke tubers and its effects on Blood glucose and Lipid Profile in diabetic rats. Journal of American Science, vol 6(5): 36-43, 2010.
Nooman A. Khalaf, Ashok K. shakya, Atif al-othman, Zaha el-agbar, Husni farah, Antioxidant Activity of Some Common Plants, Turk J Biol 32,51-55,2008.
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