INHIBITION OF PYOCYANIN PRODUCTION IN PSEUDOMONAS AERUGINOSA BY NATURAL ANTIMICROBIAL COMPOUNDS FROM HERBAL EXTRACTS

Preeti Jain; Suman Kumari; Ashish Malik

doi:10.22159/ajpcr.2017.v10i6.18056

Authors

Preeti Jain 1Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, India
Suman Kumari Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, India
Ashish Malik Department of Botany, Maharshi Dayanand University, Rohtak, Haryana, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i6.18056

Keywords:

Pseudomonas aeruginosa, Pyocyanin, Antibacterial activity, Chloroform extraction assay, Herbal extract

Abstract

Objective: P. aeruginosa produces a range of metabolites including pyocyanin that enhance its ability to resist antibiotics and becomes capable of surviving adverse conditions.

Method: In this report, eight plants (extracted with five solvents) were screened for antibacterial activity against P. aeruginosa by microbroth dilution method. Afterward tested for inhibition of pyocyanin in presence and absence of plant extracts.

Result: Among these D. muricata and S. quitoenes exhibited good antibacterial potential. Inhibition of pyocyanin is identified as a potential anti-virulence strategy. Therefore, acetone extract of six plants exhibited MIC â‰¤3.125mg/ml and methanol extract of three plants exhibited MIC â‰¤6.25mg/ml were used to check pyocyanin inhibition in P. aeruginosa. In acetone extracts, significant pyocyanin inhibition was found in I. pestigirdis and C. colocynthis. In methanol extracts, C. colocynthis and D. muricata showed considerable pyocyanin inhibition.

Conclusion: Overall result indicates that the best antimicrobial compound (growth inhibitor) may not be best inhibitor of pyocyanin biosynthesis or vice-versa. Moreover, I. pestigirdis, C. colocynthis and D. muricata seems to contain compounds which inhibit the growth of bacteria as well as the biosynthesis of pyocyanin.Â

Downloads

Download data is not yet available.

References

Marshall BC, Carroll KC. Interaction between Pseudomonas aeruginosa and host defenses in cystic fibrosis. Semin Respir Infect 1991;6(1):11-8.

Gupta MP, Solis PN, CalderÃ³n AI, Guinneau-Sinclair F, Correa M, Galdames C, et al. Medical ethnobotany of the tribes of Bocas del Toro. Panama. J Ethnopharmacol 2005;96:389-401.

Cox CD. Role of pyocyanin in the acquisition of iron from transferrin. Infect Immun 1986;52(1):263-70.

Ran H, Hassett DJ, Lau GW. Human targets of Pseudomonas aeruginosa pyocyanin. Proc Natl Acad Sci U S A 2003;100(24):14315-20.

Diggle SP, Winzer K, Chhabra SR, Worrall KE, CÃ¡mara M, Williams P. The Pseudomonas aeruginosa quinolone signal molecule overcomes the cell density-dependency of the quorum sensing hierarchy, regulates rhl-dependent genes at the onset of stationary phase and can be produced in the absence of LasR. Mol Microbiol 2003;50(1):29-43.

Irda F, Evelyne N, Komar RW. In vitro antioxidant activities, total flavonoid, phenolic and carotenoid content from various extracts of four species Asteraceae herb. Int J Pharm Pharm Sci 2017;7:192-7.

Katiyar D, Singh V, Ali M. Isolation, characterization and prediction of biological activity of two new fatty esters and a phenol from the heartwood of Pterocarpus marsupium roxb. Int J Pharm Pharm Sci 2017;9:117-22.

Kopec DM, Gilbert J, Pessarakli M. Penoxsulam as a Potential Post-Emergence for Khakiweed (Alternanthera pungens). 2004. Available from: http://www.cals.arizona.edu/pubs/crops/az1359.

Onocha PA, Ajaiyeoban EO, Dosumu OO, Ekundayo O. Phytochemical screening and biological activities of Gomphrena celosioides (C. Mart.) extracts. Nigerian Soc Exp Biol J 2005;5:59-65.

Gancel AL, Alter P, Dhuique-Mayer C, Ruales J, Vaillant F. Identifying carotenoids and phenolic compounds in Naranjilla (Solanum quitoense Lam. var. Puyo hybrid), an andean fruit. J Agric Food Chem 2008;56:11890-9.

Rai MK. A review on some antidiabetic plants of India. Anc Sci Life 1995;14(3):168-80.

Putt ED. Early history of sunflower. Sunflower Technol Prod 1997;35:1-19.

Prajapati MS, Patel JB, Modi K, Shah MB. Leucas aspera: A review. Pharmacogn Rev 2010;4(7):85-7.

Khond M, Bhosale JD, Arif T, Mandal TK, Padhi MM, Dabur R. Screening of some selected medicinal plants extracts for in-vitro antimicrobial activity. Middle East J Sci Res 2009;4:271-8.

Essar DW, Eberly L, Crawford IP. Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC. J Bacteriol 1990;172(2):867-83.