RELEVANCE AND PERSPECTIVES OF EXPERIMENTAL WOUND MODELS IN WOUND HEALING RESEARCH

Santram Lodhi; Gautam P Vadnere

doi:10.22159/ajpcr.2017.v10i7.18276

Authors

Santram Lodhi Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon - 425 107, Maharashtra, India.
Gautam P Vadnere Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Jalgaon - 425 107, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i7.18276

Keywords:

Wound healing, Animal models, Cell culture, Burn, Ischemic wound, Diabetic

Abstract

The wound healing process consists of four highly integrated and overlapping phases: Hemostasis, inflammation, proliferation, and tissue remodeling. These phases and their biophysiological functions must occur in the proper sequence, at a specific time and continue for a specific duration at an optimal intensity. There are many factors that can affect wound healing which interferes with one or more phases in this process, thus causing improper or impaired tissue repair. This review was aimed to collect data and made a critical analysis. This will provide concise information regarding different models and parameters used for wound healing study. The data related to different wound models are collected using popular search engines as well as relevant science search engines and database including Google Scholar, Science Direct, and PubMed. A new drug substance can be evaluated for wound healing activity using different in vitro models such as cell culture, chick chorioallantoic membrane model, tube formation on metrigel and capillary growth model. The in vivo wound models such as incision, excision, dead space, burn wound, ischemic wound, and diabetic wound models are frequently used. Each model has specific importance. The limitations and advantages of each are described in this review. Although animal wound repair is an imperfect reflection of human wound healing and its clinical challenges, these models can be fundamental tools for the development of new approaches to rational wound therapy.

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References

Mast BA. The Skin. In: Cohen IK, Diegelmann RF, Linblade WJ, editors. Wound Healing Biochemical and Chemical Aspects. Philadelphia, PA: WB Saunders Company; 1992. p. 344-55.

Yardley PA. A Brief History of Wound Healing. Oxford: Clinical Communication Inc.; 1998.

Shukla A, Patnaik GK. Wound healing: Molecular mechanism. Proc Nat Acad Sci India 1998;LXVIII:1-13.

Charde MS, Fulzele SV, Satturwar PM, Dork AK. Wound healing activity of Durva ghrita. Indian J Pharm Sci 2003;65(5):482-5.

Mukherjee PK. Quality Control of Herbal Drugs. 1st ed. New Delhi: Business Horizons; 2002. p. 546-8.

Gottrup F. Experimental wound healing research: The use of model. EWMA J 2001;1(2):5-8.

Tortora GJ, Grabowski SR. Principle of Anatomy and Physiology. 7th ed. New York: Harper Collins College Publishers; 1993. p. 135-7.

Clark RA, editor. Wound repair: Overview and general considerations. In: The Molecular and Cellular Biology of Wound Repair. New York: Plenum; 1996. p. 3-50.

DiPietro LA, Burns AL. Wound Healing: Methods and Protocols. Totowa, NJ, USA: Humana Press, Inc.; 2003.

Jeffery MD. Experimental animal models. Wounds 2001;13(1):9-23.

Hemalata S, Subramanian N, Ravichandran V, Chinnaswamy K. Wound healing activity of Indigofera ennaphylla Linn. Indian J. Pharm Sci 2001;63(4):331-3.

Taranalli AD, Tipare SV, Kumar S, Torgal SS. Wound healing activity of Oxalis corniculata whole plant extract in rats. Indian J Pharm Sci 2004;66(4):444-6.

Shirwaikar A, Shenoy R, Udupa AL, Shetty S. Wound healing property of ethanolic extract of leaves of Hyptis suaveolens with supportive role of antioxidant enzymes. Indian J Exp Biol 2003;41:238-41.

Patil MB, Jalalpure JS, Ashraf A. Preliminary phytochemical investigation and wound healing activity of the leaves of Argemone maxicana Linn. (Papaveraceae). Indian Drugs 2001;36(6):288-93.

Sultana Y, Aquil M, Ali A. Burn and its management. Humdard Med 2004;54(1):66-71.

Knabl JS, Bayer GS, Bauer WA, Schwendenwein I, Dado PF, Kucher C, et al. Controlled partial skin thickness burn: An animal model for studies of burn wound progression. Burn 1999;25:229-35.

Walker HL, Mason AD. A standard animal burn. J Trauma 1968;8:1049 51.

Yalcin O, Soybir G, Koksoy F, Kose H, Ozturk R, Cokneseli B. Effect of granulocyte colony stimulating factor on bacterial translocation due to burn wound sepsis. Surg Today 1997;27:154-8.

Nakae H, Inaba H. Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model. J Trauma 2000;49(3):511 4.

Bairy KL, Abhinav R, Satyam SM. Evaluation of burn wound healing activity of topical regular insulin in non-diabetic and streptozocin-induced diabetic rats. Int J Pharm Pharm Sci 2014;6(8):127-30.

Chin G, Gowda S, Schultz G. Evaluation of platelet-derived growth factor in a rat model of ischemic skin wound healing. Wounds 2002;14(5):199-203.

Blalock EM, Chen KC, Vanaman TC, Landfield PW, Slevin JT. Epilepsy-induced decrease of L-type Ca2+ channel activity and coordinate regulation of subunit mRNA in single neurons of rat hippocampal â€˜zipperâ€™ slices. Epilepsy Res 2001;43:211-6.

Ghalbzouri AE, Hensbergen P, Gibbs S, Kempenaar J, Schors R, Ponec M. Fibroblasts facilitate re-epithelization in wounded human skin equivalents. Lab Invest 2004;84:102-12.

Maggelakis SA. Modeling the role of angiogenesis in epidermal wound healing. Discrete Continuous Dyn Syst Seri B 2004;4(1):267-73.

Imamura M. Extracellular matrix components and angiogenesis. Nippon Yakurigaku Zasshi 1996;107(3):153-60.

Goldenberg NA. Elucidating angiogenesis: The role of basement membrane proteolysis and endothelial cell motility and proliferation. MJM 1995;1:127-37.

Maheshwari RK, Srikantan V, Bharitiya D, Kleinman HK, Grant DS. Differential effect of interferon gamma and alpha on in-vitro model of angiogenesis. J Cell Physiol 1991;146(1):164-9.

Marales DE, McGowan KA, Grant DS, Maheshwari S, Bhartiya D, Cid MC, et al. Estrogen promotes angiogenesis activity in human vein endothelial cell in-vitro and in a murine model. Circulation 1995;91:755-63.

Kilarski WW, Samolov B, Petersson L, Kvanta A, Gerwins P. Biomechanical regulation of blood vessel growth during tissue vascularization. Nat Med 2009;15(6):657-64.

Hirapara HN, Ghori VM, Anovadiya AP, Tripathi CB. Effect of amlodipine and enalapril on wound healing in diabetic Wistar albino rats. Int J Pharm Pharm Sci 2016;8(7):257-61.

Shukla R, Rai G, Jain AP. Preclinical evaluation of Alpinia galanga rhizomes for wound healing activity with reducing oxidative status. Asian J Pharm Pharmacol 2015;1(2):65-9.

Saha K, Mukherjee PK, Das J, Pal M, Saha BP. Wound healing activity of Leucas lavandulaefolia Rees. J Ethanopharmacol 1997;56:139-44.

Singh S, More PK, Shukla R. Ameliorative effect of flavonoids rich extract from Plantago lanceolata for cutaneous wound healing with antioxidant mechanism. Adv Pharm J 2016;1(1):21-5.

MCmanus JF, Mowry RW. Staining methods, histologic and histochemical. New York, Evanston, London: Harper and Row; 1965.

Udupa SL. Inhibition of lysyl oxidase by isoniazid and its effect on wound healing. Indian J Exp Biol 1995;33:278-80.

Burton K. A study of the condition and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956;62:315-21.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.

Woessner JF. The determination of hydroxyproline in tissue and protein samples containing small portion of this imino acid. Arch Biochem Biophys 1961;193:440-7.

Chithra P, Sajithlal GB, Chandrakasan G. Influence of Aloe vera on collagen turnover in healing of dermal wounds in rats. Indian J Exp Biol 1998;36:896-901.

Synder SH, Hendley ED. In: Tabor H, Tabor CW, editors. Methods in Enzymology. Vol. 17B. New York: Academic Press; 1971. p. 741.

Trackman PC, Kagan HM. Nonpeptidyl amine inhibitors are substrates of lysyl oxidase. J Biol Chem 1979;254(16):7831-6.