ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS

Anupriya Asaithambi; Manjula Gunasekaran; Manivelan S; Palaniappan Nainar

doi:10.22159/ajpcr.2017.v10i8.18343

Authors

Anupriya Asaithambi Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
Manjula Gunasekaran Department of Microbiology, Madurai Medical College, Madurai, Tamil Nadu, India
Manivelan S Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
Palaniappan Nainar Department of Microbiology, Sree Mookambika Institute of Medical Sciences, Kulasekharam, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i8.18343

Keywords:

Antinuclear antibodies, Bad obstetric history, Indirect immunofluorescence

Abstract

Objective:This study aims to determine the prevalence of antinuclear antibodies in pregnant women with bad obstetric history (BOH) against healthy multigravid women in and around Tirunelveli district

Methods: This is a case-control study comprising 60 antenatal women with BOH against 60 multiparous pregnant women with no history of abortions. Antinuclear antibodies (ANA) were detected using indirect immunofluorescence with Hep-2 cell substrate, and enzyme-linked immunosorbent assay (ELISA).

Results: Among BOH cases 19 (82.6%), 18 (78.26%) were positive by ELISA and indirect fluorescence antibody test (IFAT) method, respectively. Among controls, 4(17.39%) and 5(21.73%) individuals were positive by ELISA and IFAT methods, respectively. Of the 18 positives, homogenous pattern was most common followed by anticentromere pattern, fine speckled and coarse speckled pattern

Conclusion: IFAT is considered to be gold standard in the diagnosis of autoimmune disorders, but ELISA appears to be a suitable simple alternative for testing rheumatological disorders.

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Author Biographies

Anupriya Asaithambi, Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.

Asst Professor in Microbiology,

Chennai Medical College Hospital & research Centre

Manjula Gunasekaran, Department of Microbiology, Madurai Medical College, Madurai, Tamil Nadu, India

Asst Prof in Microbiology,

Madurai Medical College

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