ANTIBIOTIC RESISTANCE PROFILING OF DAIRY WASTEWATER DEGRADING NATIVE EFFICIENT MICROBIAL ISOLATES
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i7.18444Keywords:
Dairy wastewater, Biological treatment, Antibiotic resistance, Microbial isolatesAbstract
Objective: The objective of this study is to make sure biotreatment process used for treatment of dairy wastewater (DWW) is safe for human and its surrounding environment; microbes were evaluated for their antibiotic resistance profile against commonly prescribed antibiotics.
Methods: Microbes were isolated using spread plating and streaking method and used to treat DWW. Reduction in organic load in DWW was determined by comparing physicochemical parameters (PCP) of DWW before and after treatment process. After selection of efficient microbial isolates, they were evaluated for their antibiotic resistance profile using antibiotic disc diffusion method.
Results: In this work, 53 microbes were isolated from DWW, and these microbial isolates were screened for DWW degradation capacity by analyzing PCP. Four microbial isolates E3, E5, E11 (bacterial isolates) and F5 (fungal isolate) showed highest reduction in chemical oxygen demand (COD), biological oxygen demand (BOD), and dissolved oxygen (DO) were selected for profound degradation of DWW under optimized conditions. Efficient four microbial isolates individually performed better under anaerobic conditions by showing maximum reduction 84%, 75%, and 77% in COD, BOD, and DO, respectively. After 72 hrs of antibiotic susceptibility testing, E3 strain had shown 100%, E5 90%, E11 70%, and F5 80% susceptibility to antibiotics.
Conclusion: The present study concluded that four microbial isolates had the potential of reducing the organic load of DWW along with lessor or negligible adverse effect on human or its surrounding environment and they appear to be most promising strains for treatment of DWW.Â
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Dhall P, Siddiqi TO, Ahmad A, Kumar R, Kumar A. Restructuring BOD: COD ratio of dairy milk industrial wastewaters in BOD analysis by formulating a specific microbial seed. Sci World J 2012;2012:1-7.
Pathak U, Das P, Banerjee P, Datta S. Treatment of wastewater from a dairy industry using rice husk as adsorbent: Treatment efficiency, isotherm, thermodynamics, and kinetics Modelling. J Thermodyn 2016;2016:1-8.
Porwal HJ, Mane VA, Velhal GS. Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge. World Resour Ind 2015;9:1-15.
Sirisha E, Rajasekar N, Narasu LM. Isolation and optimization of lipase producing bacteria from oil contaminated soils. Adv Biol Res 2010;4(5):249-52.
Mahalingam UP, Sampath N. Optimization of growth condition for diesel oil degrading bacterial strains. Adv Appl Sci Res 2014;5(6):91-6.
Jayanthi P, Prabhu S. Optimization studies on degradation of endosulfan by indigenous soil bacterial isolates. Int J Sci Res 2013;5(4):2014-8.
Prasad MP, Bhakat P, Chatterjee S. Optimization of textile dye degradation by bacterial species isolated from natural sources. J Ecol Environ Sci 2013;4(1):1-6.
Svahn KS, Göransson U, El-Seedi H, Bohlin L, Larsson DJ, Olsen B, et al. Antimicrobial activity of filamentous fungi isolated from highly antibiotic-contaminated river sediment. Inf Ecol Epidemiol 2012;2:1-6. 9. Sharma A, Menghani E. Microorganisms as chemical factories for isolation of meta-bolomes from mesophilic soil. Int J Pharm Pharm Sci 2017;9(4):121-8.
Zana I, Agim S, Ardiana M, Fehim H. The possibility of development of antibiotic resistance from massive and uncontrolled use of sulfonamides in milk. Int J Pharm Pharm Sci 2017;9(4):207-11.
Sonune NA, Mungal NA, Kamble SP. Study of physico-chemical characteristics of domestic wastewater in Vishnupuri, Nanded, India. Int J Curr Microbiol Appl Sci 2015;4(1):533-6.
Sawyer C, McCarty P, Parkin G. Chemistry for Environmental Engineering and Science. 5th ed. New York: McGraw-Hill; 2003.
Prasad MP, Manjunath K. Comparative study on biodegradation of lipid-rich wastewater using lipase producing bacterial species. IJBT Niscair 2010;10:121-4.
Davis KE, Joseph SJ, Janssen PH. Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl Environ Microbiol 2005;71(2):826-34.
Bhardwaj A, Chaman S. Antibiotic susceptibility of biodegrading bacterial isolates from dairy effluent. Int J Bioassays 2013;2(7):956-60.
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