HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF OXALIS DEBILIS KUNTH AGAINST CCL4 - INDUCED LIVER DAMAGE

Rojini Athokpam; Meenakshi Bawari; Manabendra Dutta Choudhury

doi:10.22159/ajpcr.2017.v10i9.19100

Authors

Rojini Athokpam Department of Life Science and Bioinformatics, Assam University, Silchar-788011, Assam, India. http://orcid.org/0000-0001-7222-7755
Meenakshi Bawari Department of Life Science and Bioinformatics, Assam University, Silchar-788011, Assam, India.
Manabendra Dutta Choudhury Department of Life Science and Bioinformatics, Assam University, Silchar-788011, Assam, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19100

Keywords:

Oxalis debilis, Hepatoprotective, Carbon tetrachloride, Liver, Transaminases

Abstract

Â

Â Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.

Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin.

Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins.

Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage.

Downloads

Download data is not yet available.

Author Biography

Rojini Athokpam, Department of Life Science and Bioinformatics, Assam University, Silchar-788011, Assam, India.

Research Scholar

Dept. of Life Science and Bioinformatics

References

Mushtaq A, Masoodi MH, Wali AF, Ganai BA. Multiple treatment of Eremurus himalaicus extracts ameliorates carbon tetrachloride induced liver injury in rats. Int J Pharm Pharm Sci 2016;8(9):24-7.

Sharma J, Gairola S, Gaur RD, Painuli RM. The treatment of jaundice with medicinal plants in indigenous communities of the Sub-Himalayan region of Uttarakhand, India. J Ethnopharmacol 2012;143(1):262-91.

Anusha M, Venkateswarlu M, Prabhakaran V, Taj SS, Kumari BP, Ranganayakulu D. Hepatoprotective activity of aqueous extract of Portulaca oleracea in combination with lycopene in rats. Indian J Pharmacol 2011;43(5):563-7.

Joshi BC, Prakash A, Kalia AN. Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats. Toxicol Rep 2015;2:1101-10.

Luo S, Zhang D, Renner SS. Oxalis debilis in China: Distribution of flower morphs, sterile pollen and polyploidy. Ann Bot 2006;98(2):459-64.

Dutta U. Wild vegetables collected by the local communities from the chirang reserve forest of BTAD. Assam Int J Sci Adv Technol 2012;2(4):116-25.

Adhikari B, Babu M, Saklani P, Rawat G. Medicinal plants diversity and their conservative status in wildlife institute of India (WII) campus, Dehradun. Ethnobot Leafl 2010;14:46-83.

Begum SS, Gogoi R. Herbal recipe prepared during Bohag or Rongali Bihu in Assam. Indian J Tradit Knowl 2007;6:417-22.

Saikia P, Khan ML. Diversity of medicinal plants and their uses in home gardens of upper Assam, Northeast India. Asian J Pharm Biol Res 2011;1(3):296-309.

Yang CC, Fang JY, Hong TL, Wang TC, Zhou YE, Lin TC. Potential antioxidant properties and hepatoprotective effects of an aqueous extract formula derived from three Chinese medicinal herbs against CCl4-induced liver injury in rats. Int Immunopharmacol 2013;15(1):106-13.

Evans WC. Trease and Evans Pharmacognosy. 4th ed. Singapore: Harcourt Brace and Company, Asia Pvt Ltd.; 1997.

Ramakhrishnan S, Rajan R. Textbook of Medical Biochemistry. 2nd ed. New Delhi, India: Orient Longman; 1994.

Kokate CK. Practical Pharmacognosy. 4th ed. New Delhi, India: Vallabh Prakashan Publication; 1999.

Fisher DB. Protein staining of ribboned epon sections for light microscopy. Histochemie 1968;16(1):92-6.

Ruthman AC. Methods in Cell Research. Ithaca, New York: Cornell University Press; 1970.

Gahan PB. Plant Histochemistry and Cytochemistry: An Introduction. U.S.A: Academic Press; 1984.

Finar IL. Stereo Chemistry and the Chemistry of Natural Products. Vol. 2. Singapore: Longman; 1986.

Mace ME. Histochemical localization of phenols in healthy and diseased banana roots. Physiol Plant 1963;16(4):915-25.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983;54(4):275-87.

Vuda M, Dâ€™Souza R, Upadhya S, Kumar V, Rao N, Kumar V, et al. Hepatoprotective and antioxidant activity of aqueous extract of Hybanthus enneaspermus against CCl4-induced liver injury in rats. Exp Toxicol Pathol 2012;64:855-9.

Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28(1):56-63.

Kind PR, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol 1954;7(4):322-6.

Jendrassik L, Grof P. Simplified photometric methods to determine the blood bilirubin. Biochem Z 1938;297:81-9.

Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949;177(2):751-66.

Rajalingam D, Varadharajan R, Palani S. Evaluation of hepatoprotective and antioxidant effect of Combretum albidum G. Don

against CCl4 induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2016;8(9):218-23.

Sharma S, Mehta BK. Comparative study of alcoholic and aqueous extracts of Syzygium cumini on carbon tetrachloride-induced hepatotoxicity in Wistar rats. Asian J Pharm Clin Res 2016;9:3-6.

Gao Q, Zhao X, Yin L, Zhang Y, Wang B, Wu X, et al. The essential oil of Artemisia capillaris protects against CCl4 induced liver injury in vivo. Braz J Pharmacogn 2016;26(3):369-74.

Kumarappan C, Vijayakumar M, Thilagam E, Balamurugan M, Thiagarajan M, Senthil S, et al. Protective and curative effects of polyphenolic extracts from Ichnocarpus frutescense leaves on experimental hepatotoxicity by carbon tretrachloride and tamoxifen. Ann Hepatol 2011;10(1):63-72.

Aneja S, Vats M, Aggarwal S, Sardana S. Phytochemistry and hepatoprotective activity of aqueous extract of Amaranthus tricolor Linn. Roots. J Ayurveda Integr Med 2013;4(4):211-5.