IN VITRO AND IN SILICO EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY OF QUERCETIN CONTAINED IN SONCHUS ARVENSIS LEAF EXTRACT

Rini Hendriani; Nursamsiar Nursamsiar; Ami Tjitraresmi

doi:10.22159/ajpcr.2017.v10s2.19486

Authors

Rini Hendriani Depatrment of Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
Nursamsiar Nursamsiar Department of Sekolah Tinggi Ilmu Farmasi Makassar, Indonesia.
Ami Tjitraresmi Depatrment of Faculty of Pharmacy, Universitas Padjadjaran, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s2.19486

Keywords:

Quercetin, Sonchusarvensis, Inhibitor, Xanthine oxidase, Hyperuricemia

Abstract

Objective: The aim of the present study was to examine the inhibiting effects of quercetin contained in Sonchusarvensis leaf extract on the activity of xanthine oxidase, an essential enzyme for uric acid synthesis.

Methods: Activity test was conducted in vitro by measuring the activity of xanthine oxidase using UV spectrophotometry and in silico by determining the interaction of quercetin and allopurinol (as comparation drug) with xanthine oxidase enzyme in terms of hydrogen bonds and binding free energy. Docking simulations were performed by Autodock4.2 package.

Results: The active fraction, using the solvent n-hexane, ethyl acetate and water, tested the inhibitory activity of the xanthine oxidase enzyme in vitro obtained respectively IC₅₀ of 263.19, 16.20 and 141.80 Î¼g/ml. Isolates with highest activity identified as quercetin. The xanthine oxidase enzyme inhibitory activity insilico by molecular docking showed quercetin has free energy binding Ë—7.71 kcal/mol, more negative than that of allopurinol Ë—5.63 kcal/mol.

Conclusion: This shows the affinity of quercetin stronger than that of allopurinol; so that it can be predicted that quercetin was more potential to inhibit xanthine oxidase enzyme activity. Thus the extract of the S. arvensis leaves containing the active compound quercetin was a potential use as antihyperuricemia.

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