ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD

Soul Shekhar Sp; Manjulatha K; Satyanarayan Nd

doi:10.22159/ajpcr.2017.v10i9.19548

Authors

Soul Shekhar Sp Department of Pharmaceutical Chemistry, Kuvempu University, Postgraduate Centre, Kadur, Karnataka, India.
Manjulatha K Department of Biochemistry, School of Life Science, University of Hyderabad, Gachibowli, Hyderabad, Telangana, India.
Satyanarayan Nd Department of Pharmaceutical Chemistry, Kuvempu University, Postgraduate Centre, Kadur, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19548

Keywords:

MTT assay, CT-DNA, Ethidium bromide, Agarose gel electrophoresis, Leukemia, MCF-7 cell line, K562 cell line, HePG2 cell line

Abstract

Objective: Cancer has become the major disease by uncontrolled cell growth effecting large population on the globe. This study, deals with antiproliferative activity of different solvent extracts, viz., n-hexane (S1), dichloromethane (DCM) (S2), and methanol (S3) of Acacia farnesiana pod on four cancer cell lines, viz., chronic myelogenous leukemia (K562), breast cancer (MCF-7), hepatocellular carcinoma (HePG2), colorectal adenocarcinoma (Colo 205), and DNA cleavage activity of the extracts on CT-DNA.

Methods: The antiproliferative study was performed by MTT assay and DNA cleavage studies of the solvent extracts (S1, S2, and S3) and its fractions (S4 and S5) was carried out by agarose gel electrophoresis method.

Results: The antiproliferative activity results revealed that n-hexane extract (S1) has showed activity against MCF-7 (21.70 %) cell line and methanol extract (S3) against K562 (24.5%) and HePG2 (23.3%) cell lines. The DNA cleavage could be seen at every concentration tested by n-hexane (S1), DCM (S2), and methanol (S3) extracts and significant cleavage was observed at concentrations of 25, 50, and 100 Î¼g by fractions (S4) and (S5) of methanol extract.

Conclusion: The results indicated that the extract of A. farnesiana pod (n-hexane and methanol) has antiproliferative properties and the DNA cleavage studies performed on CT-DNA was found that the extracts and its fractions showed significant activity at the concentrations tested.

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