ANTIMICROBIAL ACTIVITY OF ANISOCHILUS CARNOSUS (L.F.) WALL AGAINST THE HUMAN GASTRIC PATHOGEN HELICOBACTER PYLORI

Vignesh Shetty; Richard Lobo; Nimmy Kumar; Ramachandra Lingadakai; Ganesh C Pai; Girish Balaraju; Mamatha Ballal

doi:10.22159/ajpcr.2017.v10i10.20304

Authors

Vignesh Shetty Department of Microbiology, Enteric Diseases Division, Central Research Lab, Kasturba Medical College, Manipal University, Manipal - 576 104, Karnataka, India
Richard Lobo Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Nimmy Kumar Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Ramachandra Lingadakai Department of Surgery, Kasturba Medical College, Manipal University, Manipal - 576 104, Karnataka, India.
Ganesh C Pai Department of Gastroenterology & Hepatology, Kasturba Medical College, Manipal University, Manipal - 576 104, Karnataka, India.
Girish Balaraju
Mamatha Ballal Department of Microbiology, Enteric Diseases Division, Central Research Lab, Kasturba Medical College, Manipal University, Manipal - 576 104, Karnataka, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i10.20304

Keywords:

Anisochilus carnosus, Helicobacter pylori, Minimum inhibitory concentration, Antimicrobial activity

Abstract

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Â Objective: The prevalence of Helicobacter pylori in India is high, and majority leads to severe gastrointestinal infections. Existing treatment regimens for H. pylori infections have increased failure rates and adverse side effects that desire the search for an effective substitute therapy. Anisochilus carnosus (L.f.) wall (Lamiaceae), a herb which grows once in a year at high elevation is used widely in traditional treatment for the complaints of gastric ulcer and skin diseases. The present study was performed to assess the antibacterial activity of A. carnosus (L.f.) wall, against clinical isolates of H. pylori in vitro.

Methods: A. carnosus leaves were collected-dried and extracted with water and ethanol by cold maceration with ethanol by soxhlet method. The minimum inhibitory concentration (MIC) of extracts was made and tested against 32 clinical and 1 reference strains of H. pylori.

Results: A. carnosus (L.f.) wall inhibited the growth of most of the clinical H. pylori strains. The MIC of A. carnosus (L.f.) wall extracted by cold maceration (aqueous and ethanol) and Soxhlet apparatus (ethanol) ranged from 500 to 62.5 Î¼g/ml, and the majority of the clinical H. pylori strains were inhibited at the MIC of 500 Î¼g/ml of aqueous, ethanol, and Soxhlet ethanol extraction were 63.63%, 43.75%, and 71.87%, respectively.

Conclusion: A. carnosus (L.f.) wall is an efficient inhibitor of H. pylori growth in vitro. A. carnosus (L.f.) wall revealed enormous therapeutic potential to H. pylori infection as it was extremely active in the suppression of H. pylori. Hence, it can be taken as a potential agent against several H. pylori linked gastric pathogenic progressions.

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