ANTICANCER ACTIVITY OF GARCINIA MORELLA CHLOROFORM FRACTION AND ITS ACTIVE COMPOUND GARCINOL ON NEUROBLASTOMA

Bhaswati Choudhury; Raghuram Kandimalla; Rupjyoti Bharali; Jibon Kotoky

doi:10.22159/ajpcr.2017.v10i12.21066

Authors

Bhaswati Choudhury Drug Discovery Laboratory, Life sciences division, Institute of Advanced studies in Science and technology, Drug Discovery Lab., Paschim Boragaon, Guwahati, Assam, India.
Raghuram Kandimalla Drug Discovery Laboratory, Life sciences division, Institute of Advanced studies in Science and technology, Drug Discovery Lab., Paschim Boragaon, Guwahati, Assam, India.
Rupjyoti Bharali Department of Biotechnology, Gauhati University, Guwahati, Assam, India.
Jibon Kotoky Drug Discovery Laboratory, Life sciences division, Institute of Advanced studies in Science and technology, Drug Discovery Lab., Paschim Boragaon, Guwahati, Assam, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.21066

Keywords:

Garcinia morella, Neuroblastoma, Garcinol, Anticancer activity

Abstract

Objective: The aim of the present study is to evaluate the anticancer activity of G. morella fruit chloroform fraction and its isolated bioactive molecule garcinol on neuroblastoma cell line (SH-SY5Y).

Method: Methanol extraction was performed for the G. morella fruits through cold maceration and further fractionated with chloroform. The presence of Garcinol was confirmed by measuring the melting point. Further, the bioactive chloroform fraction and pure Garcinol was tested for anticancer activity against SH-SY5Y cells through MTT assay.

Result: The present study reveals the anticancer ability of bioactive chloroform fraction of G. morella fruit and its active molecule garcinol.

Conclusion: G. morella fruit and its bioactive compound Garcinol have significant activity against neuroblastoma. This study opens an avenue to further elucidate the mechanism of action and development of alternative treatment of this dreaded disease.

Â

Downloads

Download data is not yet available.

References

Pathare YS, Wagh VD. Herbal medicines and nutritional supplements used in the treatment of Glaucoma: A review. Res J Pharm Biol Chem Sci 2012;3(1):331-9.

Newman DJ, Cragg GM. Natural Products as Sources of New Drugs from 1981 to 2014. J Nat Prod 2016;79(3):629-61.

Rao KV, Rao PL. Antibiotic principles of Garcinia morella: IX. Antimicrobial activity of alpha & beta-guttiferins & their derivatives. Indian J Exp Biol 1967;5(2):101-5.

Santhanam K, Narasimha Rao PL. Antibiotic principles of Garcinia morella: 13 Antimicrobial activity and toxicity of alpha 1 and Y guttiferins and their derivatives. Indian J Exp Biol 1969;7(1):34-6.

Abah AA, Agbelusi GA, Odukoya OA, Ayanbadejo PO, Adefule-Ositelu AO, Adebiyi KE. Garcinia Kola extracts as antifungal therapy for oral Candida infections a comparative study. J Dent Med Sci 2014;13(11):20-4.

Tan WN, Khairuddean M, Wong KC, Tong WY, Ibrahim D. Antioxidant compounds from the stem bark of Garcinia atroviridis. J Asian Nat Prod Res 2016;18(8):804-11.

Chen LG, Yang LL, Wang CC. Anti-inflammatory activity of mangostins from Garcinia mangostana. Food Chem Toxicol 2008;46(2):688-93.

Mohamed GA, Al-Abd AM, El-Halawany AM, Abdallah HM, Ibrahim SR. New xanthones and cytotoxic constituents from Garcinia mangostana fruit hulls against human hepatocellular, breast, and colorectal cancer cell lines. J Ethnopharmacol 2017;198:302-12.

Choudhury B, Kandimalla R, Bharali R, Monisha J, Kunnumakara AB, Kalita K, et al. Anticancer Activity of Garcinia morella on T-cell murine lymphoma via apoptotic induction. Front Pharmacol 2016;7:3.

Shibata MA, Iinuma M, Morimoto J, Kurose H, Akamatsu K, Okuno Y, et al. A mangos tin extracted from the pericarp of the Mangosteen (Garcinia mangostana Linn) reduces Tumor growth and lymph node metastasis in an immunocompetent xenograft model of metastatic mammary cancer carrying a p53 mutation. BMC Med 2011;9:69.

Ahmad A, Sarkar SH, Aboukameel A, Ali S, Biersack B, Seibt S, et al. Anticancer action of garcinol in vitro and in vivo is in part mediated through inhibition of STAT-3 signaling. Carcinogenesis 2012;33(3):2450-6.

Zhou J, Luo YH, Wang JR, Lu BB, Wang KM, Tian Y. Gambogenic acid induction of apoptosis in a breast cancer cell line. Asian Pac J Cancer Prev 2013;14(12):7601-5.

Hallett A, Traunecker H. A review and update on Neuroblastoma. J Paediatr Child Health 2012;22(3):103-7.

Maris JM, Hogarty MD, Bagatell R, Cohn SL. Neuroblastoma. Lancet 2007;369(9579):2106-20.

Sofowara A. Medicinal Plants and Traditional Medicine in Africa. 3rd ed. Ibadan, Nigeria: Spectrum Books Limited; 2008. p. 199-204.

Sridevi M, John B, Yamini K. Anti-cancer effect of Ocium sanctum ethanolic extract on non-small cell lung carcinoma cell. Int J Pharm Pharm Sci 2016;8(4):242-6.

Nazeema BB, Julie J, Abirami J, Kumareasan R, Muthukumaran T, Rajasree S, et al. Anti-cancer activity of Datura metel on mcf-7 cell line. Asian J Pharm Clin Res 2014;7(1):181-3.

Sharma R, Chandan G, Chahal A, Saini RV. Antioxidant and anticancer activity of methanolic extract from Stephania elegans. Int J Pharm Pharm Sci 2017;9(2):245-9.