APPLICATION OF EXTRACTIVE SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCLOXACILLIN AND TRANDOLAPRIL USING BROMOCRESOL GREEN

Authors

  • VIJAYALAKSHMI R
  • SOWJANYA D
  • ARCHANA S
  • DHANARAJU MD

Abstract

Objective: The aim was to develop rapid, simple, sensitive, and accurate visible spectrophotometric methods for the determination of flucloxacillin
and trandolapril.
Materials and Methods: These methods are based on the formation of ion-pair complex of the flucloxacillin, with bromocresol green in acidic
medium. The colored products are extracted with chloroform and measured spectrophotometrically at 433 nm and 430 nm for flucloxacillin and
trandolapril, respectively.
Results: Beer's law was obeyed in the concentration range of 0.5-2.5 mg/ml and 1-5 mg/ml with molar absorptivity of 2.46×102 L/mol/cm and
4.52×102 L/mol/cm and relative standard deviation of 0.28% and 0.32% for flucloxacillin and trandolapril, respectively.
Conclusion: These methods have been successfully applied for the assay of drug in pharmaceutical formulations individually. No interference was
observed from common pharmaceutical adjuvants by adopting the proposed method.

Keywords: Flucloxacillin, Trandolapril, Extractive spectrophotometry, Bromocresol green.

Downloads

Download data is not yet available.

References

Merck Index, an Encyclopedia of Chemicals, Drugs and Biological. 14th

ed. Rahway, New Jersey, U.S.A: Division of Merck and Co., Inc.;

p. 4111.

Gujral RS, Haque SM, Shanker P. A sensitive validated

spectrophotometric method for the determination of flucloxacillin

sodium. E J Chem 2009;6 Suppl 1:S397-405.

Aly HM, Amin AS. Utilization of ion exchanger and spectrophotometry

for assaying amoxycillin and flucloxacillin in dosage form. Int J Pharm

;338(1-2):225-30.

Refat MS, El-Didamony AM. Spectrophotometric and electrical studies

of charge-transfer complexes of sodium flucloxacillin with pi-acceptors.

Spectrochim Acta A Mol Biomol Spectrosc 2006;65(3-4):732-41.

Mohamed GG, Nour El-Dien FA, Eman FU. Spectrophotometric

determination of the reaction mechanism between DDQ as П receptor

and potassium iodate and flucloxacillin and dicloxacillin drugs and

their determination in pure and dosage forms. Spectrochim Acta A Mol

Biomol Spectrosc 2006;65(1):11-9.

El-Dien FA, Mohamed GG, Farag EY. Spectrophotometric

determination of flucloxacillin and dicloxacillin in pure and dosage

forms. Spectrochim Acta A Mol Biomol Spectrosc 2006;64(1):210-5.

El-Mammli MY. Spectrophotometric determination of flucloxacillin in

pharmaceutical preparations using some nitrophenols as a complexing

agent. Spectrochim Acta A Mol Biomol Spectrosc 2003;59(4):771-6.

Mohamed GG. Spectrophotometric determination of ampicillin,

dicluxacillin, flucloxacillin and amoxicillin antibiotic drugs: Ion-pair

formation with molybdenum and thiocyanate. J Pharm Biomed Anal

;24(4):561-7.

Zhou Q, Ruan Z, Yuan H, Jiang B, Xu D. RP-HPLC analysis of

flucloxacillin in human plasma: Validation and application to a

bioequivalence study. Pharmazie 2007;62(2):101-4.

Shanmugasundaram P, Raj KR, Rangan MJ, Devdass G, Arunadevi M,

Maheswari R, et al. Simultaneous estimation of amoxicillin and

flucloxacillin in its combined capsule dosage forms by HPLC. Rasayan

J Chem 2009;2(1):57-60.

Liu H, Wang H, Sunderland VB. An isocratic ion exchange HPLC

method for the simultaneous determination of flucloxacillin and

amoxicillin in a pharmaceutical formulation for injection. J Pharm

Biomed Anal 2005;37(2):395-8.

Charles BG, Foo CC, Gath J. Rapid column liquid chromatographic

analysis of flucloxacillin in plasma on a microparticulate pre-column.

J Chromatogr B Biomed Appl 1994;660(1):186-90.

Martindale the Extra pharmacopoeia. 36th ed. London, UK: Royal

Pharmaceutical Society; 2009. p. 1416.

Gumieniczek A, Hopkała H. High-performance liquid chromatographic

assay of trandolapril in capsules. Acta Pol Pharm 2000;57(4):253-5.

Cendrowska I, Bańkowski K, Iskra-Jopa J. Astudy on the stereochemical

purity of trandolapril and octahydro-1H-indole-2-carboxylic acid by

HPLC method. Acta Pol Pharm 2003;60(2):141-4.

Constantinos P, Maria K, Irene P. Liquid chromatographic tandem mass

spectrometric determination of trandolapril in human plasma. Anal

Chim Acta 2005;540(2):375-82.

Nirogi RV, Kandikere VN, Shrivastava W, Mudigonda K.

Quantification of trandolapril and its metabolite trandolaprilat in

human plasma by liquid chromatography/tandem mass spectrometry

using solid-phase extraction. Rapid Commun Mass Spectrom

;20(24):3709-16.

Kotaiah RM, Ganesh B, Chandra Sekhar KB, Rasheed SH,

Venkateswarlu Y, Dhandapani B. HPTLC method development and

validation for the estimation of trandolapril in bulk and its formulations.

Asian J Res Chem 2010;3(1):158-60.

Kowalczuk D. Simultaneous high-performance thin-layer

chromatography densitometric assay of trandolapril and verapamil in

the combination preparation. J AOAC Int 2005;88(5):1525-9.

Published

01-09-2014

How to Cite

R, V., S. D, A. S, and D. MD. “APPLICATION OF EXTRACTIVE SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCLOXACILLIN AND TRANDOLAPRIL USING BROMOCRESOL GREEN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 4, Sept. 2014, pp. 216-8, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/2113.

Issue

Section

Original Article(s)