STUDYING THE CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF CRUDE EXTRACTS FROM ANACYCLUS NIGELLIFOLIUS BOISS GROWN IN NORTH LEBANON.
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i1.21690Keywords:
Total phenolic, Total flavonoid, Antioxidant, Cytotoxic potentiallyAbstract
Objective: The objective of this study is to evaluate the chemical contents (total phenolic and total flavonoid contents) of the crude ethanolic and aqueous extracts from leaves and stems of a Lebanese plant and to evaluate their cytotoxic and antioxidant activities.
Methods: Quantification of the total phenolic and total flavonoid contents of all extracts of Anacyclus nigellifolius from leaves and stems and the cytotoxic activity were evaluated through XTT assay, while antioxidant activities were evaluated using spectrophotometric analyses DPPH, H2O2, and chelating of ferrous ions tests.
Results: The ethanolic extract from leaves and stem of A. nigellifolius was better than aqueous extract and showed a higher content in total phenolic and total flavonoid which was 39.48±0.04 and 13±0.03, respectively. On the other hand, the best value of IC50 of DPPH, H2O2, and chelating of ferrousions of ethanol extract from leaves was 0.1 mg/mL, 0.25 mg/mL, and 0.37 mg/mL, respectively, also, ethanol extract has significantly inhibited cells growth 293T and MCF7, a percentage of inhibition was in leaves, and it has reached the 70% and 54%, respectively.
Conclusions: Both ethanolic and aqueous extracts of both parts of A. nigellifolius have shown high efficiency and good antioxidant and antiproliferation power. Hence, this plant can be used in the prevention of a number of diseases related to oxidative stress as well as might be used for inhibition of cancer cell line growth.
Downloads
References
Hong H, Liu GQ. Protection against hydrogen peroxide-induced cytotoxicity in PC12 cells by scutellarin. Life Sci 2004;74:2959-73.
Farhan H, Malli F, Rammal H, Hijazi A, Bassal A, Ajouz N, et al. Phytochemical screening and antioxidant activity of Lebanese Eryngium creticum L. Asian Pac J Trop Biomed 2012;2(3):1217-20.
Butler MS. Natural products to drugs: Natural product-derived compounds in clinical trials. Nat Prod Rep 2008;25:475-516.
Tohme G, Tohme H. Illustrated Flora of Lebanon. Beirut Lebanon: National Council for Scientific Research; 2007.
Allali H, Benmehdi H, Dib MA, Tabti B, Ghalem S, Benabadji N. Phytotherapy of diabetes in West Algeria. Asian J Chem 2008;20:2701-10.
Bellakhdar J. La Pharmacopée Marocaine Traditionnelle: Médecine Arabe Ancienne et Savoirs Populaires - Saint-Etienne, Edit. Paris: Ibis Press; 1997. p. 177.
Doudach L, Meddah B, Alnamer R, Chibani F, Cherrah Y. In vitro antibacterial activity of the methanolic and aqueous extracts of Anacyclus pyrethrum used in Moroccan traditional medicine. Int J Pharm Pharm Sci 2012;4:402-5.
Harborne JB. Phytochemical Methods. London: Chapman and Hall, Ltd.; 1973. p. 49-188.
Lister E, Wilson P. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity. New Zealand: Crop Research Institute Lincoln; 2001.
Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum moench) hulls and flour. J Ethnopharmacol 2000;72:35-42.
Chew YL, Goh JK, Lim YY. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from leguminosae family in Peninsular Malaysia. Food Chem 2009;116:13-8.
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.
Dinis TC, Maderia VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161-9.
Weislow OS, Kiser R, Fine DL, Bader J, Shoemaker RH, Boyd MR, et al. New soluble-formazan assay for HIV-1 cytopathic effects: Application to high-flux screening of synthetic and natural products for AIDS-antiviral activity. J Natl Cancer Inst 1989;81:577-86.
Hijazi A, Sabbah A, Falah AS, Zeiter S, Rammal H, Nasser M. Antioxidant, antiproliferative properties and chemical composition of the ethanolic extract from leaves and stems of Lebanese Anacyclus nigellifolius Boiss. J Adv Med Pharm Sci 2016;11:1-9.
Sujith K, Darwin CR, Suba V. Antioxidant activity of ethanolic root extract of Anacyclus pyrethrum. Int Res J Pharm 2011;2:222-6.
Sun J, Chu YF, Wu X, Liu RH. Antioxidant and ant proliferative activities of common fruits. J Agric Food Chem 2002;50:7449-54.
Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157-84.
Dasilva LI, Karuppusamy A, Miyajima F, Violantei MP, Bieski IG, Balogun SO, et al. Antimicrobial and antioxidant activities of selected plants used by populations from Juruena valley, legal amazon. Int J Pharm Pharm Sci 2017;9:179-91.
Farhan H, Rammal H, Hijazi A, Daher A, Reda M, Annan H, et al. Chemical composition and antioxidant activity of a Lebanese plant euphorbia macroclada schyzoceras. Asian Pac J Trop Biomed 2013;3:542-8.
Farhan H, Rammal H, Hijazi A, Annan H, Daher A, Reda M, et al. Chemical composition, in vitro cytotoxicity and anti-free radical properties of six extracts from Lebanese Trigonella berythea boiss. Pak J Pharm Sci 2013;26:1157-63.
Farhan H, Rammal H, Hijazi A, Hamad H, Daher A, Reda M, et al. In vitro antioxidant activity of ethanolic and aqueous extracts from crude Malva parviflora L. grown in Lebanon. Asian J Pharm Clin Res 2012;5:234-8.
Bendjeddou D, Lalaoui K, Satta D. Immunostimulating activity of the hot water-soluble polysaccharide extracts of Anacyclus pyrethrum, Alpinia galanga and Citrullus colocynthis. J Ethnopharmacol 2003;88:155-60.
Krishnaraju AV, Rao TV, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. International assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. J Appl Sci Eng 2005;3:125-34.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
