FORMULATION AND EVALUATION OF NOVEL ANTIBACTERIAL AND ANTI-INFLAMMATORY CREAM CONTAINING MUNTINGIA CALABURA LEAVES EXTRACT
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i12.21963Keywords:
Muntingia calabura, Kerukup Siam, Disc diffusion method, Albumin denaturation technique, Antibacterial, Anti-inflammatoryAbstract
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 Objective: The main objective of the present study was to formulate antibacterial and anti-inflammatory cream using Muntingia calabura leaves extract.
Methods: 500 g of matured leaves of M. calabura were extracted with 70% methanol using maceration method for a week. The antibacterial activity of the extract and formulated cream in different concentration was investigated against two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) through disc diffusion method. The anti-inflammatory activity of extract and formulated cream was studied using inhibition of albumin denaturation technique.
Results: The antibacterial activity of the methanolic extract was studied in different concentrations (5, 10, and 25 mg/ml) and the formulated cream was studied in the concentration of (10, 25, 50, and 100 mg/ml). The extract showed significant antibacterial activity with all the tested pathogens with a zone of inhibition ranges from 3.33 ± 0.88-14.60 ± 0.80 mm. As for the formulated cream, the zone of inhibition for F1 ranged from 0.67 ± 0.67 mm to 14.80 ± 1.17 mm. F2 presented zone of inhibition at 3.40 ± 1.64 mm to 15.00 ± 1.18 mm. The anti-inflammatory activity showed maximum inhibition of 63.93 ± 5.90, 41.83 ± 5.64, and 27.20 ± 2.72 was observed at 1000 μg/ml for the extract, F2 and F1, respectively. The cream was found to be in stable state after 2 months.
Conclusion: The results showed that the formulated cream can be used for bacterial infections and to reduce inflammation. We recommend this formulation need to be further developed into commercial standards.
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References
Buhian WP, Rubio RO, Valle DL, Martin-Puzon JJ. Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. Leaves and stems. Asian Pac J Trop Biomed 2016;6(8):682-5.
Ullah HM, Zaman S, Juhara F, Akter L, Mohammed TS, Masum EH, et al. Evaluation of antinociceptive, in-vivo and in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complement Altern Med 2014;14:1-12.
Ziebrou M, Lompo M, Ouedraogo N, Yaro B, Guissoun I. Antioxidant, analgesic and anti-inflammatory activities of the leafy stems of Waltheria indica L. (Sterculiaceae). J Appl Pharm Sci 2016;6(11):124-9.
Yusof MM, Salleh M, Kek TL, Ahmat N, Azmin NN, Zakaria Z. Activity-guided isolation of bioactive constituents with antinociceptive activity from Muntingia calabura L. Leaves using the formalin test. Hindawi Publ Corp 2013;2013:1-9.
Vijayanand DS, Thomas AS. Screening of Michelia champacca and Muntingia calabura extracts for potential bioactives. Int J Pharm Sci Res 2016;7(6):266-73.
Zakaria ZA, Balan T, Azemi AK, Omar MH, Mohtarrudin N, Ahmad Z, et al. Mechanism(s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calabura. BMC Complement Altern Med 2016;16(1):78.
Zakaria ZA, Sani MH, Cheema MS, Kader AA, Kek TL, Salleh MZ. Antinociceptive activity of methanolic extract of Muntingia calabura leaves: Further elucidation of the possible mechanisms. BMC Complement Altern Med 2014;14:1-12.
Purushothaman RS, Guruswamy P, Sabesan G. Synergistic effect of anti-oxidant, anti-tyrosinase and anti-bacterial activities of Tridax procumbens, Lantana camara, Euphorbia hirta and Thevetia peruviana plant extracts for cosmetic and personal care applications. Int J Pharm Pharm Sci 2014;6:91-4.
Mahendran S, Ting CP, Syafiq AM, Nalina K. Comparative evaluation of antimicrobial properties of red and white ginger. Asian J Pharm Clin Res 2014;7(1):108-10.
Mahendran S, Pavitra S, Afzan M. Formulation and evaluation of novel antiaging cream containing rambutan fruit extract. Int J Pharm Sci Res 2017;8:1000-10.
Mahendran S, Nurashikin AR. Formulation, evaluation and antibacterial properties of herbal ointment containing methanolic extract of Clinacanthus nutans leaves. Int J Pharm Clin Res 2016;8(8):1170-4.
Halima S, Rachida A, Fatima ZE. Antioxidant and antibacterial activities of six Algerian medicinal plants. Int J Pharm Pharm Sci 2016;8(1):364-7.
Leelaprakash G, Das SM. In-vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3(3):189-96.
Kamazeri T, Samah OA, Taher M, Susanti D, Qaralleh H. Antimicrobial activity and essential oils of Curcuma aeruginosa, Curcuma mangga, and Zingiber cassumunar from Malaysia. Asian Pac J Trop Med 2012;5(3):202-9.
Li XZ, Plesiat P, Nikaido H. The challenge of efflux-mediated antibiotic resistance in gram-negative bacteria. Clin Microbiol Rev 2015;28(2):337-418.
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