PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i1.21999Keywords:
Psidium guajava, Silver nanoparticles, Fourier transform infrared, Transmission electron microscopy, Antibacterial activityAbstract
 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.
Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.
Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.
Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.
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Senol AM, Metin O, Acar M, Onganer Y, Meral K. The interaction of fluorescent Pyronin Y molecules with monodisperse silver nanoparticles in chloroform. J Mol Struct 2016;1103:212-6.
Sathish S, Shekar BC, Nataraj D. Synthesis and characterization of indium aluminate (InAlO3) nanoparticles by wet chemical method. Adv Powder Tech 2014;25:1007-15.
Darder M, Colilla M, Ruiz-Hitzky E. Citosan-clay nanocomposites: Applications as electrochemical sensors. App Clay Sci 2005;28:199-208.
Nair LS, Laurencin CT. Silver nanoparticles: Synthesis and therapeutic applications. J Biomed Nanotech 2007;3:301-16.
Chou CS, Chen CY, Lin SH, Lu WH, Wu P. Preparation of TiO2/bamboo-charcoal-powder composite particles and their applications in dye-sensitized solar cells. Adv Powder Tech 2015;26:711-7.
Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against Gram-positive and Gram-negative bacteria. Nanomed Nanotech Bio Med 2012;8:37-45.
Bian X, Lu X, Jin E, Kong L, Zhang W, Wang C, et al. Fabrication of PT/polypyrrole hybrid hollow microspheres and their application in electrochemical biosensing towards hydrogen peroxide. Talanta 2010;81:813-8.
Banerjee A, Theron R, Scott RW. Redispersion of sinthered nanoparticles catalysts in tetraalkylphosphonium ionic liquid. J Mol Catal Chem 2014;393:105-11.
Peng S, McMahon JM, Schatz GC, Gray SK, Sun Y. Reversing the size-dependence of surface plasmon resonances. Proc Natl Acad Sci U S A 2010;107:14530-4.
Sabatini CA, Pereira RV, Gehlen MH. Fluorescence modulation of acridine and coumarin dyes by silver nanoparticles. J Fluores 2007;17:377-82.
Setua P, Ghatak C, Rao VG, Das SK, Sarkar N. Dynamics of solvation and rotational relaxation of coumarin 480 in pure aqueous-AOT reverse micelle and reverse micelle containing different-sized silver nanoparticles inside its core: A comparative study. J Phys Chem 2012;116:3704-12.
Solomon MM, Umoren SA. In-situ preparation, characterization and anticorrosion property of polypropylene glycol/silver nanoparticles composite for mild steel corrosion in acid solution. J Colloid Interface Sci 2016;462:29-41.
Nia PM, Meng WP, Alias Y. Hydrogen peroxide sensor: Uniformly decorated silver nanoparticles on polypyrrole for wide detection range. App Surf Sci 2015;357:1565-72.
Deshpande R, Ravishankar B, Sharanbasava G, Mahesh DB, Harsoor V, Yalagatti MS, et al. Anti-cancer studies of noble metal nanoparticles synthesized using different plant extracts. Cancer Nanotech 2011;2:57-65.
Ragunathan D, Basavaraj S, Sawle B, Manjunath SY. Biosynthesis of stable polyshaped gold nanoparticles from microwave-exposed aqueous extracellular anti-malignant guava (Psidium guajava) leaf extract. Nanobiotechnology 2009;5:34-41.
Teixeira RS, Camparoto ML, Mantovani MS, Vicentini VE. Assesment of two medicinal plants, Psidium guajava L. and Achillea millefolium. L in vitro and in vivo assays. Genet Mol Biol 2003;26:234-9.
Ojewolw JE. Anti inflamatory and analgesic effects of Psidium guajava Linn. (Myrtacaea) leaf aqueous extract in rats and mice. Met Fin Exp Clini Pharma 2006;28:441-6.
Bang LS, Ryong PH. Anticancer activity of guava (Psidium guajava l.) branch extract against HT-29 human colon cancer cells. J Med Plant Res 2010;4:891-6.
Ramadhania ZM, Insanu M, Gunarti NS, Wirasutisna KR, Sukrasno S, Hartati R. Antioxidant activity from ten species of Myrtaceae. Asian J Pharm Clin Res 2017;5-7.
Parashar UK, Kumar V, Bera T, Saxena PS, Nath G, Srivastava SK, et al. Study of mechanism of enhanced antibacterial activity by green synthesis of silver nanoparticles. Nanotechnology 2011;22:415104.
Xiao-Long Y, Kun-Lung H, Ji-Kai L. Guajadial: An unusual metroterpenoid from guava leaves Psidium guajava. Org Lett 2007;9:5135-8.
Gutierrez RM, Mitchell S, Solis RV. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharma 2008;117:1-27.
Deshpande R, Bedre DM, Basavaraja S, Balaji SD, Manjunath SY, Venkataraman A, et al. Microwave-assisted rapid extracellular synthesis of stable bio-functionalized silver nanoparticles from guava (Psidium guajava) leaf extract. J Nanopart Res 2011;13:2021 8.
Shirur DS, Adhikari R, Sanjay ST, Sajjekhan S. A review on the medicinal plant Psidium guajava. J Drug Deliv Ther 2013;3:162-8.
Rajamani R, Kuppusamy S, Shamugavadivu M. Green synthesis of silver nanoparticles using areca nut extract for enhanced antibacterial activity. J Green Sci Technol 2013;1:102-6.
Sharmila C, Vinuppriya R, Chandran S, Chemmanda J, Chandarsheka B. Biosynthesis of PVA encapsulated silver nanoparticles. J App Res Tech 2016;14:319-24.
MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B Biointerfaces 2011;85:360-5.
Tanakae T, Ishida N, Ishimatsu M, Nonaka G, Nishioka I. Tannins and related compounds CXVI. Tannins, guajavins psidinins and psiguavin from the bank of Psidium guajava leaf. Chem Pharm Bull 1992;40:2092-8.
Jayeeta B, Neeraj ST, Kirar S, Banerjee UC. Bioinspired nanotheranostic agents: Synthesis, surface functionalization, and antioxidant potential. ACS Biomater Sci Eng 2015;1:382-92.
Bankura KP, Maity D, Mollick MM, Mondal D, Bhowmick B, Bain MK, et al. Synthesis, characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium. Carbohydr Polym 2012;89:1159-65.
Ragunath A, Nisha P, Kumuthakalavalli K. Mycosynthesis of silver nanoparticles: Characterization, antioxidant and anti-inflammatory activity from Pleurotus florida (Mont) singer: A macro fungi. Asian J Pharm Clin Res 2017;10:186-91.
Coseri S, Spatareanu A, Sacarescu L, Rimbu C, Suteu D, Spirk S, et al. Green synthesis of the silver nanoparticles mediated by pullulan and 6-carboxypullulan. Carbohydr Polym 2015;116:9-17.
Kirthika P, Dheeba B, Sivakumar R, Abdulla SS. Plant mediated synthesis and characterization of silver nanoparticles. Int J Pharm Pharm Sci 2014;6:304-10.
Mittal AK, Bhaumik J, Kumar S, Banerjee UC. Biosynthesis of silver nanoparticles: Elucidation of prospective mechanism and therapeutic potential. J Coll Int Sci 2014;415:39-47.
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. Coli as a model for gram-negative bacteria. J Colloid Interface Sci 2004;275:177-82.
Murugesan S, Bhuvaneshwari S, Sivamurugan V. Green synthesis characterization of silver nanoparticles of marine red alga spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;5:192-7.
Sengodan R, Dinesh KP, Ranjithkumar R, Sagadevan P, Sathish S, Chandar Shekar B. Synthesis, characterization and remedial aspect of BaTiO3 nanoparticles against bacteria. Nanomed Nanobiotech 2014; 1:1-5.
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