EFFECT OF FORCED EXERCISE (TAP STRESS) ON DROSOPHILA MELANOGASTER AND EFFICACY OF ABHRAK BHASMA AS DIETARY SUPPLEMENT.
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i1.22139Keywords:
Drosophila, Physical stress, Abhrak bhasma, Ascorbic acidAbstract
 Objective: Generation of reactive oxygen species and other oxidants as an outcome of aerobic respiration has been well documented. During boosted muscular activities/exercise, there is an increase in the rate of respiration and subsequent generation of oxidants; for which, organisms possess various defense systems. There is, however, paucity of reports on the effect of forced exercise in Drosophila melanogaster.
Methods: The present investigation reports a method for inducing forced exercise, the tap stress,†in D. melanogaster. This method takes advantage of the natural tendency of Drosophila flies to climb/fly upward in a cylindrical tube. Simplicity of assembly, ease of experimentation and cost effectiveness are some of the key advantages of this method. Tap stress has been assumed to manifest through the generation of oxidative stress, thus, parameters indicative of oxidative stress have been evaluated.
Results: The results indicate that tap stress induces oxidative stress mainly by influencing the non-catalytic components; total reduced glutathione (GSH), GSH: oxidized glutathione ratio, and free radical scavenging capacity. The catalytic components (superoxide dismutase, and catalase), on the contrary, remain largely unaffected. Abhrak bhasma (AB) influences total GSH content with an increase by about 38±21%, while flies on control diet register a decrease in total GSH content by 34±7% during tap stress. Feeding flies on AB, specifically, show a prominent increase in the transcription levels of cap n†collar C, heat shock protein 70 and catalase genes (>1.5 fold).
Conclusion: Overall, dietary supplement of AB and ascorbic acid benefits Drosophila flies in the management of oxidative stress induced through tap stress.
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Warburton DE, Nicol CW, Bredin SS. Prescribing exercise as preventive therapy. Can Med Assoc J 2006;174:961-74.
Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 5th ed. Oxford: Oxford University Press; 2015.
Davison GW. Exercise and oxidative damage in nucleoid DNA quantified using single cell gel electrophoresis: Present and future application. Front Physiol 2016;7:249.
Novak CM, Burghardt PR, Levine JA. The use of a running wheel to measure activity in rodents: Relationship to energy balance, general activity, and reward. Neurosci Biobehav Rev 2012;36:1001-14.
Watson J. Oxidants, antioxidants and the current incurability of metastatic cancers. Open Biol 2013;3:120144.
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed Res Int 2014;2014:761264.
Meister A. Selective modification of glutathione metabolism. Science 1983;220:472-7.
Menni C, Metrustry SJ, Mohney RP, Beevers S, Barratt B, Spector TD, et al. Circulating levels of antioxidant vitamins correlate with betterlung function and reduced exposure to ambient pollution. Am J Respir Crit Care Med 2015;191:1203-7.
Silver JT, Noble EG. Regulation of survival gene hsp70. Cell Stress Chaperones 2012;17:1-9.
Brewer MS. Natural antioxidants: Sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf 2011;10:221-47.
Chand K, Rajeshwari, Hiremathad A, Singh M, Santos MA, Keri RS. A review on antioxidant potential of bioactive heterocycle benzofuran: Natural and synthetic derivatives. Pharmacol Rep 2017;69:281-95.
Rutkowski M, Grzegorczyk K. Adverse effects of antioxidative vitamins. Int J Occup Med Environ Health 2012;25:105-21.
Dueñas-GarcÃa IE, Heres-Pulido ME, Arellano-Llamas MR, De la Cruz-Núñez J, Cisneros-Carrillo V, Palacios-López CS, et al. Lycopene, resveratrol, vitamin C and FeSO4 increase damage produced by pro-oxidant carcinogen 4-nitroquinoline-1-oxide in Drosophila melanogaster: Xenobiotic metabolism implications. Food Chem Toxicol 2017;103:233-45.
Massie HR, Aiello VR, Doherty TJ. Dietary vitamin C improves the survival of mice. Gerontology 1984;30:371-5.
Pallauf K, Bendall JK, Scheiermann C, Watschinger K, Hoffmann J, Roeder T, et al. Vitamin C and lifespan in model organisms. Food Chem Toxicol 2013;58:255-63.
Dwivedi V, Anandan EM, Mony RS, Muraleedharan TS, Valiathan MS, Mutsuddi M, et al. In vivo effects of traditional ayurvedic formulations in Drosophila melanogaster model relate with therapeutic applications. PLoS One 2012;7:e37113.
Subedi RP, Vartak RR, Kale PG. Effect of abhrak bhasma on the physiology and behaviour of Drosophila melanogaster. Int J Adv Res 2017;5:1629-41.
Aebi HE. Catalase. In: Method of Enzymatic Analysis. Vol. 3. Germany, Weinheim, FL: VCH, Germany-Deerfield; 1983. p. 273-86.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.
Rahman I, Kode A, Biswas SK. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc 2006;1:3159-65.
Brand-williams W, Cuvelier ME, Berset C. Use of free radical methodto evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.
Zeb A, Ullah F. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. J Anal Methods Chem 2016;2016:9412767.
Devasagayam TP, Boloor KK, Ramasarma T. Methods for estimating lipid peroxidation: An analysis of merits and demerits. Indian J Biochem Biophys 2003;40:300-8.
Musarò A, Fulle S, Fanò G. Oxidative stress and muscle homeostasis. Curr Opin Clin Nutr Metab Care 2010;13:236-42.
Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomolecules 2015;5:356-77.
Jennings BH. Drosophila-a versatile model in biology and medicine. Mater Today 2011;14:190-5.
Beckingham KM, Armstrong JD, Texada MJ, Munjaal R, Baker DA. Drosophila melanogaster - the model organism of choice for the complex biology of multi-cellular organisms. Gravit Space Biol Bull 2005;18:17-29.
Nichols CD, Becnel J, Pandey UB. Methods to assay Drosophila behavior. J Vis Exp 2012;. pii: 3795.
Piazza N, Gosangi B, Devilla S, Arking R, Wessells R. Exercise-training in young Drosophila melanogaster reduces age-related decline in mobility and cardiac performance. PLoS One 2009;4:e5886.
Le Bourg E. Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Lett 2001;498:183-6.
Durga M, Nathiya S, Devasena T. In vitro evaluation of cytotoxicity, oxidative stress, DNA damage and inflammation induced by diesel exhaust particles in human a549 lung cells and murine raw 264.7 macrophages. Int J Pharm Pharm Sci 2014;6:105-10.
Noctor G, Foyer CH. Ascorbate and glutathione: Keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 1998;49:249-79.
Dasari P, Anandamurali R, Nayak P. Effect of tocotrienol pretreatment on ex vivo superoxide and peroxide handling capacities (sphc) of rat serum and brain. Int J Pharm Pharm Sci 2017;9:116-22.
Bryan HK, Olayanju A, Goldring CE, Park BK. The Nrf2 cell defence pathway: Keap1-dependent and-independent mechanisms of regulation. Biochem Pharmacol 2013;85:705-17.
Doganlar O, Doganlar ZB. Responses of antioxidant enzymes and heat shock proteins in Drosophila to treatment with a pesticide mixture. Arch Biol Sci 2015;67:869-76.
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