THE ADDITIVITY ANTINOCICEPTIVE INTERACTIONS BETWEEN DICLOFENAC AND THE DERRIS SCANDENS EXTRACT DRUG IN MICE.

Tadsanee Punjanon

doi:10.22159/ajpcr.2018.v11i1.22316

Authors

Tadsanee Punjanon Department of Medical Science, Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum-thani 12000, Thailand.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i1.22316

Keywords:

Derris scandens, Diclofenac, Analgesic effect, Mouse, Abdominal constriction test

Abstract

Â Objective: Combination therapy is a valid approach in pain treatment, in which a reduction of doses could reduce side effects and still achieve optimal analgesia. The objective was to determine the effects of coadministered diclofenac and the Derris scandens extract drug.

Methods: Acetic acid-induced abdominal constriction test in mice was used to determine the type of interaction between components. The effective dose that produced 50% antinociception (ED50) was calculated from the log dose-response curves of fixed ratio combinations of diclofenac with the D. scandens extract drug. The ED50 was compared to the theoretical additive ED50 calculated from the ED50 of diclofenac and of the D. scandens extract drug alone.

Results: Diclofenac and the D. scandens extract drug doseâ€dependently and significantly reduced the abdominal writhing. The combination was the additive effect, the experimental ED50 being smaller than the theoretically calculated ED50. Interaction index of the combination was 0.89.

Conclusion: The present study demonstrates the additivity antinociceptive interactions between diclofenac and the D. scandens extract drug and may be used as a combination analgesic in the treatment of pain conditions.

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Author Biography

Tadsanee Punjanon, Department of Medical Science, Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum-thani 12000, Thailand.

Pharmacology and Toxicology Unit
Department of Medical Sciences
Faculty of Science

References

Laupattarakasem P, Houghton PJ, Hoult JR, Itharat A. An evaluation of the activity related to inflammation of four plants used in Thailand to treat arthritis. J Ethnopharmacol 2003;85:207-15.

Ganapathy S, Josaphine JS, Thomas PS. Anti-inflammatory activity of Derris scandens. J Neurosci Res 2006;6:73-6.

Thailand National List of Essential Medicines; 2016. p. 244-5. Available from: http://www.drug.fda.moph.go.th:81/nlem.in.th. [Last retrieved on 2017 Feb 01].

Srimongkol Y, Warachit P, Chavalittumrong P, Sriwanthana B, Pairour R, Inthep C, et al. A study of the efficacy of Derris scandens (Roxb.) Benth. extract compared with diclofenac for the alleviation of low back pain. J Thai Tradit Altern Med 2007;5:17-23.

Kuptniratsaikul V, Pinthong T, Bunjob M, Thanakhumtorn S, Chinswangwatanakul P, Thamlikitkul V. Efficacy and safety of Derris scandens Benth extracts in patients with knee osteoarthritis. J Altern Complement Med 2011;17:147-53.

The Government Public Relations Department. Promoting Thai Herbal Medicine and Health Behavior Change Program. Available from: http://www.thailand.prd.go.th/ewt_news.php?nid=2141&filename=index. [Last retrieved on 2016 Feb 01].

Raffa RB. Pharmacology of oral combination analgesics: Rational therapy for pain. J Clin Pharm Ther 2001;26:257-64.

van Laar M, Pergolizzi JV Jr, Mellinghoff HU, Merchante IM, Nalamachu S, Oâ€™Brien J, et al. Pain treatment in arthritis-related pain: Beyond NSAIDs. Open Rheumatol J 2012;6:320-30.

Koster P, Anderson M, DeBeer EJ. Acetic acid analgesic screening. Fed Proc 1959;18:412-8.

Jain NK, Kulkarni SK. Antinociceptive and anti-inflammatory effects of Tanacetum parthenium L. extract in mice and rats. J Ethnopharmacol 1999;68:251-9.

Tallarida RJ. Drug synergism: Its detection and applications. J Pharmacol Exp Ther 2001;298:865-72.

Prabhu VV, Nalini G, Chidambaranathan N, Kisan SS. Evaluation of anti-inflammatory and analgesic activity of Tridax Procumbens. Linn against formalin, acetic acid and CFA induced pain models. Int J Pharm Pharm Sci 2011;3:126-30.

Benhouda A, Yahia M. Toxicity, analgesic and anti-pyretic activities of methanolic extract from Hyoscyamus Albusâ€™ leaves in albinos rats. Int J Pharm Pharm Sci 2014;6:121-7.

Punjanon T, Yingyong W, Untharin N. Analgesic Synergy Between Paracetamol and Derris scandens in Mice. Proceeding of RSU International Research Conference; 2017. p. 20-4.

Curatolo M, Sveticic G. Drug combinations in pain treatment: A review of the published evidence and a method for finding the optimal combination. Best Pract Res Clin Anaesthesiol 2002;16:507-19.

Vane JR. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol 1971;231:232-5.

Bonnefont J, Courade JP, Alloui A, Eschalier A. Antinociceptive mechanism of action of paracetamol. Drugs 2003;63:1-4.

Sprott H, Shen H, Gay S, Aeschlimann A. Acetaminophen may act through beta endorphin. Ann Rheum Dis 2005;64:1522.

Laupattarakasem P, Houghton PJ, Hoult JR. Anti-inflammatory isoflavonoids from the stems of Derris scandens. Planta Med 2004;70:496-501.

Puttarak P, Sawangjit R, Chaiyakunapruk N. Efficacy and safety of Derris scandens (Roxb.) Benth. for musculoskeletal pain treatment: A systematic review and meta-analysis of randomized controlled trials. J Ethnopharmacol 2016;194:316-323.

Modi CM, Mody SK, Patel HB, Dudhatra GB, Kumar A, Avale M. Toxicopathological overview of analgesic and anti-inflammatory drugs in animals. J Appl Pharm Sci 2014;2:149-57.