SCREENING ANTIMICROBIAL POTENTIAL FOR MALAYSIAN ORIGINATED TAMARINDUS INDICA ETHANOLIC LEAVES EXTRACT

Sridevi Chigurupati; Eric Wong Kwang Yiik; Jahidul Islam Mohammad; Shantini Vijayabalan; Kesavanarayanan Krishnan Selvarajan; Venkata Ramana Reddy M; Sitansu Sekhar Nanda

doi:10.22159/ajpcr.2018.v11i3.22614

Authors

Sridevi Chigurupati Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia.
Eric Wong Kwang Yiik Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia.
Jahidul Islam Mohammad Department of Pharmacology, Faculty of Medicine, Cyberjaya University College of Medical Sciences, CUCMS, Cyberjaya, 63000, Malaysia.
Shantini Vijayabalan Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia.
Kesavanarayanan Krishnan Selvarajan Department of Pharmacology & Toxicology, College of Pharmacy, University of Hail, Hail, Kingdom of Saudi Arabia.
Venkata Ramana Reddy M Department of Pharmaceutical Chemistry, Gurunanak Institutions Technical Campus, School of Pharmacy, JNTU, Hyderabad, India.
Sitansu Sekhar Nanda Department of Chemistry, Myongji University, Yongin, South Korea.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i3.22614

Keywords:

Tamarindus indica, Qualitative analysis, Quantitative analysis, Antimicrobial

Abstract

Â Objective: The aim of the research is to study the antimicrobial potential of Tamarindus indica leaves extract (Malaysian origin).

Methods: T. indica leaves extraction was carried out by maceration (TIME) and Soxhlet extraction methods (TISE). The phytochemical test was conducted for the confirmation of various phytoconstituents present in the leaves extract. Antimicrobial assays were carried out by minimum bactericidal concentration (MBC), minimum inhibitory concentration (MIC), and agar well diffusion method against ATCC bacterial strains.

Results: Ethanolic extraction of T. indica leaves by maceration method showed better yield (70.38%) compared to Soxhlet extraction method (60.55%). The qualitative phytochemical test on TISE and TIME were confirmed the presence of alkaloids, flavonoids, phenols, mucilage, tannins, steroids, proteins, and carbohydrates. TIME was selected for antimicrobial studies, and it was found to have MBC value of 500 Î¼g/mL and MIC value of 250 Î¼g/mL among various test bacteria, and it was also found to exhibit good zone of inhibition against Gram-negative bacteria such as Escherichia coli and Neisseria gonorrhoeae.

Conclusion: These findings deduced that T. indica leaves have various phytochemical constituents which could be responsible for their natural antibacterial activity especially against Gram-negative bacteria.

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