FORMULATION AND CHARACTERIZATION OF PERIODONTAL FILMS CONTAINING AZITHROMYCIN AND SERRATIOPEPTIDASE

Soniya Rani; Nardev Singh

doi:10.22159/ajpcr.2018.v11i5.23032

Authors

Soniya Rani Department of Pharmaceutics, Uttaranchal Instit ute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, Uttarakhand, India. http://orcid.org/0000-0003-3631-0097
Nardev Singh Department of Pharmaceutics, Uttaranchal Instit ute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, Uttarakhand, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i5.23032

Keywords:

Periodontal films, Azithromycin and serratiopeptidase, Solvent casting method, keshary-chien diffusion cell

Abstract

Objective: The objective of the present work was to formulate and evaluate periodontal film, which could be capable of delivering therapeutic concentration of azithromycin and serratiopeptidase for a prolonged period of time and could be easily placed into the periodontal pocket.

Methods: The films were prepared by solvent casting method using combinations of ethyl cellulose, hydroxypropyl methylcellulose K4M, hydroxypropyl methylcellulose 50 cps, eudragit L-100, and Chitosan in different ratios using dibutyl phthalate as plasticizer. The periodontal films were evaluated for weight variation, thickness, percentage moisture absorption, percentage moisture loss, folding endurance, percentage swelling index, percentage elongation, and in vitro percentage cumulative drug-enzyme release profile.

Results: Formulation F12 was found to be a good periodontal film. Hence, it was considered as an optimized formulation. In vitro drug-enzyme release rate studies using keshary-chien diffusion cell showed maximum drug release in F12 formulation (95.92% for azithromycin and 94.20% for serratiopeptidase at the end of 24 h) compared to other formulations.

Conclusion: The optimized formulation F12 showed the best drug-enzyme release profile among the others for the preparation of periodontal film. There is a scope for the further study and development of the azithromycin and serratiopeptidase periodontal films.

Downloads

Download data is not yet available.

Author Biography

Soniya Rani, Department of Pharmaceutics, Uttaranchal Instit ute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, Uttarakhand, India.

Pharmaceutics

References

Preeti SK, Mahendra DK. Ciprofloxacin HCl loaded cubic phase gel for periodontal intrapocket administration. Res J Pharm Biol Chem Sci 2012;3:869-70.

Kornman KS. Controlled-release local delivery antimicrobials in periodontics: Prospects for the future. J Periodontol 1993;64:782-91.

Dodwad V, Vaish S, Chhokra M, Mahajan A. Magic bullet to treat periodontitis-a targeted approach. J Pharm Biomed Sci 2012;20:1-2.

Slots J, Rams TE. Microbiology of periodontal disease. In: Slots J, Taubman MA, editros. Contemporary Oral Microbiology and Immunology. St. Louis: CV Mosby Co.; 1992. p. 425-43.

Newman MG, Takei HH, Klokkevold PR, Carranza FA. Clinical Periodontal. 10th ed. Elsevier, St Louis (MO); 2006 . p. 232-5.

Vandana G, Umesh L, Neetin D. A quick hplc method for quantification of serratiopeptidase from Serratia marcescens using. J Pharm Res 2016;6:54-60.

Vineetha VC, Maria M. Maths development and evaluation of dental films containing an antibacterial agent for the treatment of periodontitis. Int J Pharm Pharm Sci 2014;7:52-9.

Magnusson I, Lindhe J, Yoneyamma T, Lilzenberg B. Recolonization of subgingival microbials following scaling in deep pockets. J Clin Periodontal 1984;11:193-207.

Esposito E, Cortesi R, Cervellati F, Menegatti E, Nastruzzi C. Biodegradable microparticles for sustained delivery of tetracycline to the periodontal pocket-formulary and drug release studies. J Microencapsul 1997;14:175-87.

Rahman S, Ahuja A, Ali J, Khar RK. Site specific delivery systems for the treatment of periodontitis. Indian J Pharm Sci 2003;65:106-12.

Yassin GE, Abass HA. Design and evaluation of fast dissolving oro-dispersible films of metoclopramide hydrochloride using 32 multifactorial designs. Int J Pharm Pharm Sci 2016;8:218-22.

Nair SC, Anoop KR. Design and in vitro evaluation of controlled release satranidazole subgingival films for periodontitis therapy. Int J Pharm Sci Rev Res 2014;24:8-14.

Manubolu K, Chandana AV, Prakash P. Formulation and in vitro characterizations of amitriptyline buccal films. World J Pharm Pharm Sci 2014;3:1547-55.

Mona SA, Semalty KG. Formulation and characterization of mucoadhesive buccal films of glipizide. Indian J Pharm Sci 2008;704:3-8.

Prashant M, Satturwar S, Fulzele V, Avinash K. Dorle evaluation of polymerized rosin for the formulation and development transdermal drug delivery systems. AAPS Pharmscitech 2005;6:48-53.

Fulzele SV, Sattuwar PM, Dorle AK. Polymerized rosin: Novel film forming polymer for drug delivery. Int J Pharm 2002;249:175-84.

Nakahara T, Nakamura T, Kobayashi E, Inoue M, Shigeno K. Novel approach to regeneration of periodontal tissues based on in situ tissue engineering: Effects of controlled release of basic fibroblast growth factor from a sandwich membrane. Tissue Eng 2003;9:153-62.

Renvert S, Lessem J, Dahle NG, Lindahl C, Svensson M. Topical minocycline microspheres versus topical chlorhexidine gel as an adjunct to mechanical debridement of incipient periimplant infections: A randomized clinical trial. J Clin Periodontol 2006;33:362-9.

Salvi GE, Mombelli A, Mayfield L, Rutar A, Suvan J, Garrett S, et al. Local antimicrobial therapy after initial periodontal treatment. J Clin Periodontol 2002;29:540-50.

Minabe M. Application of a local drug delivery system to periodontal therapy. I. Development of collagen preparation with immobilized tetracycline. J Periodontal 1989;60:113-7.

Chadha VS, Arora K, Manjunath BC, Kalra S. Local drug delivery in periodontics-current concepts and trends. Int J Adv Res Oral Sci 2012;1:1-9.