ANALYSIS ON THE INHIBITION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS BY STREPTOCOCCUS SALIVARIUS ISOLATED FROM SALIVA AND TONGUE DORSUM OF ADULTS

Authors

  • Regina Vaniabella Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Hedijanti Joenoes Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Boy M Bachtiar Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23081

Keywords:

Streptococcus salivarius, Aggregatibacter actinomycetemcomitans, Lantibiotic, Probiotic

Abstract

Objective: To analyze the inhibitory potential of S. salivarius isolated from saliva and the dorsum of the tongue, and the protein it produces, ininhibiting the growth of Aggregatibacter actinomycetemcomitans.

Methods: Examine the inhibition zone of A. actinomycetemcomitans formed by the treatment of S. salivarius isolated from saliva and the tonguedorsum, along with the protein produced, in 10 participants using a deferred antagonism test with well-diffused gelatin.

Results: The inhibitory zone of S. salivarius isolated from saliva and the tongue against A. actinomycetemcomitans is insignificantly different (p=0.05).There is no inhibition of the growth of A. actinomycetemcomitans shown by whole-cell and spent medium proteins of S. salivarius.

Conclusion: S. salivarius isolated from both saliva and the tongue dorsum is able to inhibit the growth of A. actinomycetemcomitans, but not theprotein they produce.

Downloads

Download data is not yet available.

References

Horz HP, Meinelt A, Houben B, Conrads G. Distribution and persistence of probiotic Streptococcus salivarius K12 in the human oral cavity as determined by real-time quantitative polymerase chain reaction. Oral Microbiol Immunol 2007;22(2):126-30.

Haukioja A. Probiotics and oral health. Eur J Dent 2010;4(3):348-55.

Fauzi AA, Shafiei Z, Baharin B, Mohd N. Isolation of Lactobacillus from periodontally healthy subjects and its antimicrobial activity

against periodontal pathogens. Sains Malaysiana 2013;42(1):19-24.

Burton JP, Wescombe PA, Macklaim JM, Chai MH, Macdonald K, Hale JD, et al. Persistence of the oral probiotic Streptococcus salivarius

M18 is dose dependent and megaplasmid transfer can augment their bacteriocin production and adhesion characteristics. PLoS One

;8(6):e65991.

Kementerian Kesehatan Republik Indonesia. Profil Kesehatan Indonesia Tahun 2011. Jakarta: Kementerian Kesehatan; 2012.

Wescombe PA, Hale JD, Heng NC, Tagg JR. Developing oral probiotics from Streptococcus salivarius. Future Microbiol 2012;7(12):1355-71.

Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients 2010;2(3):290-8.

Roger P, Delettre J, Bouix M, Béal C. Characterization of Streptococcus salivarius growth and maintenance in artificial saliva. J Appl Microbiol 2011;111(3):631-41.

McAuliffe O, Ross RP, Hill C. Lantibiotics: Structure, biosynthesis and mode of action. FEMS Microbiol Rev 2001;25(3):285-308.

Alakomi HL, Skyttä E, Saarela M, Mattila-Sandholm T, Latva- Kala K, Helander IM. Lactic acid permeabilizes Gram-negative

bacteria by disrupting the outer membrane. Appl Environ Microbiol 2000;66(5):2001-5.

Parada JL, Caron CR, Medeiros AB, Soccol CR. Bacteriocins from lactic acid Bacteria: Purification, properties and use as biopreservatives.

Braz Arch Biol Technol 2007;50:521-42.

Burton JP, Drummond BK, Chilcott CN, Tagg JR, Thomson WM, Hale JD, et al. Influence of the probiotic Streptococcus salivarius strain

M18 on indices of dental health in children: A randomized doubleblind, placebo-controlled trial. J Med Microbiol 2013;62:875-84.

Gauthier M, Brochu D, Eltis LD, Thomas S, Vadeboncoeur C. Replacement of isoleucine-47 by threonine in the HPr protein of

Streptococcus salivarius abrogates the preferential metabolism of glucose and fructose over lactose ana melibose but does not

prevent the phosphorylation of Hpr on serine-46. Mol Microbiol 1997;25(4):695-705.

Weerkamp AH, McBride BC. Adherence of Streptococcus salivarius HB and HB-7 to oral surfaces and saliva-coated hydroxyapatite. Infect

Immun 1980;30(1):150-8.

Brötz H, Sahl HG. New insights into the mechanism of action of lantibiotics-diverse biological effects by binding to the same molecular

target. J Antimicrob Chemother 2000;46(1):1-6.

Barbour A, Philip K, Muniandy S. Enhanced production, purification, characterizatio n and mechanism of action of salivaricin 9

lantibiotic produced by Streptococcus salivarius NU10. PLoS One 2013;8(10):e77751.

Published

01-10-2017

How to Cite

Vaniabella, R., H. Joenoes, and B. M. Bachtiar. “ANALYSIS ON THE INHIBITION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS BY STREPTOCOCCUS SALIVARIUS ISOLATED FROM SALIVA AND TONGUE DORSUM OF ADULTS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 17, Oct. 2017, pp. 6-10, doi:10.22159/ajpcr.2017.v10s5.23081.

Issue

Section

Original Article(s)