ANTIBACTERIAL EFFECT OF JAVA TURMERIC ETHANOL EXTRACT AGAINST DUAL-SPECIES STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS BIOFILM (IN VITRO)

Ajrina Busri; Ria Puspitawati; Sri Utami

doi:10.22159/ajpcr.2017.v10s5.23096

Authors

Ajrina Busri Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Ria Puspitawati Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Sri Utami Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23096

Keywords:

Dual-species Streptococcus, Java turmeric ethanol extract, Streptococcus mutans, Streptococcus sanguinis

Abstract

Objectives: The minimal bactericidal concentration (MBC) of Java turmeric (Curcuma xanthorrhiza Roxb.) ethanol extract is 25% against Streptococcus mutans and 15% against Streptococcus sanguinis as single species. This study aimed to examine the antibacterial effect of Java turmeric ethanol extract against S. mutans and S. sanguinis as dual-species. S. mutans and S. sanguinis compete against each other to obtain nutrients.

Methods: The antibacterial effect of Java turmeric ethanol extract against dual-species Streptococcus in vitro was analyzed by measuring the growth of bacteria after exposure to the extract by counting colony formation and quantifying bacterial cell numbers using real-time polymerase chain reaction.

Result: The MBC of Java turmeric ethanol extract against dual-species Streptococcus is 10%. S. sanguinis is more sensitive to the extract than S. mutans.

Conclusions: The antibacterial effect of Java turmeric ethanol extract on S. mutans and S. sanguinis as single species differs from the effect on the bacteria as dual-species of Streptococcus.

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