THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT'S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION

Sri Angky Soekanto; GADIA CANAPARIMITA GHRENA  DUHITA; Endang Winiati Bachtiar; Muhamad Sahlan

doi:10.22159/ajpcr.2017.v10s5.23125

Authors

Sri Angky Soekanto Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
GADIA CANAPARIMITA GHRENA DUHITA Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Endang Winiati Bachtiar Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Muhamad Sahlan Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23125

Keywords:

Caries, Saliva, Casein phosphopeptide-amorphous calcium phosphate, Propolis wax, Biofilm

Abstract

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Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.

Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.

Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).

Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

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