EFFICACY OF A STANDARDIZED ETHANOL EXTRACT OF ROSELLE CALYX IN THE TREATMENT OF ORAL MUCOSA ULCERATION (IN VIVO)
DOI:
https://doi.org/10.22159/ajpcr.2017.v10s5.23127Keywords:
Standardized ethanol extract of roselle calyx, Ulcer, HealingAbstract
Â
Objectives: To analyze the effectiveness of the topical application of a standardized ethanol extract of roselle calyx in the treatment of oral mucous ulceration.
Methods: Twelve Sprague Dawley rats were divided into two groups, with oral mucous ulcer being induced in all the rats. The ulcer diameter, reddish color, and the swollen membrane around the ulcer, as well as the inflammation score of the ulcer, were recorded for all animals. The negative control group was treated with aquades, while the positive control group was treated with 0.1% triamcinolone acetonide. The treatment group received the topical application of 7.5% and 15% standardized ethanol extract of roselle calyx twice a day for 3 or 7 days. On the 4th day, six rats were sacrificed, while the remaining six rats continued to receive treatment as before. On the 8th day, all the rats were sacrificed. Specimens were fixed, and histology slides were made. Further, microscopic slides were observed and scored.
Result: Both the 7.5% and 15% standardized ethanol extracts of roselle calyx were found to reduce the ulcer diameter and inflammation score. No swelling or redness was observed.
Conclusions: The application of 7.5% standardized ethanol extract of roselle calyx for 7 days is equally as effective as the application of 0.1% triamcinolone acetonides, except in terms of the decrease in the inflammation score.
Downloads
References
Notoadmodjo S. Kesehatan Masyarakat: Ilmu dan Seni. Jakarta: Rineka
Cipta; 2007. p. 330-9.
Indonesian Regulation No. 23; 1992.
Indonesian Ministry of Health Regulation No. 1076/MENKES/SK/VII;
Suwandi T. Pengembangan Potensi Antibakteri Kelopak Bunga
Hibiscus sabdariffa Linn (Rosela) Terhadap Streptococcus sanguinis
Penginduksi Gingivitis Menuju Obat Herbal Terstandar (Disertation).
Jakarta: Universitas Indonesia; 2012.
Abdulkadir W, Polontao FR. An inflammatory effect test of rosela
flowers (Hibiscus sabdariffa Lim) on male rats (Rattus norvegicus).
J Health Sport 2011;3(2):349-62.
Indonesian Food and Drugs Administration. Rosela (Hibiscus
Sabdariffa L.). Jakarta: Indonesian FDA; 2010. p. 1-18.
Ali MK, Ashraf A, Biswas NN, Karmakar UK, Afroz S. Antinociceptive,
anti-inflammatory and antidiarrheal activities of ethanolic calyx extract
of Hibiscus sabdariffa Linn. (Malvaceae) in mice. Zhong Xi Yi Jie He
Xue Bao 2011;9(4):626-31.
Thakur R, Jain N, Pathak R, Sandhu SS. Practices in wound
healing studies of plants. Evid Based Complement Alternat Med
;2011:438056.
Kao ES, Hsu JD, Wang CJ. Polyphenol extracted from Hibiscus
Sabdariffa L. inhibited lipopolysaccharide-induced inflammation by
improving antioxidative conditions and regulating cyclooxygenase-2
expression. Biotechnology 2009;73(2):385-90.
Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K,
van Leeuwen PA. Flavonoids: A review of probable mechanisms of
action and potential applications. Am J Clin Nutr 2001;74(4):418-25.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
