DEVELOPMENT OF ULTRAVIOLET-SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF AMOXAPINE IN PHARMACEUTICAL DOSAGE FORM

Shweta Rangari; Shraddha Patil; Rutuja Mahajan; Abrar Ahmed; Nishikant A Raut

doi:10.22159/ajpcr.2018.v11i3.23154

Authors

Shweta Rangari Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur â€“ 440 033, Maharashtra, India.
Shraddha Patil Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur â€“ 440 033, Maharashtra, India.
Rutuja Mahajan Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur â€“ 440 033, Maharashtra, India.
Abrar Ahmed Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur â€“ 440 033, Maharashtra, India.
Nishikant A Raut Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur â€“ 440 033, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i3.23154

Keywords:

Amoxapine, Ultraviolet-spectrophotometry, Method development, Validation

Abstract

Â Objective: Knowing the exact amount of active pharmaceutical ingredient (API) in pharmaceutical dosage form is of utmost importance to meet regulatory requirements and to ensure patient safety. Spectrophotometric analysis provides a simple, efficient, and economic approach for estimation of API in the pharmaceutical dosage form. In the present work, we have developed simple, sensitive, and highly economic ultraviolet (UV) spectrophotometric method for the estimation of amoxapine in a pharmaceutical formulation.

Methods: Amoxapine shows maximum absorbance of light at wavelength 297 nm in water. The linearity study revealed that it obeys Beer-Lambert's law over the range of 2â€“20 Î¼g/mL. Absorptivity value of amoxapine was found to be 206.6Â±1.341.

Result: The tablet formulation was successfully analyzed by developed UV spectrophotometric method. The developed method was validated as per International Conference on Harmonization guidelines with respect to accuracy, precision, specificity robustness . The limit of detection and limit of quantitation was found to be 19.8 and 60.50 ng/mL, respectively.

Conclusion: The developed method is simple, precise, accurate, and cost-effective and can be used for routine analysis of amoxapine.

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References

Lotfy HM, Saleh SS. Recent development in ultraviolet spectrophotometry through the last decade (2006-2016): A review. Int J Pharm Pharm Sci 2016;8:40-56.

Zadbuke N, Shahi S, Jadhav A, Borde S. Development and validation UV-visible spectrophotoscopic method for estimation of carbazepine in bulk and tablet dosage form. Int J Pharm Pharm Sci 2016;8:234-8.

Jue S, Dawson G, Brogden, R. Amoxapine: A review of its pharmacology and efficacy in depressant state. Drug Eval 1982;24:1-23.

Apiquian R, Ulloa R, Fresan A, Loyzaga C, Nicolini H, Kapur S. Amoxapine shows atypical antipsychotic effect in patients with schizophrenia: Results from a prospective open-label study. Schizophr Res 2002;59:35-9.

Tassel J, Hassan F. Liquid chromatographic determination of amoxapine and 8-hydroamoxapine in human serum. Clin Chem 1982;28:2154-62.

Selinger K, Lebel G, Hill H, Anslow J. A high performance liquid chromatographic method for the analysis of amoxapine in human plasma. J Pharm Biomed Anal 1989;8:1001-7.

Vijaykrishna AC, Samir SP, Ahmed M, Shetty AS, Kuppast IJ, Anilkumar SM, et al. RP-HPLC method development and validation for estimation of amoxapine in tablet dosage form. World J Pharm Pharm Sci 2015;4:1113-9.

Gupta M, Jain A, Verna K. Determination of Amoxapine and nortriptylline liquid-liquid micro extraction and high performance liquid chromatography. J Sep Sci 2010;33:3774-80.

Steven M, Jain A, Verna K. Determination of antidepressant, maprotiline and amoxapine and their metabolite in plasma by liquid chromatography. Clin Chem 1983;29:314-8.

Zimmer JS, Needham SR, Christianson CD, Piekarski CM, Sheaff CN, Huie K, et al. Validation of HPLC-MS/MS methods for analysis of loxapine, amoxapine,7-OH-loxapine, 8-OH-loxapine and N-oxide in human plasma. Bioanalysis 2010;2:1989-2000.

Karasakal A, Ulu S. Development and validation of a sensitive spectrfluoririmetric method for the determination of amoxapine in human plasma and urine. J Biol Chem Lumin 2013;29:284-7.

Korrapati U, Chintala R. New stability indicating RP-HPLC and spectrophotometric methods for the determination of amoxapine in tablet dosage form. Anal Chem 2016;16:147-56.

Patel S, Vijaykrishna C, Shetty S, Ahmad M. Development and validation of first order derivative spectrophotometric method for estimation of amoxapine in bulk and tablet dosage form. Int J Univ Pharm Biol Sci 2015;4:29-35.

International Conference of Harmonization: Q2 (R1) Validation of Analytical Procedure: Methodology: Geneva; 1996.