ISOLATION AND CHARACTERIZATION OF PHYTOSTEROLS FROM CORDIA MACLEODII (HOOK F. AND THOMSON) BARK BY CHROMATOGRAPHIC AND SPECTROSCOPIC METHOD
Abstract
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 Aims and objectives: The study focuses on isolation and determination of the chemical constituents of the Cordia macleodii bark, used as medicinal plant in folklore system. The principal theme of the study is to develop applied chromatographic techniques for the separation, isolation and detection of the compounds.
Methods: A petroleum extract of bark were analyzed by GC/MS, IR, and UV. The structures were elucidated on the basis GC-MS library of reported data.
Result: Three known compounds Stigmasterol, Cholest-5-EN -3OL (3 Beta)-Carbonyl chlorinated, Camphesterol were determined from Cordia macleodii bark. These compounds were isolated from this plant for the first time.
Conclusion: From the present study, it is concluded that chromatographic and spectroscopy has potential as rapid and simple tools in the isolation and analysis of various compounds from Cordia macleodii bark.
Keywords: Gas chromatography-mass spectrometry, Infrared, Chromatography, Spectroscopy, Phytosterols, Cordia macleodii
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