SCREENING ANTIBACTERIAL EFFECTS OF VIETNAMESE PLANT EXTRACTS AGAINST PATHOGENS CAUSED ACUTE HEPATOPANCREATIC NECROSIS DISEASE IN SHRIMPS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i5.23618Keywords:
Medicinal plant, Rhodomyrtus tomentosa, Antibacterial effect, Acute hepatopancreatic necrosis disease, ShrimpAbstract
Objectives: The objectives are aimed to investigate the antibacterial properties of five Vietnamese medicinal plants against acute hepatopancreatic necrosis disease (AHPND)-caused bacterial pathogens, to verify their potentials to apply as a new treatment therapy.
Methods: Extracts from plants, such as Psidium guajava leaf, Piper betle L. leaf, Phyllanthus amarus leaf, Rhodomyrtus tomentosa seed, and Allium sativum bulb, were tested against three AHPND-caused bacteria. Agar infusion and broth dilution methods were employed to evaluate extract in vitro antibacterial effects, while experiments with cultured whiteleg shrimps were applied to access their safety when applied in vivo. High-performance liquid chromatography (HPLC) analysis was applied to identify components in the extracts.
Results: P. amanus and R. tomentosa extracts exerted the strongest inhibition on tested bacteria. Other extracts, including P. betel and P. guajava, were less effective, while A. sativum showed no effects against bacteria. In safety assessment experiments, we observed that only crude extracts of R. tomentosa and A. satium were safe, while others significantly reduced their survival rates. HPLC showed that extracts of high antibacterial properties had rich phenol constituents. In addition, the phenolic profile of R. tomentosa showed the presence of piceatannol.
Conclusion: Considering both of antibacterial effects and safety properties altogether, we concluded that among the five examined plant materials of this study, R. tomentosa had the highest potential to apply in AHPND treatment, as only this plant showed the high effects on pathogenic bacteria while were still safe for host aquatic shrimps.
Downloads
References
Tran L, Nunan L, Redman RM, Mohney LL, Pantoja CR, Fitzsimmons K, et al. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Dis Aquat Organ 2013;105:45-55.
Flegel TW. Historic emergence, impact and current status of shrimp pathogens in Asia. Invertebr Pathol 2012;110:166-73.
Thitamadee S, Prachumwat A, Srisala J, Jaroenlak P, Salachan PV, Sritunyalucksana K, et al. Review of current disease threats for cultivated penaeid shrimp in Asia. Aquaculture 2016;452:69-87.
Kondo H, Van PT, Dang LT, Hirono I. Draft genome sequence of Non-vibrio parahaemolyticus acute hepatopancreatic necrosis disease strain KC13.17.5, isolated from diseased shrimp in Vietnam. Genome Announc 2015;3:e00978-15.
Zorriehzahra MJ, Banaederakhshan R. Early mortality syndrome (EMS) as new emerging threat in shrimp industry. Adv Anim Vet Sci 2015;3:64-72.
Dang LT, Nguyen KV, Pham VT. Non-vibrio parahaemolyticus gay benh hoai tu gan tuy cap (AHPND) tren tom nuoi. Vietnam J Agric Sci 2016;14:690-8.
Immanuel G, Vincybai VC, Sivaram V, Palavesam A, Marian MP. Effect of butanolic extracts from terrestrial herbs and seaweeds on the survival, growth and pathogen (Vibrio parahaemolyticus) load on shrimp Penaeus indicus juveniles. Aquaculture 2005;236:53-65.
Li J, Tan B, Mai K, Ai Q, Zhang W, Xu W, et al. Comparative study between probiotic bacterium Arthrobacter XE-7 and chloramphenicol on protection of Penaeus chinensis post-larvae from pathogenic vibrios. Aquaculture 2006;253:140-7.
Ngo VH. The use of medicinal plants as immunostimulants in aquaculture: A review. Aquaculture 2015;446:88-96.
Kirubakaran CJ, Alexander CP, Michael RD. Enhancement of non-specific immune responses and disease resistance on oral administration of Nyctanthes arbortristis seed extract in Oreochromis mossambicus (Peters). Aquac Res 2010;41:1630-9.
Han JE, Mohney LL, Tang KF, Pantoja CR, Lightner DV. Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquac Rep 2015;2:17-21.
Do, T.L. Nhung cay thuoc va vi thuoc thuong dung tai Vietnam. [Book in Vietnamese]. 13rd ed. Hanoi, Vietnam: Vietnam Medical Publishing House; 2005. p. 97-435. (a): Cay oi. Psidium guajava. In: Do, T.L. (Eds.), pp. 431-432. (b): Cho de rang cua. Phyllanthus amarus. In: Do, T.L. (Eds.), Hanoi, Vietnam: Vietnam Medical Publishing House; 2005. p. 97-98. (c): Trau khong. Piper betel L. In: Do, T.L. (Eds.), Hanoi, Vietnam: Vietnam Medical Publishing House; 2005. p. 118-119. (d): Sim. Rhodomyrtus tometosa Wight. In: Do, T.L. (Eds.), Hanoi, Vietnam: Vietnam Medical Publishing House; 2005. p. 434-435. (e): Toi. Allium sativum L. In: Do, T.L. (Eds.), Hanoi, Vietnam: Vietnam Medical Publishing House; 2005. p. 181-182.
Le, V.T. and Nguyen, G.C. Selected Medicinal plants in Vietnam. 1st ed., Vol. I, II. Hanoi, Vietnam: Vietnam National Institute of Materia Medica Publications, Science and Technology Publishing House; 1999. (a): Piper betle L. - Piperaceae. In: Le, V.T. and Nguyen, G.C. (Eds.), 1st ed., Vol. II. Hanoi, Vietnam: Vietnam National Institute of Materia Medica Publications. Science and Technology Publishing House; 1999. p. 179-182. (b): Pluchea indica (L.) Less. Asteraceae. In: Le, V.T. and Nguyen, G.C. (Eds.), 1st ed., Vol. II. Hanoi, Vietnam: Vietnam National Institute of Materia Medica Publications. Science and Technology Publishing House; 1999. p. 189-192. (c): Rhodomyrtus tomentosa (Ait.) Hassk. – Myrtaceae. In: Le, V.T. and Nguyen, G.C. (Eds.), 1st ed., Vol. II. Hanoi, Vietnam: Vietnam National Institute of Materia Medica Publications. Science and Technology Publishing House; 1999. p. 241-243.
Bui TT, editor. Tac Dung Khang Khuan Cua Thao Duoc: Phyto-antibacterial effects. [Document in Vietnamese]. Phuong Phap Nghien Cuu cay Thuoc-Tai Lieu Tham Khao Cho Nghien Cuu Sinh va hoc Vien cao hoc lam de tai Nghien cuu ve cay Thuoc (luu hanh noi bo trong phong thi nghiem). Hanoi, Vietnam: Department of Internal Medicine and Pharmacology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture; 2012. p. 18-9.
Dang TL, editor. Ket qua nghien cuu tac dung cua mot so cay thuoc voi vi khuan gay benh thuy san. [Document in Vietnamese]. Phuong Phap Nghien Cuu Thao Duoc Voi Benh Thuy san (Luu Hanh Noi Bo Trong Phong Thi Nghiem). Tu Son, Bac Ninh, Vietnam: Center for Environment and Disease Monitoring in Aquaculture, Research Institute for Aquaculture No. 1; 2012. p. 8-21.
Bui TT, Nguyen TH. Tap Hop Cac Ket Qua Nghien Cuu Ve Tac Dung Cua Thuc Vat Voi Vi Khuan Thu y [Document in Vietnamese]. In: Nguyen TH, editros. Tap Hop Cac Ket Qua Nghien Cuu Khoa Hoc Ve Cay Thuoc Cua Sinh Vien Dai Hoc (Luu Hanh Noi Bo Trong Phong Thi Nghiem Duoc Ly Thu Y). Gia Lam, Hanoi, Viet Nam: Laboratory of Veterinary pharmacology, Vietnam National University of Agriculture; 2010. p. 38-40.
Phan VT, Dang LT, Nguyen KV, Bui TN, Pham VT, Pham YT, et al. Nghien cuu xac dinh nguyen nhan gay benh hoai tu gan tuy cap tren tom tai phia Bac. Bao cao tong ket de tai cap Bo. (Studies on the cause of AHPND in shrimp cultured in Northern Vietnam. Final National project report) (In Vietnamese language). 2014.
Sirikharin R, Taengchaiyaphum S, Sritunyalucksana K, Thitamadee S, Flegel TW, Mavichak R, et al. New and Improved PCR Method for Detection of AHPND Bacteria. Available from: http://www.enaca.org/modules/news/article.php?article_id=230&title= new-pcr- detection-method-for-ahpnd 2014. [Last cited on 2014 Jun 18].
Lai TN, Andre CM, Chirinos R, Nguyen TB, Larondelle Y, Rogez H. Optimisation of extraction of piceatannol from Rhodomyrtus tomentosa seeds using response surface methodology. Sep Purif Technol 2014;134:139-46.
Bauer AW, Kirby WM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1966;45:493-96.
Lai TN, Herent MF, Quetin-Leclercq J, Nguyen TB, Rogez H, Larondelle Y, et al. Piceatannol, a potent bioactive stilbene, as major phenolic component in Rhodomyrtus tomentosa. Food Chem 2013;138:1421-30.
Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover, W. Phenolic compounds and their role in oxidative processes in fruits. Food Chem 1999;66:401-36.
Asha S, Anitha S, Anantharajan R. Antibacterial activity of herbal plant extracts towards the fish pathogens. Internet J Microbiol 2007;4:1-5.
Direkbusarakom S. Application of medicinal herbs to aquaculture in Asia. Walailak J Sci Tech 2004;1:7-14.
Pirbalouti G, Hamedi B, Malek Poor F, Rahimi E, Nasri Nejhad R. Inhibitory activity of Iranian endemic medicinal plants against Vibrio parahaemolyticus and Vibrio harveyi. J Med Plants Res 2011;5:7049-53.
Sankar G, Ramamoorthy K, Sakkaravarthi K, Elavarsi A. Antibacterial activity of herbal extract on pathogens isolated from the swollen hind gut of P. Monodon (Fabricus). Pharm Sin 2010;1:17-22.
Sivaram V, Babu MM, Immanuel G, Murugadass S, Citarasu T, Marian MP. Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio harveyi infections. Aquaculture 2004;237:9-20.
Velmurugan S, Punitha SM, Babu MM, Selvaraj T, Citarasu T. Indian herbal medications to replace antibiotics for shrimp Penaeus monodon post larvae. J Appl Aquacult 2010;22:230-9.
Velmurugan S, Viji VT, Babu MM, Punitha MJ, Citarasu T. Antimicrobial effect of Calotropis procera active principles against aquatic microbial pathogens isolated from shrimp and fishes. Asian Pacific J Trop Biomed 2012;2:S812-7.
Kua BC, Ahmad IA, Siti-Zahrah A, Nik HN, Fadzilah Y, Irence J. Effectiveness of Betel Leave (Piper betle) and Lemongrass (Cympopogon citratus) Extracts on Challenged Whiteleg shrimp, Litopenaeus vannamei with Vibrio parahaemolyticus that Caused AHPND. Ho Chi Minh City, Vietnam: Poster Presented at 9th Symposium on Diseases in Asian Aquaculture; 2014. p. 91.
Dhandapani R, Lakshmi D, Balakrishnan V, Jayakumar S, Kumar A. Preliminary phytochemical investigation and antibacterial activity of Phyllanthus amarus Schum & Thorn. Anc Sci Life 2007;27:1-5.
Limsuwan S, Kayser O, Voravuthikunchai SP. Antibacterial activity of Rhodomyrtus tomentosa (Aiton) hassk. Leaf extract against clinical isolates of Streptococcus pyogenes. Evid Based Complement Alternat Med 2012;2012:697183.
Morais-Braga MF, Carneiro JN, Machado AJ, Dos Santos AT, Sales DL, Lima LF, et al. Psidium guajava L., from ethnobiology to scientific evaluation: Elucidating bioactivity against pathogenic microorganisms. J Ethnopharmacol 2016;194:1140-52.
Valle DL Jr, Cabrera EC, Puzon JJ, Rivera WL. Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of Philippine Piper betle L. On clinical isolates of gram positive and gram negative bacteria with transferable multiple drug resistance. PLoS One 2016;11:e0146349.
Deshpande SN, Kadam DG. GCMS analysis and antibacterial activity of Piper betle (Linn) leaves against Streptococcus mutans. Asian J Pharm Clin Res 2013;6:99-101.
Hussain RM, Ho FK, Mustakim M, Suhaimi H. Upregulation of TRXB expression by Piper betel Linn ethanolic extract suggests induction of oxidative stress which potentially contributes to killing of Staphylococcus aureus in vitro. Int J Pharm Pharm Sci 2015;7:154-9.
Jayalakshmia B, Raveeshab KA, Muralic M, Amruthesh KN. Phytochemical, antibacterial and antioxidant studies on leaf extracts of Piper betle L. Int J Pharm Pharm Sci 2015;7:23-9.
Buzzini P, Arapitsas P, Goretti M, Branda E, Turchetti B, Pinelli P, et al. Antimicrobial and antiviral activity of hydrolysable tannins. Mini Rev Med Chem 2008;8:1179-87.
Paulo L, Ferreira S, Gallardo E, Queiroz JA, Domingues F. Antimicrobial activity and effects of resveratrol on human pathogenic bacteria. World J Microbiol Biotechnol 2010;26:1533-8.
Piotrowska H, Kucinska M, Murias M. Biological activity of piceatannol: Leaving the shadow of resveratrol. Mutat Res 2012;750:60-82.
Plumed-Ferrer C, Väkeväinen K, Komulainen H, Rautiainen M, Smeds A, Raitanen JE, et al. The antimicrobial effects of wood-associated polyphenols on food pathogens and spoilage organisms. Int J Food Microbiol 2013;164:99-107.
Schrader KK. Plant natural compounds with antibacterial activity towards common pathogens of pond-cultured channel catfish (Ictalurus punctatus). Toxins (Basel) 2010;2:1676-89.
Hamid HA, Mutazah SS, Yusoff MM. Rhodomyrtus tomentosa: A phytochemical and pharmacological review. Asian J Pharm Clin Res 2017;10:10-6.
Agro Forestry Tree Database. Tree Species Reference and Selection Guide: Rhodomyrtustomentosa. Available from: http://www.worldagroforestrycentre.org/sea/Products/AFDbases/af/asp/SpeciesInfo.asp?SpID=18093. [Last accessed on 2011 Sep 24].
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
