GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON ETHANOLIC EXTRACT OF DRIED LEAVES OF CATHARANTHUS ROSEUS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i6.23704Keywords:
Catharanthus roseus, Gas chromatography, Mass spectroscopy, Phytol, 3-Epivindolinine, Oleic acidAbstract
Objective: Phytochemical screening of the ethanolic extract of Catharanthus roseus disclosed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, flavonoids, and proteins. In the present research work, we have identified and confirmed the structures of the constituents present by means of a hyphenated technique of gas chromatography-mass spectrometry (GC-MS) from the extract.
Method: Shade-dried leaves of C. roseus were powdered and extracted by means of Soxhlet extraction using ethanol as a solvent. Crude extract obtained was utilized for GC-MS.
Results: A total of 15 components were identified, some of which were n-hexadecanoic acid, ethyl ester, Vitamin E, 9,12,15-octadecatrienoic acid,2- [(trimethylsilyl)oxy]-1-[{(trimethylsilyl)oxy}methyl]ethyl ester, phytol, 3-epivindolinine, and oleic acid.
Conclusion: GC-MS studies helped us to assess the phytochemical constituents based on retention time, molecular formula, molecular weight and the corroboration of MS libraries. In future, C. roseus leaves may be subjected to diverse types of extraction methodologies, and a number of various elucidated phytoconstituents can be studied for their ethnopharmacological significance and applicability citing literature.
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References
Harborne JB. Phytochemical Methods. 3rd ed. New Delhi: Springer Publication House; 2005. p. 1-36.
Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J 2006;8:E239-53.
Butler MS. The role of natural product chemistry in drug discovery. J Nat Prod 2004;67:2141-53.
Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. J Pharm Phytochem 2013;1:168-82.
Kabesh K, Senthilkumar P, Ragunathan R, Kumar RR. Phytochemical analysis of Catharanthus roseus plant extract and its antimicrobial activity. Int J Pure Appl Biosci 2015;3:162-72.
Hossain S, Hossain M, Haque Z, Moyen Uddin PK. Phytochemical Screening of Catharanthus Roseus and Ficus Racemosa Leaves Extracts: A statistical inference. Int J Bioassays 2015;4:3606-10.
Aja PM, Okorie UC, Ozougwu VE, Onya-Mmag EA, Agu KA, Nweke OL. Phytochemical and gas chromatography-mass spectrometric (GC-MS) analyses of Whitfieldia lateritia Leaf. Afr J Basic Appl Sci 2016;8:41-54.
Das S, Sharangi AB. Madagascar periwinkle (Catharanthus roseus L.): Diverse medicinal and therapeutic benefits to humankind. J Pharm Phytochem 2017;6:1695-701.
Lwashina T. The structure and distribution of the flavonoids in plants. J Plant Res 2000;113:287-99.
Liu J, Liu Y, Pan Y, Zu YG, Tang ZH. Determination of alkaloids in Catharanthus roseus and Vinca minor by high-performance liquid chromatography–tandem mass spectrometry. Anal Lett 2016;49:1143-53.
Gopal KR, Bharathi VD, Sreemathi M, Krishnamoorthy C. Effect of different methods of drying on quality parameters in periwinkle (Catharanthus Roseus L.). Int J Sci Nat 2016;7:594-7.
Rency RC, Vasantha K, Maruthasalam A. Identification of bioactive compounds from ethanolic leaf extracts of Premna serratifolia L. using GC-MS. Biosci Disc 2015;6:96-101.
Karuppasamy R, Veerabahu RM. GC-MS analysis of bioactive components of Myxopyrum serratulum A.W. Hill (Oleaceae). Int J Pharm Sci Rev Res 2016;38:30-5.
Chaskar PK, Tank SH, Doshi GM. Gas chromatography-mass spectroscopy studies on Cestrum nocturnum macerated methanolic extract. Asian J Pharm Clin Res 2017;10:259-63.
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