EVALUATION OF GENOTOXIC EFFECTS OF ALCOHOLIC EXTRACT OF KALANCHOE PINNATA USING SINGLE CELL GEL ELECTROPHORESIS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i5.23964Keywords:
COMET, Ethanolic extract, Kalanchoe pinnata, Lymphocytes, MutagenicAbstract
 Objective: The objective of the study was to evaluate the genotoxic and mutagenic potentials of Kalanchoe pinnata alcoholic leaves extract.
Methods: Single cell gel electrophoresis (COMET assay) was adopted to investigate genotoxicity potential of standardized ethanolic leaves extract at the concentration of 10, 50, and 100 μg/ml, respectively, using human lymphocytes.
Results: K. pinnata leaves extract did not completely damage DNA in human lymphocytes but low, medium, and high-level damage was found in with positive control. The DNA damage level decreases in low, medium, and high (12±1, 6±1, and 1±1) without positive control at the concentration of 100 μg/ml.
Conclusion: It was concluded that the parent material did not induce mutagenic and genotoxic potentials at the concentrations 10, 50, and 100 μg/ml but its metabolites are inducing genotoxicity in various models.
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