PROTECTIVE ROLE OF AERVA MONSONIAE AND SELENIUM ON CADMIUM- INDUCED OXIDATIVE LIVER DAMAGE IN RATS

Divya Jyothi Palati; Swaroopa Rani Vanapatla

doi:10.22159/ajpcr.2018.v11i6.24112

Authors

Divya Jyothi Palati Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India.
Swaroopa Rani Vanapatla Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.24112

Keywords:

Cadmium, Selenium, Aerva monsoniae, Oxidative damage, enzymatic antioxidants

Abstract

Objective: The present study was aimed to investigate the protective effect of methanolic extract of the whole plant of Aerva monsoniae (MEAM) and selenium on cadmium (Cd)-induced oxidative liver damage in experimental rats.

Methods: In the present study, albino Wistar rats were treated with Cd (5 mg/kg), selenium (1 mg/kg), and MEAM (250 and 500 mg/kg) for 21days. After 21 days of the treatment, the rats were sacrificed, and blood was collected for estimation of biochemical parameters and liver was used for histopathological studies.

Results: Oral administration of Cd significantly elevated the levels of hepatic markers such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, Î³-glutamyl transferase, cholesterol, total bilirubin, direct bilirubin, and decreased levels of total proteins and albumin. We also observed that elevated oxidative stress markers such as malondialdehyde reduced the enzymatic antioxidants such as superoxide dismutase, catalase, and non-enzymatic antioxidants such as reduced glutathione in the liver. Co-administration of MEAM and selenium in Cd-intoxicated rats, the altered biochemical parameters, and pathological changes were recovered significantly (p>0.01) than the individual effects of MEAM and selenium.

Conclusion: From the above findings, it was concluded that combination of MEAM and selenium exhibited remarkable protective effects against Cd-induced oxidative liver damage in rats.

Downloads

Download data is not yet available.

Author Biography

Divya Jyothi Palati, Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India.

Faculty of Pharmaceutical sciences

References

OgnjanoviÄ‡ BI, MarkoviÄ‡ SD, PavloviÄ‡ SZ, ZikiÄ‡ RV, Stajn AS, SaiciÄ‡ ZS, et al. Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium. Physiol Res 2008;57:403-11.

Waisberg M, Joseph P, Hale B, Beyersmann D. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 2003;192:95-117.

Bais S, Chandewar A. Toxicological standardisation of marketed Ashwagandha formulations by atomic absorption spectroscopy. Asian J Pharm Clin Res 2013;6:45-8.

Bandyopadhyay D, Ghosh D, Chattopadhayay A, Mitra E. Curry leaves as alternative medicine in heavy metals induced occupational health hazards. Int J Pharm Pharm Sci 2016;8:8-20.

Bagchi D, Bagchi M, Hassoun EA, Stohs SJ. Cadmium induced excretion of urinary lipid metabolite, DNA damage, glutathione depletion and hepatic lipid peroxidation in Sprague-Dawley rats. Biol Trace Elem Res 1996;52:143-54.

Stohs SJ, Bagchi D, Hassoun E, Bagchi MM. Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ Pathol Toxicol Oncol 2000;19:201-13.

Rayman MP. The importance of selenium to human health. Lancet 2000;356:233-41.

Newairy AA, EI-Sharky AS, Badreldeen MM, Eweda SM, Sheweita SA. The hepatoprotective effects of Selenium against Cadmium toxicity in rats. Toxicology 2007;242:23-30.

Sandhya S, Sai KP, Vinod KR, Banji D, Kumar K, Rajeshwar T. In ova angiogenesis analgesic and anti-inflammatory potency of Aerva monsoniae (Amaranthaceae). Asian Pac J Trop Dis 2012;2:385-9.

Swaroopa Rani V, Praneetha P, Krishna Mohan G, Ravi Kumar B. Antihyperglycemic activity of Aerva monsoniae. Int J Phytomed 2014;6:395-404.

Chiluka R, Gamble AB, Lucas NT, Hawkins BC, Bhumkar A, Achanta PS, et al. Flavone C-glycosides from Trichuriella monsoniae (L.f.) bennet. Nat Prod Res 2016;30:2235-7.

Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:315-8.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biol 1984;21:130-2.

Yi-Chun L, Kuei-Mei C, Huang HY, Pei-Yu C, Jin-Ming H, Hsuesh- Hui L, et al. Hepatoprotrctive activity of Chhit-Chan-Than extract powder against carbon tetrachloride induced liver injury in rats. J Food Drug Anal 2014;22:220-9.

Williamson EM, Okpako DT, Evans FJ. Selection, Preparation and Pharmacological Evaluation of Plant Material. England: John Wiley; 1996. p. 1.

Burtis CA, Ashwood ER. Text Book of Clinical Chemistry. Philadelphia, Pennsylvania: W.B. Saunders Company; 1986.

Meister S. Commentary on the antioxidant effect of ascorbic acid and glutathione. Biochem Pharmacol 1992;44:1905-15.

Rosalki SB, Rao D, Lchman D, Prentice M. Determination of serum gamma-glutamyl transpeptitase activity and its clinical applications. Ann Clin Biochem 1970;7:143-7.

Rogalska J, Brzoska MM, Roszczenko A, Moniuszko-Jakoniuk J. Enhanced zinc consumption prevents cadmium-induced alterations in lipid metabolism in male rats. Chem Biol Interact 2009;177:142-52.

Ige SF, Akhigbe RE, Edeogho O, Ajao FO, Owolabi OQ, Oyekunle OS, et al. Hepatoprotective activities of Allium cepa in cadmium-treated rats. Int J Pharm Pharm Sci 2011;3:60-3.

Shaikh ZA, Vu TT, Zaman K. Oxidative stress as a mechanism of chronic cadmium-induced hepatotoxicity and renal toxicity and protection by antioxidants. Toxicol Appl Pharmacol 1999;154:256-63.

Jarup L. Cadmium overload and toxicity. Nephrol Dial Transplant 2002;17:35-9.

Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 1995;18:321-36.

Larson RA. The antioxidants of higher pants. Phytochemistry 1988;27:969-78.

Bindu SM, Khadkikar P. Role of antioxidants and nutrition in oxidative stress. Int J Appl Pharm 2015;7:1-4.

McCord JM. Oxygen derived radicals a link between reperfusion injury an inflammation. Fed Proc 1987;46:2402-6.

Chance B, Green Stein DS, Roughton RJ. The mechanism of catalase action 1-steady state analysis. Arch Biochem 1952;37:301-39.

Casalino E, Calzaretti G, Sblano C, Landriscina C. Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicology 2002;179:37-50.

Amin A, Hamza AA, Daoud S, Hamsa W. Spirulina protects against cadmium induced hepatotoxicity in rats. Am J Pharmacol Toxicol 2006;1:21-5.