PREVENTIVE EFFECT OF CYCAS REVOLUTA IN 1,2-DIMETHYLHYDRAZINE-INDUCED COLON CANCER IN WISTAR RAT MODEL

Samit Bera; Bhaskar Das; Arnab De; Amalesh Samanta

doi:10.22159/ajpcr.2018.v11i5.24126

Authors

Samit Bera Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
Bhaskar Das Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
Arnab De Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
Amalesh Samanta Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i5.24126

Keywords:

Cycas revoluta, Methanolic extracts of C revoluta, 1, 2-Dimethylhydrazine, Colon cancer, 5-Fluorouracil

Abstract

Â Objective: The aim of this study was to evaluate the colon cancer protective activity of Cycas revoluta (Cycadaceae).

Methods: Methanolic extracts of C. revoluta (MECR) were assessed for total polyphenols and total flavonoids content. For the in vivo study, animals were divided into five groups (n=6). Group I serves as control which received 0.25% carboxymethyl cellulose solution. Groups II-V were treated with 1,2-dimethylhydrazine (DMH) which was given at the dose of 20 mg/kg b.w., s.c. once a week for 4 consecutive weeks. Aqueous suspension of MECR at a dose of 200 mg/kg/day and 400 mg/kg was administered orally to the animals in Groups III-IV every day for 16 weeks. Group V received 5-fluorouracil (5-FU) as a standard drug at a dose of 10 mg/kg b.w., per day s.c. for 16 weeks. After that, animals are sacrificed and colons are taken separately to evaluate biochemical parameters and morphological and histopathological changes.

Results: MECR contains total polyphenols (6.3Â±0.09 mg of gallic acid equivalent /g) and total flavonoids (4.6Â±0.06 mg of rutin equivalent/g). The in vivo study revealed that superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) activity were decreased in DMH Group. All these parameters were restored significantly (p<0.05) toward the near normal value on supplementation with MECR (200 and 400 mg/kg b.w.) to DMH-treated rats (Groups III and IV). In Group V, the synthetic standard drug 5-FU (10 mg/kg b.w.) also increases the activities of SOD, CAT, and GSH significantly (p<0.05) more in DMH-treated rats.

Conclusions: It can be concluded that MECR protects rat from DMH-induced colon cancer.

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