NOVEL HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR SIMPLE, ECONOMICAL, AND RAPID DETERMINATION OF FENOFIBRATE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Rani S Potawale; Tabassume I Hangad

doi:10.22159/ajpcr.2018.v11i5.24240

Authors

Rani S Potawale Department of Pharmaceutical Chemistry, Allana College of Pharmacy, Pune, Maharashtra, India.
Tabassume I Hangad Department of Pharmaceutical Chemistry, Allana College of Pharmacy, Pune, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i5.24240

Keywords:

Fenofibrate, High-performance thin-layer chromatographic, Single solvent analysis, Validation

Abstract

Â Objective: A simple, novel, sensitive, and rapid high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for quantitative determination of fenofibrate in bulk and formulations.

Methods: The chromatographic development was carried out on HPTLC plates precoated with silica gel 60 F254 using a single solvent dichloromethane as a simple mobile phase. Densitometric detection was carried out at 292 nm.

Results: Rf value of drug was found to be 0.33Â±0.02. The method was validated as per International Conference on Harmonization Guideline with respect to linearity, accuracy, precision, and robustness. The calibration curve was found to be linear over a range of 20â€“400Â° ng bandâˆ’1Â° with a regression coefficient of 0.999. The method has proved high sensitivity and specificity.

Conclusion: Proposed densitometric method was found to be new, simple, and economic for routine quantification of fenofibrate in bulk and pharmaceutical formulation.

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