EVALUATION OF IN VITRO ANTIDIABETIC AND ANTI-INFLAMMATORY ACTIVITIES OF LEAVES EXTRACT OF BOEHMERIA RUGULOSA
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i9.25906Keywords:
Anti-inflammatory, Antidiabetic, Boehmeria rugulosa, Diclofenac sodium, AcarboseAbstract
Objective: The objective of the study is to evaluate in vitro antidiabetic and anti-inflammatory activity of different extracts of leaves of Boehmeria rugulosa by different methods.
Methods: In vitro α-glucose and α-amylase were used for antidiabetic activity and lipoxygenase, and protein denaturation method of inhibition assays was used to measure anti-inflammatory activity. Successive extraction of leaves petroleum ether (PE), chloroform (CH), ethyl acetate (EA), acetone (AC), and ethanol (ETH) was performed, and extracts obtained from the extraction were applicable to these activities.
Results: The AC extract of leaves shows significantly in vitro antidiabetic activity, and AC has offered significant result 470.07±0.65 μg/mL in the inhibition of α-glucosidase and also for α-amylase assay 698.15±1.71 μg/mL. Acarbose was used as standard. In lipoxidase method, AC had shown better results and in protein denaturation method EA shown the higher inhibition (78.06±0.5 μg/ml) than the other extracts. The standard drug diclofenac sodium also offered significant inhibition against lipoxidase enzyme method with IC50 value 21.76±1.29 μg/mL.
Conclusion: These findings suggest that the AC and EA possess potent antidiabetic and anti-inflammatory activities in vitro conditions.
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Semwal DK, Rawat U, Semwal R, Singh R, Krishan P, Singh GJ. Chemical constituents from the leaves Of Boehmeria rugulosa with antidiabetic and antimicrobial activities. J Asian Nat Prod Res 2009;11:1045-55.
Das P, Devi PV, Yasmine Y. Assessment of in vitro anti-diabetic activity of Ficus glomerata Merina. Pharm Lett 2016;8:267-72.
Day C, Bailey CJ. Hypoglycemic agents from traditional plant treatments for diabetes. Int Ind Biotech 1998;50:5-8.
Gray AM, Flatt PR. Nature’s own pharmacy: The diabetes perspective. Proc Nutr Soc 1997;56:507-17.
Swanston-Flatt SK, Flatt PR, Day C, Bailey CJ. Traditional dietary adjuncts for the treatment of diabetes mellitus. Proc Nutr Soc 1991;50:641-50.
Leelaprakash G, Dass SM. In-vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2010;3:189-96.
Ingle PV, Patel DM. C-reactive protein in various disease condition - an overview. Asian J Pharm Clin Res 2011;4:9-13.
Anoop MV, Bindu AR. In-vitro anti-inflammatory activity studies on Syzygium zeylanicum (L.) DC Leaves. Int J Pharm Res Rev 2015;4:18-27.
Anchal C, Shukla A, Shukla RK, Tyagi R. Evaluation of Antioxidant and antidiabetic capacity of plant Boehmeria rugulosa bark. Int J Chemtech Res 2017;10:324-32.
Hansawasdi C, Kawabata J, Kasai T. Alpha amylase inhibitiors from Roselle (Hibiscus sabdariffa). Biosci Biotechnol Biochem 2000;64:1041-3.
Rengasamy S, Thangaprakasam U. Isolation, screening and determination of a-amylase activity from Marine streptomyces species. Int J Pharm Pharm Sci 2018;10:122-7.
Andrade-Cetto A, Becerra-Jimenez J, Cardenas-Vazquez R. Alpha-glucosidase inhibiting activity of some Mexican plants used in the treatment of Typa 2 diabetes. J Ethnopharmacol 2008;116:27-32.
Li D, Ni JM. Preliminary study of an alpha-glucosidase inhibition from the roots and stems of polygonatum sibiricum Red. Asian J Tradit Med 2008;3:179-85.
Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.
Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146-55.
Cashman JN. The mechanisms of action of NSAIDs in analgesia. Drugs 1996;52:13-23.
Singh N, Gupta AK, Juyal V, Deepak P, Gahlot M. Phytochemical screening and blood glucose level lowering effect of Bergenia ligulata root extracts. J Nat Remedies 2011;11:19-23.
Reddenna P, Whelan J, Maddipati KR, Reddy CC. Purification of arachidonate 5-LOX from potato tubers. Methods Enzymol 1990;187:268-77.
Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Dikshit VJ, Mungantiwar AA, et al. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara Roxb loud wood oil. Indian J Exp Biol 1999;37:258-61.
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