THE INHIBITION OF POLYISOPRENOIDS FROM NYPA FRUTICANS LEAVES ON CYCLOOXYGENASE 2 EXPRESSION OF WIDR COLON CANCER CELLS

Dini Permata Sari; Mohammad Basyuni; Poppy Anjelisa Zaitun Hasibuan; Ridha Wati

doi:10.22159/ajpcr.2018.v11i8.26098

Authors

Dini Permata Sari Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia. http://orcid.org/0000-0003-1120-8571
Mohammad Basyuni Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.
Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia.
Ridha Wati Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i8.26098

Keywords:

Cytotoxic, Polyisoprenoids, Nypa fruticans, Cyclooxygenase-2

Abstract

Objective: The objective of the study was to investigate the inhibitory activity of polyisoprenoids from Nypa fruticans leaves on the expression of cyclooxygenase 2 (COX-2) against colon cancer cells.

Methods: Anticancer activity performed was tested by dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method on colon cancer cell WiDr. The expression of COX-2 was observed by the immunocytochemistry method.

Result: Polyisoprenoids from N. fruticans leaves exhibit anticancer activity on WiDr cells through inhibition of COX-2 expression with IC50 180.186Â±7.16 Î¼g/ml.

Conclusions: This study showed that polyisoprenoids from N. fruticans leaves promise chemopreventive agents for colon cancer through COX-2 inhibition.

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